Abstract:
:Thymidylate synthase (TYMS) is an important enzyme for 5-fluorouracil (5-FU) metabolism in metastatic colorectal cancer (mCRC) patients. The search for this enzyme in circulating tumor cells (CTCs) can be a powerful tool to follow-up cancer patients. mCRC patients were enrolled before the beginning of 5-FU-based chemotherapy. The blood was filtered on Isolation by Size of Epithelial Tumor Cells (ISET), and the analysis of TYMS expression in CTCs was made by immunocytochemistry. Additionally, we verified TYMS staining in primary tumors and metastases from the same patients. There were included 54 mCRC patients and 47 of them received 5-FU-based chemotherapy. The median CTCs number was 2 per mL. We were not able to analyze immunocytochemistry in 13 samples (9 patients with absence of CTCs and 4 samples due to technical reasons). Therefore, TYMS expression on CTCs was analyzed in 34 samples and was found positive in 9 (26.5%). Six of these patients had tumor progression after treatment with 5-FU. We found an association between CTC TYMS staining and disease progression (DP), although without statistical significance (P = 0.07). TYMS staining in primary tumors and metastases tissues did not have any correlation with disease progression (P = 0.67 and P = 0.42 respectively). Patients who had CTC count above the median (2 CTCs/mL) showed more TYMS expression (P = 0.02) correlating with worse prognosis. Our results searching for TYMS staining in CTCs, primary tumors and metastases suggest that the analysis of TYMS can be useful tool as a 5-FU resistance predictor biomarker if analyzed in CTCs from mCRC patients.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Abdallah EA,Fanelli MF,Buim ME,Machado Netto MC,Gasparini Junior JL,Souza E Silva V,Dettino AL,Mingues NB,Romero JV,Ocea LM,Rocha BM,Alves VS,Araújo DV,Chinen LTdoi
10.1002/ijc.29495subject
Has Abstractpub_date
2015-09-15 00:00:00pages
1397-405issue
6eissn
0020-7136issn
1097-0215journal_volume
137pub_type
杂志文章abstract::Recombinant human interferon beta (rIFN-beta) inhibited in a time- and dose-dependent manner the proliferation of 18/18 human colon carcinoma cell lines in monolayer culture and 8/9 lines in a soft agar assay but had no effect on 4 human fibroblast cell lines. Maximal inhibition of cell proliferation by rIFN-beta requ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430331
更新日期:1989-03-15 00:00:00
abstract::By radioimmunoassay (RIA) mammary tumour virus (MTV) antigens were detected in individual milk samples of C3Hf mice, (female BALB/c X male C3Hf)F1 mice and (female C3Hf X male BALB/c)F1 mice; milk samples of BALB/c mice were negative. In the segregating backcross I population, female BALB/c X male (female BALB/c X mal...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910160606
更新日期:1975-12-15 00:00:00
abstract::We evaluated the impact of a family history of breast/ovarian cancer on the risk of secondary leukemia following breast cancer. At the Geneva cancer registry, we identified 4,397 patients diagnosed with invasive breast cancer between 1990 and 2004. Patients were followed up for leukemia until the end of 2005. Family h...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23212
更新日期:2008-03-01 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors, such as valproic acid (VPA), constitute a novel class of anticancer agents that cause an increase in acetylated histones and thus restore the expression of dormant tumor-suppressor and other genes related to cell differentiation, cell-cycle arrest or apoptosis of tumor cells. The...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25367
更新日期:2011-02-01 00:00:00
abstract::Irinotecan-based chemotherapy with bevacizumab is one of the first-line standard therapies for metastatic colorectal cancer (mCRC). TEGAFIRI (UFT/LV + irinotecan) is an irinotecan-based chemotherapy regimen. Currently, few clinical data regarding TEGAFIRI are available. This study evaluated the efficacy and safety of ...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.30127
更新日期:2016-08-15 00:00:00
abstract::The p53 tumor suppressor pathway is impaired in more than 90% of cervical cancers and cancer-derived cell lines as a result of infection by human papillomavirus (HPV). The HPV E6 oncoprotein forms complexes with p53 and promotes its degradation via ubiquitin-dependent mechanism. In our study, we attempted to improve t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26013
更新日期:2012-01-15 00:00:00
abstract::Hyaluronan (HA) has been shown to play crucial roles in the tumorigenicity of malignant tumors. Previous studies demonstrated that inhibition of HA suppressed the tumorigenicity of various malignant tumors including breast cancer. 4-methylumbelliferone (MU) has been reported to inhibit HA synthesis in several cell typ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26014
