Tools for metabolic engineering in Streptomyces.

abstract：:Epstein-Barr virus (EBV) is an oncogenic virus that infects more than 90% of the world's population. The proteins and miRNAs encoded by EBV are involved in multiple human malignancies. Recently R-resistance RNA-seq demonstrated that EBV-encoded circular RNAs. The current research aims to explore their functions in EBV...

journal_title：Bioengineered

authors： Qiao Y,Zhao X,Liu J,Yang W

更新日期：2019-12-01 00:00:00

abstract：:Herein, I track the evolution of synthetic biology from its earliest incarnations more than 50 years ago, through the DIYbio revolution, to the next 50 years. ...

journal_title：Bioengineered

authors： Sleator RD

更新日期：2014-03-01 00:00:00

abstract：:Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...

journal_title：Bioengineered

authors： Serpooshan V,Zhao M,Metzler SA,Wei K,Shah PB,Wang A,Mahmoudi M,Malkovskiy AV,Rajadas J,Butte MJ,Bernstein D,Ruiz-Lozano P

更新日期：2014-05-01 00:00:00

abstract：:We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*. ...

journal_title：Bioengineered

authors： Keane SM,Culligan EP,Hoffmann RF,Gahan CG,Hill C,Snelling WJ,Sleator RD

更新日期：2016-04-02 00:00:00

abstract：:Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...

journal_title：Bioengineered

authors： Welman T,Michel S,Segaren N,Shanmugarajah K

更新日期：2015-01-01 00:00:00

abstract：:Biodiesel, as one type of renewable energy, is an ideal substitute for petroleum-based diesel fuel and is usually made from triacylglycerides by transesterification with alcohols. Biodiesel production based on microbial fermentation aiming to establish more efficient, less-cost and sustainable biodiesel production str...

journal_title：Bioengineered

authors： Lin H,Wang Q,Shen Q,Zhan J,Zhao Y

更新日期：2013-09-01 00:00:00

abstract：:In this paper, a miniaturized cell suspension concentration monitoring method was investigated. The sensing unit was a carbon screen-printed electrode (CSPE) in serial with a 433MHz vacuum-packaged surface acoustic wave resonator (SAWR). SAWR provided a stable and high operating frequency, which helps to keep the stab...

journal_title：Bioengineered

authors： Li J,Feng H,Fang Y

更新日期：2015-01-01 00:00:00

abstract：:Bacterial CotA-laccases exhibit higher activity in alkaline pH and salt concentration conditions compared to laccases from white-rot fungi. They are considered as green catalysts in decolorizing of industrial dyes. However, CotA-laccases are limited due to the low yield and catalytic efficiency as the spore-bound natu...

journal_title：Bioengineered

authors： Ouyang F,Zhao M

更新日期：2019-12-01 00:00:00

abstract：:In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to...

journal_title：Bioengineered

authors： Momeni A,Neelamegham S,Parashurama N

更新日期：2017-07-04 00:00:00

abstract：:Atherosclerosis enables to alter not only the microstructural but also the physical properties of the arterial walls by plaque forming. Few studies so far have been conducted to calculate the isotropic or anisotropic mechanical properties of the healthy and atherosclerotic human coronary arteries. To date there is a p...

journal_title：Bioengineered

authors： Karimi A,Rahmati SM,Sera T,Kudo S,Navidbakhsh M

更新日期：2017-03-04 00:00:00

abstract：:Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruct...

journal_title：Bioengineered

authors： Gilmore SP,Henske JK,O'Malley MA

更新日期：2015-01-01 00:00:00

abstract：:We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...

journal_title：Bioengineered

authors： Zhou J,Liu Y,Shen J,Zhang R,Tang X,Li J,Wang Y,Huang Z

更新日期：2015-01-01 00:00:00

abstract：:The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the ...

journal_title：Bioengineered

authors： Fang H,Meng Q,Zhang H,Huang J

更新日期：2016-01-01 00:00:00

abstract：NANNOCHLOROPSIS:sp. is a green alga that is widely used in the aquaculture industry as a feed in Malaysia, but genetic engineering studies of this alga are still underexplored even though there is a growing interest in microalgae genetic engineering for various industrial purposes. This study aims to investigate the ef...

