Abstract:
:Infection of C3H mice with Borrelia burgdorferi, the causative agent of Lyme disease, reliably produces an infectious arthritis and carditis that peak around 3 weeks postinfection and then spontaneously resolve. Macrophage polarization has been suggested to drive inflammation, the clearance of bacteria, and tissue repair and resolution in a variety of infectious disease models. During Lyme disease it is clear that macrophages are capable of clearing Borrelia spirochetes and exhausted neutrophils; however, the role of macrophage phenotype in disease development or resolution has not been studied. Using classical (NOS2) and alternative (CD206) macrophage subset-specific markers, we determined the phenotype of F4/80(+) macrophages within the joints and heart throughout the infection time course. Within the joint, CD206(+) macrophages dominated throughout the course of infection, and NOS2(+) macrophage numbers became elevated only during the peak of inflammation. We also found dual NOS2(+) CD206(+) macrophages which increased during resolution. In contrast to findings for the ankle joints, numbers of NOS2(+) and CD206(+) macrophages in the heart were similar at the peak of inflammation. 5-Lipoxygenase-deficient (5-LOX(-/-)) mice, which display a failure of Lyme arthritis resolution, recruited fewer F4/80(+) cells to the infected joints and heart, but macrophage subset populations were unchanged. These results highlight differences in the inflammatory infiltrates during Lyme arthritis and carditis and demonstrate the coexistence of multiple macrophage subsets within a single inflammatory site.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lasky CE,Olson RM,Brown CRdoi
10.1128/IAI.00369-15subject
Has Abstractpub_date
2015-07-01 00:00:00pages
2627-35issue
7eissn
0019-9567issn
1098-5522pii
IAI.00369-15journal_volume
83pub_type
杂志文章abstract::Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with acrylamide copolymerized with gelatin (substrate-SDS-PAGE) were combined to evaluate the proteinases of both long-term-grown and fresh isolates of Trichomonas vaginalis. This two-dimensional substrate-SDS-PAGE resolved a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.157-162.1990
更新日期:1990-01-01 00:00:00
abstract::Tetanus neurotoxin (TeNT) and botulinum neurotoxin (BoNT) are clostridial neurotoxins (CNTs) responsible for the paralytic diseases tetanus and botulism, respectively. CNTs are AB toxins with an N-terminal zinc-metalloprotease light chain that is linked by a disulfide bond to a C-terminal heavy chain that includes a t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01539-13
更新日期:2014-02-01 00:00:00
abstract::The gram-negative bacterium nontypeable Haemophilus influenzae (NTHI) is the predominant pathogen in chronic otitis media with effusion and, with Streptococcus pneumoniae and Moraxella catarrhalis, is a causative agent of acute otitis media. To identify potential virulence determinants, bacterial gene expression was m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3454-3462.2003
更新日期:2003-06-01 00:00:00
abstract::Bordetella parapertussis, a respiratory tract pathogen commonly regarded as noninvasive, was found to invade HeLa 229 cell monolayers. Following treatment of the monolayers with gentamicin, numbers of viable B. parapertussis recovered were comparable to those of invasive Salmonella and Shigella isolates. Invasion occu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1240-1247.1989
更新日期:1989-04-01 00:00:00
abstract::The effect of route of administration, dosage, and number of boosts employed during immunization with the polymyxin-release form of Escherichia coli heat-labile (LT) enterotoxin on the degree and duration of protection afforded was evaluated in rats which were challenged by the ligated loop technique. Increasing the b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.81-86.1980
更新日期:1980-01-01 00:00:00
abstract::Streptococcus pneumoniae is a major cause of illness and death in children in developing countries. In these children, zinc deficiency is associated with an increased risk of acute respiratory tract infections, which can be reduced by daily zinc administration. Severe infections decrease zinc levels in plasma and may ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2009-2013.2003
更新日期:2003-04-01 00:00:00
abstract::A major goal of current vaccine development is the induction of strong immune responses against protective antigens delivered by mucosal routes. One of the most promising approaches in that respect relies on the use of live recombinant vaccine carriers. In this study, Mycobacterium bovis BCG was engineered to produce ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5669-5676.1998