更新日期:2012-01-15 00:00:00
abstract::Cervical mucosal expression of cytokines involved in mediating cellular immunity is believed to influence the persistence of human papillomavirus (HPV) infection, a necessary prerequisite for the development of cervical intraepithelial neoplasia (CIN). Additionally, regulatory T (Treg) cells are increasingly understoo...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.24117
更新日期:2009-03-15 00:00:00
abstract::Small cell lung cancers (SCLCs) and extrapulmonary small cell cancers (SCCs) are very aggressive tumors arising de novo as primary small cell cancer with characteristic genetic lesions in RB1 and TP53. Based on murine models, neuroendocrine stem cells of the terminal bronchioli have been postulated as the cellular ori...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29835
更新日期:2016-02-15 00:00:00
abstract::During 2000, marine antitumor pharmacology research aimed at the discovery of novel antitumor agents was published in 85 peer-reviewed articles. The purpose of this article is to present a structured review of the antitumor and cytotoxic properties of 143 marine natural products, many of them novel compounds that belo...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.11080
更新日期:2003-06-20 00:00:00
abstract::The effect of linoleic acid (LA) on growth and transformation of IEC6 intestinal cells was examined. IEC6 cells expressed mRNAs of 15-lipooxygenase (LOX15) and peroxisome proliferator-activated receptor (PPAR)gamma but not COX-2. Cell growth was suppressed by LA in a dose-dependent manner in IEC6 cells. Three-week tre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21393
更新日期:2006-02-01 00:00:00
abstract::To examine the relationship between human T-lymphotrophic virus type I (HTLV-I) proviral DNA and its expression in the lymph nodes, HTLV-I DNA and tax/rex mRDA were directly amplified by polymerase chain reaction in situ hybridization (PCR/ISH), and reverse transcription (RT)-PCR/ISH [RT-PCR/ISH]. We studied 24 lymph ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960328)66:1<18::AID-IJC4
更新日期:1996-03-28 00:00:00
abstract::In mice as well as humans, cytotoxic T lymphocytes (CTL) specific for wild-type-sequence (wt) p53 peptides have been shown to react against a wide range of tumors, but not normal cells. As such, they are attractive candidates for developing broadly applicable cancer vaccines. Of particular interest is the potential of...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20686
更新日期:2005-03-01 00:00:00
abstract::China was one of the countries with the highest esophagus cancer risk in the world during the 1970s. This report provides data on time trends of esophagus cancer incidence and mortality during the 1970s-90s in selected geographic areas of China. Information on newly diagnosed cancer cases and cancer deaths is based on...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10706
更新日期:2002-11-20 00:00:00
abstract::Rapid growth of residual tumor after partial hepatectomy has been observed during the period of liver regeneration in children with malignant embryonal hepatoblastoma. The aim of this study was to elucidate the role of hepatocyte growth-factor-scatter factor (HGF-SF) in this phenomenon. Markedly increased serum levels...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(20000115)85:2<151::AID-IJC
更新日期:2000-01-15 00:00:00
abstract::The molecular mechanisms underlying the genesis and progression of oligodendroglial tumors are poorly understood, since only restricted information on loss of heterozygosity from isolated cases is available. The commonest alterations appear to involve deletion of 1p and 19q, while loss of heterozygosity for 9p, chromo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910640311
更新日期:1995-06-22 00:00:00
abstract::The SIL gene expression is increased in multiple cancers and correlates with the expression of mitotic spindle checkpoint genes and with increased metastatic potential. SIL regulates mitotic entry, organization of the mitotic spindle and cell survival. The E2F transcription factors regulate cell cycle progression by c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23665
更新日期:2008-10-01 00:00:00
abstract::This paper deals with a spontaneous malignant transformation in one of our XP fibroblast lines. This cell line, designated XP29MA, was derived from a 14-year-old boy who did not show skin tumors or precancerous alterations either at the time of clinical examination or when the biopsy was taken. We have compared the fo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910310603
更新日期:1983-06-15 00:00:00
abstract::Pesticide exposure has been associated with non-Hodgkin lymphoma (NHL) risk in a number of studies, and two recent studies suggest that the increased risk may be confined to those with a history of asthma. We examined the interaction between occupational pesticide exposure and atopy on risk of NHL in an Australian pop...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22602