journal_title：Bioengineered

authors： Zainal Abidin AA,Suntarajh M,Balia Yusof ZN

更新日期：2020-12-01 00:00:00

abstract：:Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been ...

journal_title：Bioengineered

authors： Liu L,Yang H,Shin HD,Chen RR,Li J,Du G,Chen J

更新日期：2013-07-01 00:00:00

abstract：:Prediction of tissue damage under thermal loads plays important role for thermal ablation planning. A new methodology is presented in this paper by combing non-Fourier bio-heat transfer, constitutive elastic mechanics as well as non-rigid motion of dynamics to predict and analyze thermal distribution, thermal-induced ...

journal_title：Bioengineered

authors： Li X,Zhong Y,Smith J,Gu C

更新日期：2017-01-02 00:00:00

abstract：:The major drawback of chemical transformations for the production of 2, 5-furan dicarboxylic acid (FDCA) implies the usage of hazardous chemicals, high temperature and high pressure from nonrenewable resources. Alternate to chemical methods, biological methods are promising. Microbial FDCA production is improved throu...

journal_title：Bioengineered

authors： Rajesh RO,Godan TK,Sindhu R,Pandey A,Binod P

更新日期：2020-12-01 00:00:00

abstract：:This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the...

journal_title：Bioengineered

authors： Abd Allah EF,Alqarawi AA,Hashem A,Wirth S,Egamberdieva D

更新日期：2018-01-01 00:00:00

abstract：:D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals. However, the biochemical properties of YjhG still remain unknown. In this study, the activity assay method for YjhG was estab...

journal_title：Bioengineered

authors： Jiang Y,Liu W,Cheng T,Cao Y,Zhang R,Xian M

更新日期：2015-01-01 00:00:00

abstract：:We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also r...

journal_title：Bioengineered

authors： Ferreira JP,Noderer WL,Diaz de Arce AJ,Wang CL

更新日期：2014-05-01 00:00:00

abstract：:Botulinum toxin type A can temporarily inhibit muscle contraction. Currently, physicians administer this toxin as a bio-drug in treatment of some muscle contraction disorders. TAT-BoNT/A(1-448) is a functional recombinant protein derived from botulinum toxin light chain. Unlike the full length botulinum toxin, TAT-BoN...

journal_title：Bioengineered

authors： Saffarian P,Peerayeh SN,Amani J,Ebrahimi F,Sedighianrad H,Halabian R,Imani Fooladi AA

更新日期：2016-11-01 00:00:00

abstract：:A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...

journal_title：Bioengineered

authors： Anis SN,Iqbal NM,Kumar S,Al-Ashraf A

更新日期：2013-03-01 00:00:00

abstract：:Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased towa...

journal_title：Bioengineered

authors： Bjerga GE,Lale R,Williamson AK

更新日期：2016-01-01 00:00:00

abstract：:Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...

journal_title：Bioengineered

authors： Cheng J,Lou Y,Jiang K

更新日期：2021-12-01 00:00:00

abstract：:The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...

journal_title：Bioengineered

authors： Zhou Y,Wang J,Gao X,Wang K,Wang W,Wang Q,Yan P

更新日期：2019-12-01 00:00:00

abstract：:The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...

journal_title：Bioengineered

authors： Wang XM,Liu XM,Wang Y,Chen ZY

更新日期：2021-12-01 00:00:00

abstract：:The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in nor...

journal_title：Bioengineered

authors： Zhang J,Hou H,Chen G,Wang S,Zhang J

更新日期：2016-09-02 00:00:00

abstract：:The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fu...

journal_title：Bioengineered

authors： Rousta N,Ferreira JA,Taherzadeh MJ

更新日期：2021-12-01 00:00:00

abstract：:This paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed bypen robot tips under standard conditions. The gene expression ...

journal_title：Bioengineered

authors： Nicolini C,Singh M,Spera R,Felli L

更新日期：2013-09-01 00:00:00

abstract：:This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that ser...

journal_title：Bioengineered

authors： Guo Y

更新日期：2019-12-01 00:00:00