更新日期:1998-12-01 00:00:00
abstract::We have developed an improved scheme for the classification of environmental and clinical isolates of Histoplasma capsulatum that is based on analysis of mitochondrial DNA (mtDNA) and ribosomal DNA (rDNA). Strains were initially divided into mtDNA groups according to restriction digests of whole-cell DNA and Southern ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1409-1412.1989
更新日期:1989-05-01 00:00:00
abstract::The salmonid macrophage-like cell line RTS-11 and purified trout pronephros phagocytes were used to analyze in vitro entry and survival of two Streptococcus iniae serotypes. Efficient invasion by S. iniae occurred in both cells, but only the type II strain persisted in pronephros phagocytes for at least 48 h. Ex vivo ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2318-2325.2003
更新日期:2003-05-01 00:00:00
abstract::Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05851-11
更新日期:2012-02-01 00:00:00
abstract::Serotype b-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans Y4 consists of D-fucose and L-rhamnose. To clarify the role of SPA in phagocytosis of the organism by human polymorphonuclear leukocytes (PMNs), monoclonal antibodies (MAbs) against SPA and SPA-defective mutants, which were constr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4589-4594.1995
更新日期:1995-12-01 00:00:00
abstract::A significant clinical complication of pulmonary infections with Klebsiella pneumoniae is peripheral blood dissemination, resulting in a systemic infection concurrent with the localized pulmonary infection. In this context, little is known about the role of tumor necrosis factor receptor 1 (TNFR1)-mediated innate immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.4891-4900.2003
更新日期:2003-09-01 00:00:00
abstract::The immunogenicity of the two major group 19 pneumococcal polysaccharides, types 19F and 19A, was studied in human volunteers. Antibody responses after vaccination with either 50 micrograms of type 19F polysaccharide, 50 micrograms of type 19A polysaccharide, or 25 micrograms of both polysaccharides premixed in a sing...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
doi:10.1128/IAI.36.3.1261-1262.1982
更新日期:1982-06-01 00:00:00
abstract::Pseudomonas aeruginosa exotoxin A (PA), a potent protein synthesis inhibitor, was found to be a weak T-cell mitogen for murine splenocytes. Maximal stimulation of [3H]thymidine incorporation was obtained with 10 to 100 ng of toxin per ml following a 4-day induction. PA was also shown to be a polyclonal activator of cy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.213-218.1988
更新日期:1988-01-01 00:00:00
abstract::A 30-amino-acid peptide corresponding to variable domain IV (VD IV) of the major outer membrane protein of Chlamydia trachomatis serovar E was conjugated to keyhole limpet hemocyanin (KLH) and used to immunize mice. The resulting antisera (anti-KLH-VD IV sera) recognized all 15 serovars of C. trachomatis when assayed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3428-3432.1992
更新日期:1992-08-01 00:00:00
abstract::It is not clear how the host initially recognizes and responds to infection by gram-negative pathogenic Brucella spp. It was previously shown (D. S. Weiss, B. Raupach, K. Takeda, S. Akira, and A. Zychlinsky, J. Immunol. 172:4463-4469, 2004) that the early macrophage response against gram-negative bacteria is mediated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5137-5143.2005
更新日期:2005-08-01 00:00:00
abstract::Attachment of Fusobacterium nucleatum to various oral surfaces is mediated by several adhesins anchored on its outer surface. Monoclonal antibodies (MAbs) were prepared and used to identify the putative galactose-binding adhesin of F. nucleatum PK1594. Four unique MAbs, 8G7, 26B9, 28G11, and 29D4, were isolated on the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5231-5237.1997
更新日期:1997-12-01 00:00:00
abstract::The presence of choriogonadotropin- and alpha-subunit-like materials in two species of bacteria identified as Staphylococcus simulans and Streptococcus faecalis have been demonstrated by the indirect fluorescein-labeled and the indirect peroxidase-labeled immunocytochemical techniques, utilizing antiserum for human ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.487-494.1981
更新日期:1981-01-01 00:00:00
abstract::Gardnerella vaginalis, the bacterial species most frequently isolated from women with bacterial vaginosis (BV), produces a cholesterol-dependent cytolysin (CDC), vaginolysin (VLY). At sublytic concentrations, CDCs may initiate complex signaling cascades crucial to target cell survival. Using live-cell imaging, we obse...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00627-13