更新日期:2007-05-15 00:00:00
abstract::In chronic lymphocytic leukemia (CLL) cells, both interleukin-6 (IL-6) and the B-cell receptor (BCR) activate Janus kinase 2 (JAK2) and induce the phosphorylation of signal transduction and activator of transcription 3 (STAT3) on tyrosine 705 residues. However, whereas IL-6 phosphorylates STAT3 within 15 min, stimulat...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30892
更新日期:2017-11-15 00:00:00
abstract::During 1984-88 a population-based case-control study was carried out in The Netherlands in collaboration with the International Agency for Research on Cancer in order to further explore the possible relationship between diet and exocrine pancreatic carcinoma. Past habitual dietary intake was assessed in 164 cases and ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910460319
更新日期:1990-09-15 00:00:00
abstract::Vitamin D is well known for its important role in calcium and phosphor homeostasis. Recent research suggests that vitamin D also prevent some type of cancers. We studied solar vitamin D effective UV radiation (VD dose), dietary vitamin D, sun-seeking holidays, use of solarium, frequency of sunburn and breast cancer ri...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.25463
更新日期:2011-03-15 00:00:00
abstract::When sensitized leukocytes were re-exposed to EBV antigen or to tuberculin-purified protein derivatives, they produced lymphokines, including the migration inhibition factor, which inhibited the migration of guinea-pig peritoneal exudate cells, and other lymphokines with the characteristics of interferon, which inhibi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200106
更新日期:1977-07-15 00:00:00
abstract::Multiple myeloma (MM) is a B lymphocyte malignancy that remains incurable despite extensive research efforts. This is due, in part, to frequent disease recurrences associated with the persistence of myeloma cancer stem cells (mCSCs). Bone marrow mesenchymal stromal cells (BMSCs) play critical roles in supporting mCSCs...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29145
更新日期:2015-03-01 00:00:00
abstract::There is strong evidence of a protection of high consumption of plant foods against squamous cell carcinoma of the oesophagus, but the respective role of fruits and vegetables is not well elucidated. In the time period 1996-2003, a case-control study designed in order to address this question was conducted in Uruguay....
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20950
更新日期:2005-08-10 00:00:00
abstract::Polyunsaturated fatty acids (PUFAs) have been suggested, on the basis of animal-model studies, to be related not only to cancer development but also to chemotherapeutic effects. Controversy persists, however, as to which types of PUFAs are beneficial in terms of chemosensitivity. In this study, we used the NIH3T3 cell...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970127)70:3<357::aid-ijc
更新日期:1997-01-27 00:00:00
abstract::The International Classification of Childhood Cancer (ICCC) updates the widely used Birch and Marsden classification scheme. ICCC is based on the second edition of the International Classification of Diseases for Oncology (ICD-O-2). The purpose of the new classification is to accommodate important changes in recogniti...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0215(19961211)68:6<759::AID-IJC
更新日期:1996-12-11 00:00:00
abstract::We examined pp60c-src protein kinase activity in human gastric carcinoma cell lines and gastric carcinoma tissues as well as normal mucosa. pp60c-src kinase activity was detected in all 5 carcinoma cell lines at various levels. Of 16 gastric carcinoma tissues, 8 showed higher pp60c-src kinase activity in tumor tissues...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910450510
更新日期:1990-05-15 00:00:00
abstract::Non-steroidal anti-inflammatory drugs (NSAIDs), including aspirin, seem to have chemopreventive properties against several types of cancer, particularly colon cancer. Persons with rheumatoid arthritis, an autoimmune disease for which NSAIDs are used commonly, have been reported to be at lower risk of colon cancer but ...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/ijc.11311
更新日期:2003-09-20 00:00:00
abstract::We studied dietary risk factors for lung cancer among never-smokers in a population-based case-control study in Stockholm, 1989-1995. Study subjects were older than 30 years of age and had never smoked regularly. A total of 124 cases (35 men, 89 women) and 235 controls (72 men, 163 women) participated. Exposure inform...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19981109)78:4<430::aid-ijc
更新日期:1998-11-09 00:00:00