Abstract：
:We compared commercial kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing. Purification of DNA was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. The choice of extraction kit had little effect on sequencing quality and coverage across drastically different replicons, except for an apparent depletion of small plasmids (<5 kb) during precipitation-based extractions. Sequencing coverage provided copy-number estimates for small plasmids that were consistently higher than those from quantitative real-time PCR.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Becker L,Steglich M,Fuchs S,Werner G,Nübel Udoi
10.1038/srep28063subject
Has Abstractpub_date
2016-06-17 00:00:00pages
28063issn
2045-2322pii
srep28063journal_volume
6pub_type
杂志文章abstract：:The functions of female song found in a few cicadas have rarely been studied. In the cicada Subpsaltria yangi we investigated the acoustic behaviour and signal structure of songs produced by females, the phonotaxis of males, and mate choice, as well as the selective pressure imposed on this species by predators. Pair-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06825-5
更新日期：2017-07-25 00:00:00
abstract：:The considerable range of genetic variation in human populations may partly reflect distinctive processes of adaptation to variable environmental conditions. However, the adaptive genomic signatures remain to be completely elucidated. This research explores candidate loci under selection at the population level by cha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22468
更新日期：2016-03-02 00:00:00
abstract：:5-hydroxymethylation (5-hmC) is an epigenetic modification on DNA that results from the conversion of 5-methylcytosine by Ten-Eleven Translocation (TET) proteins. 5-hmC is widely present in the brain and is subjected to dynamic regulation during development and upon neuronal activity. It was recently shown to be invol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19806-z
更新日期：2018-01-26 00:00:00
abstract：:There are currently no licensed therapeutic treatment or preventive vaccines against Ebolavirus disease, and the 2013-2016 West African outbreak of Ebolavirus disease spread rapidly and resulted in almost 30,000 cases and more than 11,000 deaths. However, the devastating outbreak has spurred the development of novel E...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31003-6
更新日期：2018-08-20 00:00:00
abstract：:Range-wide population studies of wide spread species are often associated with complex diversity patterns resulting from genetically divergent evolutionary significant units (ESUs). The compound evolutionary history creating such a pattern of diversity can be inferred through molecular analyses. Asparagus cochinchinen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46275-9
更新日期：2019-07-05 00:00:00
abstract：:Understanding the genetic basis of phenotypic diversity is a challenge in contemporary biology. Domestication provides a model for unravelling aspects of the genetic basis of stress sensitivity. The ancestral Red Junglefowl (RJF) exhibits greater fear-related behaviour and a more pronounced HPA-axis reactivity than it...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15345
更新日期：2015-10-16 00:00:00
abstract：:The General Unified Threshold model for Survival (GUTS) integrates previously published toxicokinetic-toxicodynamic models and estimates survival with explicitly defined assumptions. Importantly, GUTS accounts for time-variable exposure to the stressor. We performed three studies to test the ability of GUTS to predict...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29178
更新日期：2016-07-06 00:00:00
abstract：:The paper presents the procedure for planning an experiment to create standard sets of reagents for a technetium-99m generator based on glucose derivatives. All stages are presented from researching the required quantities of a substance, a reducing agent, a stabilizer and auxiliary components to developing lyophilize...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61707-7
更新日期：2020-03-20 00:00:00
abstract：:Stem cell therapy has long been considered a promising mode of treatment for many incurable diseases. Human mesenchymal stem cells (hMSCs) have provided the most promising results to date for regenerative medicine. Nevertheless, due to several obstacles such as difficulty in sourcing and characterizing hMSCs, they rem...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42669-x
更新日期：2019-04-16 00:00:00
abstract：:Electron- or X-ray-induced characteristic X-ray analysis has been widely used to determine chemical compositions of materials in vast research fields. In recent years, analysis of characteristic X-rays from muonic atoms, in which a muon is captured, has attracted attention because both a muon beam and a muon-induced c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15719-5
更新日期：2017-11-13 00:00:00
abstract：:We advance the concept that the charge factors of the simple overlap model and the polarizabilities of Judd-Ofelt theory for the luminescence of europium complexes can be effectively and uniquely modeled by perturbation theory on the semiempirical electronic wave function of the complex. With only three adjustable con...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13695
更新日期：2015-09-02 00:00:00
abstract：:Filth flies cause billions of dollars of losses annually to the animal production industry. Fluralaner is a relatively new pesticide currently sold for control of fleas, ticks, and mites on companion animals and poultry. We examined the efficacy of fluralaner against three species of filth flies. Insecticide-susceptib...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68121-z
更新日期：2020-07-07 00:00:00
abstract：:Silicon nanocrystals offer huge advantages compared to other semi-conductor quantum dots as they are made from an abundant, non-toxic material and are compatible with silicon devices. Besides, among a wealth of extraordinary properties ranging from catalysis to nanomedicine, metal nanoparticles are known to increase t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02672
更新日期：2013-01-01 00:00:00
abstract：:In situ reduction of selenite to elemental selenium (Se(0)), by microorganisms in sediments and soils is an important process and greatly affects the environmental distribution and the biological effects of selenium. However, the mechanism behind such a biological process remains unrevealed yet. Here we use Shewanella...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03735
更新日期：2014-01-17 00:00:00