更新日期:2013-12-01 00:00:00
abstract::We have investigated the roles of gamma interferon (IFN-gamma) and interleukin-4 (IL-4) in host defense against Brugia malayi. Our data suggest that the lack of either IFN-gamma or IL-4 prolongs the time required to achieve sterile immunity, suggesting that both canonical type 1 and type 2 responses are involved in th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.3034-3035.2000
更新日期:2000-05-01 00:00:00
abstract::Erythrocytic malaria parasites degrade hemoglobin as a principal source of amino acids for parasite protein synthesis. We have previously shown that a Plasmodium falciparum trophozoite cysteine proteinase, now termed falcipain, is required for hemoglobin degradation, and we have hypothesized that this proteinase is re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.6.2120-2125.1995
更新日期:1995-06-01 00:00:00
abstract::Cell-mediated immunity to varicella-zoster (V-Z) virus in persons immune to varicella has been demonstrated, using a tissue culture technique. Cell-mediated immunity was reflected by the ability of peripheral leukocytes (lymphocytes and monocytes) from human donors to inactivate V-Z virus. Leukocytes were stimulated b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1549-1553.1976
更新日期:1976-06-01 00:00:00
abstract::By subcutaneous treatment with an aqueous solution of 6-O-stearoyl-N-acetylmuramyl-L-alanyl-D-isoglutamine [6-O-CH3-(CH2)16-CO-MurNAc-L-Ala-D-isoGln] [referred to here as L18-MDP(Ala)], an augmentation of the resistance of mice to Escherichia coli, Pseudomonas aeruginosa. Staphylococcus aureus, and Candida albicans in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.292-300.1982
更新日期:1982-07-01 00:00:00
abstract::K99 prepared by acid precipitation hemagglutinated guinea pig erythrocytes, whereas K99 prepared by chromatography on diethylaminoethyl-Sephadex did not. K99 purified by either procedure hemagglutinated horse erythrocytes. K99 prepared by acid precipitation contained a second antigen not presnet in the K99 prepared by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.555-559.1978
更新日期:1978-11-01 00:00:00
abstract::The typically recovered quantity of nontypeable Haemophilus influenzae (NTHi) bacteria in an ex vivo middle ear (ME) aspirate from the chinchilla model of experimental otitis media is insufficient for direct analysis of gene expression by microarray or of lipopolysaccharide glycoforms by mass spectrometry. This prompt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00318-07
更新日期:2007-08-01 00:00:00
abstract::The serotype-specific polysaccharide of Streptococcus mutans AHT (serotype a) was shown to be loosely associated with the cell surface of this organism. The antigen was extracted from whole cells by boiling in sodium acetate buffer, pH 4.0, for 10 min. The purified product was found to be a diheteroglycan of galactose...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1177-1185.1979
更新日期:1979-12-01 00:00:00
abstract::The hagfish, Eptatretus stoutii, has been shown to synthesize bactericidal antibodies after injections of gram-negative organisms. These bactericidins could be detected as early as 2 days after the primary injection. Secondary responses were accelerated, although titers were not significantly higher than those reached...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.2.160-166.1971
更新日期:1971-08-01 00:00:00
abstract::Edema disease (ED) of weanling pigs is caused by an infection with Escherichia coli that produces Shiga-like toxin II variant (SLT-IIv). Pathology identical to that caused by ED can be duplicated in pigs that are injected with less than 10 ng of purified SLT-IIv per kg of body weight. Therefore, SLT-IIv was mutated to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.485-490.1992
更新日期:1992-02-01 00:00:00
abstract::We constructed hha derivatives from both a clinical uropathogenic Escherichia coli isolate (strain FVL4) and a wild E. coli strain causing bovine diarrhea (strain CCB21) and analyzed the effect of the hha allele on the expression of the different virulence factors exhibited by these strains. Expression of hemolysin an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2881-2884.1996
更新日期:1996-07-01 00:00:00
abstract::The superoxide anion (O2-)-generating activity of guinea pig macrophages stimulated by wheat germ agglutinin (WGA), immune complexes, or phorbol myristate acetate (PMA) was studied after short- and long-term exposures of the cells to muramyl dipeptide (MDP) or lipopolysaccharide (LPS). Neither MDP nor LPS alone induce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.82-86.1984
更新日期:1984-07-01 00:00:00