Tools for metabolic engineering in Streptomyces.

Abstract:

:During the last few decades, Streptomycetes have shown to be a very important and adaptable group of bacteria for the production of various beneficial secondary metabolites. These secondary metabolites have been of great interest in academia and the pharmaceutical industries. To date, a vast variety of techniques and tools for metabolic engineering of relevant structural biosynthetic gene clusters have been developed. The main aim of this review is to summarize and discuss the published literature on tools for metabolic engineering of Streptomyces over the last decade. These strategies involve precursor engineering, structural and regulatory gene engineering, and the up or downregulation of genes, as well as genome shuffling and the use of genome scale metabolic models, which can reconstruct bacterial metabolic pathways to predict phenotypic changes and hence rationalize engineering strategies. These tools are continuously being developed to simplify the engineering strategies for this vital group of bacteria.

journal_name

Bioengineered

journal_title

Bioengineered

authors

Bekker V,Dodd A,Brady D,Rumbold K

doi

10.4161/bioe.29935

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

293-9

issue

5

eissn

2165-5979

issn

2165-5987

journal_volume

5

pub_type

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