abstract：:In the field of neuroprosthetics, the current state-of-the-art method involves controlling the prosthesis with electromyography (EMG) or electrooculography/electroencephalography (EOG/EEG). However, these systems are both expensive and time consuming to calibrate, susceptible to interference, and require a lengthy lea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73250-6
更新日期：2020-10-01 00:00:00
abstract：:In this paper, we demonstrate a novel dual-pump approach to generate robust optical frequency comb with varying free spectral range (FSR) spacing in a CMOS-compatible high-Q micro-ring resonator (MRR). The frequency spacing of the comb can be tuned by an integer number FSR of the MRR freely in our dual-pump scheme. Th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28501
更新日期：2016-06-24 00:00:00
abstract：:Nutritional supplements may be important on cognition but the evidence is heterogeneous. This meta-analysis aimed (1) to determine whether nutritional supplements provided to pregnant women or young children could improve cognitive development of children in developing countries, and (2) to explore how supplementation...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-017-11023-4
更新日期：2017-09-06 00:00:00
abstract：:Impulsivity is a naturally occurring behavior that, when accentuated, can be found in a variety of neuropsychiatric disorders. The expression of trait impulsivity has been shown to change with a variety of factors, such as age and sex, but the existing literature does not reflect widespread consensus regarding the inf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35537-7
更新日期：2018-12-05 00:00:00
abstract：:In situ measurements by the Curiosity rover provide a unique opportunity for studying the effects of dust on assets placed at the surface of Mars. Here we use in situ measurements of solar UV radiation to quantify the seasonal and interannual variability of dust accumulation on the sensor on the rover deck. We show th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35946-8
更新日期：2018-12-04 00:00:00
abstract：:Hysteresis, which is the history dependence of physical systems, is one of the most important topics in physics. Interestingly, bi-stability of plasma with a huge hysteresis loop has been observed in inductive plasma discharges. Despite long plasma research, how this plasma hysteresis occurs remains an unresolved ques...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15254
更新日期：2015-10-20 00:00:00
abstract：:Partial suppression of the spectral diffusion of quantum dot (QD) excitons tuned to resonance of a nano-photonic cavity is reported. The suppression is caused by the Purcell enhancement of the QD-exciton recombination rate, which alters the rate of charging of the solid-state environment by the QD itself. The effect c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37677-2
更新日期：2019-02-04 00:00:00
abstract：:Efficient control of magnetism with electric means is a central issue of current spintronics research, which opens an opportunity to design integrated spintronic devices. However, recent well-studied methods are mostly based on electric-current injection, and they are inevitably accompanied by considerable energy loss...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46009-x
更新日期：2019-07-02 00:00:00
abstract：:Given new distribution patterns of the endangered North Atlantic right whale (NARW; Eubalaena glacialis) population in recent years, an improved understanding of spatio-temporal movements are imperative for the conservation of this species. While so far visual data have provided most information on NARW movements, pas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13359-3
更新日期：2017-10-18 00:00:00
abstract：:We study the dynamics of an ultrafast single photon pulse in a one-dimensional waveguide two-point coupled with a Jaynes-Cummings system. We find that for any single photon input the transmissivity depends periodically on the separation between the two coupling points. For a pulse containing many plane wave components...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33867
更新日期：2016-09-22 00:00:00
abstract：:Anisotropically conductive materials are important components in subminiature devices. However, at this stage, some defects have limited practical applications of them, especially low anisotropic degree and high cost. Here, we report novel tricolor flag-liked microribbons array prepared by electrospinning technique. T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14583
更新日期：2015-09-28 00:00:00
abstract：:A new electrically small antenna with size ka = 0.415 is presented, fabricated and measured in this work. This is intrinsically matched to 50 Ω, has omni-directional and linear-polarized radiation pattern in the horizontal plane with maximum directivity of 1.75 dBi and simulated radiation efficiency of 93%. The antenn...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33388-w
更新日期：2018-10-09 00:00:00
abstract：:Cardiopulmonary resuscitation (CPR) training and its quality are critical in improving the survival rate of cardiac arrest. This randomized controlled study investigated the efficacy of a newly developed CPR training program for the public in a Taiwanese setting. A total of 832 adults were randomized to either a tradi...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-020-67193-1
更新日期：2020-06-22 00:00:00
abstract：:Platelet transfusions can cause adverse reactions in their recipients, including transfusion-related acute lung injury (TRALI). The pathophysiology of TRALI depends on a number of signaling pathways and the inflammatory role played by blood platelets remains controversial. Platelets are important in inflammation, part...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49040-0
更新日期：2019-08-29 00:00:00
abstract：:Here we report two types of defect-induced photoluminescence (PL) blinking behaviors observed in single epitaxial InGaAs quantum dots (QDs). In the first type of PL blinking, the "off" period is caused by the trapping of hot electrons from the higher-lying excited state (absorption state) to the defect site so that it...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08898
更新日期：2015-03-10 00:00:00
abstract：:How the human auditory system learns to map complex pinna-induced spectral-shape cues onto veridical estimates of sound-source elevation in the median plane is still unclear. Earlier studies demonstrated considerable sound-localisation plasticity after applying pinna moulds, and to altered vision. Several factors may ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36422-z
更新日期：2018-12-18 00:00:00
