Production of L-carnitine-enriched edible filamentous fungal biomass through submerged cultivation.

abstract：:The major drawback of chemical transformations for the production of 2, 5-furan dicarboxylic acid (FDCA) implies the usage of hazardous chemicals, high temperature and high pressure from nonrenewable resources. Alternate to chemical methods, biological methods are promising. Microbial FDCA production is improved throu...

journal_title：Bioengineered

authors： Rajesh RO,Godan TK,Sindhu R,Pandey A,Binod P

更新日期：2020-12-01 00:00:00

abstract：:A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...

journal_title：Bioengineered

authors： Anis SN,Iqbal NM,Kumar S,Al-Ashraf A

更新日期：2013-03-01 00:00:00

abstract：:Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (Ans...

journal_title：Bioengineered

authors： Huerta-Saquero A,Evangelista-Martínez Z,Moreno-Enriquez A,Perez-Rueda E

更新日期：2013-01-01 00:00:00

abstract：:The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...

journal_title：Bioengineered

authors： Wang XM,Liu XM,Wang Y,Chen ZY

更新日期：2021-12-01 00:00:00

abstract：:Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

journal_title：Bioengineered

authors： Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

更新日期：2017-03-04 00:00:00

abstract：NANNOCHLOROPSIS:sp. is a green alga that is widely used in the aquaculture industry as a feed in Malaysia, but genetic engineering studies of this alga are still underexplored even though there is a growing interest in microalgae genetic engineering for various industrial purposes. This study aims to investigate the ef...

journal_title：Bioengineered

authors： Zainal Abidin AA,Suntarajh M,Balia Yusof ZN

更新日期：2020-12-01 00:00:00

abstract：:The influenza A virus is a critical public health problem that causes epidemics and pandemics, and occurs widely all over the world. Various vaccines against the virus have not provided a solution to the problem. Different approaches, particularly M2e peptide-based vaccines, are available for developing universal vacc...

journal_title：Bioengineered

authors： Kilinc YB,Akdeste ZM,Koc RC,Bagirova M,Allahverdiyev A

更新日期：2014-01-01 00:00:00

abstract：:The bioengineering tools have significant advantages through less time-consuming and utilized as a promising stage for the production of pharmaceutical bioproducts under the single platform. This review highlighted the advantages and current improvement in the plant, animal and microbial bioengineering tools and outli...

journal_title：Bioengineered

authors： Sarsaiya S,Shi J,Chen J

更新日期：2019-12-01 00:00:00

abstract：:Circular RNA (circRNA) circ-LRP6 was recently proven to be a pivotal player in various human diseases. Nevertheless, its role in esophageal squamous cell cancer (ESCC) remains unknown. In the current study, we investigated the expression level, biological function and mechanism of circ-LRP6 in ESCC. Circ-LRP6 was sign...

journal_title：Bioengineered

authors： Wang J,Zhu W,Tao G,Wang W

更新日期：2020-12-01 00:00:00

abstract：:Pancreatic adenocarcinoma (PAAD) ranks among the most lethal cancers worldwide with high mortality. A marked increase in the level of long non-coding RNA LINC00460 was reported in PAAD patients, in comparison with the healthy controls. However, the underlying mechanisms of the above phenomenon are not yet well underst...

journal_title：Bioengineered

authors： Cheng J,Lou Y,Jiang K

更新日期：2021-12-01 00:00:00

abstract：:Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...

journal_title：Bioengineered

authors： Yu P,Zhang Y,Gu D

更新日期：2017-09-03 00:00:00

abstract：:As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...

journal_title：Bioengineered

authors： Zheng Y,Yang Y,Wu S,Zhu Y,Tang X,Liu X

更新日期：2017-07-04 00:00:00

abstract：:Botulinum toxin type A can temporarily inhibit muscle contraction. Currently, physicians administer this toxin as a bio-drug in treatment of some muscle contraction disorders. TAT-BoNT/A(1-448) is a functional recombinant protein derived from botulinum toxin light chain. Unlike the full length botulinum toxin, TAT-BoN...

journal_title：Bioengineered

authors： Saffarian P,Peerayeh SN,Amani J,Ebrahimi F,Sedighianrad H,Halabian R,Imani Fooladi AA

更新日期：2016-11-01 00:00:00

abstract：:For somatic gene therapy of hemophilia B, hepatocytes as the main cellular host for expression of hFIX are attractive targets. In gene therapy protocols, an efficient expression vector equipped with cis-regulatory elements such as introns is required. With this in mind, hFIX-expressing plasmids equipped with different...

journal_title：Bioengineered

authors： Azadbakhsh AS,Sam MR,Farokhi F

更新日期：2016-11-01 00:00:00

abstract：:Engineered zinc-finger nucleases (ZFNs) are powerful tools for creating double-stranded-breaks (DSBs) in genomic DNA in a site-specific manner. These DSBs generated by ZFNs can be repaired by homology-directed repair or nonhomologous end joining, in which the latter can be exploited to generate insertion or deletion m...

journal_title：Bioengineered

authors： Haryadi R,Zhang P,Chan KF,Song Z

更新日期：2013-03-01 00:00:00

abstract：:By-products from the food sector with a high load of organic matter present both a waste-handling problem related to expenses and to the environment, yet also an opportunity. This study aims to increase the value of stale bread and brewers spent grain (BSG) by re-introducing these residues to the food production chain...

journal_title：Bioengineered

authors： Gmoser R,Fristedt R,Larsson K,Undeland I,Taherzadeh MJ,Lennartsson PR

更新日期：2020-12-01 00:00:00

abstract：:In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...

journal_title：Bioengineered

authors： Wei L,Yuanyuan H,Yanping C,Jiaojiao J,Guohua H

更新日期：2015-01-01 00:00:00

abstract：:Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased towa...

journal_title：Bioengineered

authors： Bjerga GE,Lale R,Williamson AK

更新日期：2016-01-01 00:00:00

abstract：:Lon protease is conserved from bacteria to humans and regulates cellular processes by degrading different classes of proteins including antitoxins, transcriptional activators, unfolded proteins, and free ribosomal proteins. Since we found that Lon has several putative cyclic diguanylate (c-di-GMP) binding sites and si...

journal_title：Bioengineered

authors： Osbourne DO,Soo VW,Konieczny I,Wood TK

更新日期：2014-07-01 00:00:00

abstract：:Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...

journal_title：Bioengineered

authors： Schwarz CK,Lenders MH,Smits SH,Schmitt L

更新日期：2012-09-01 00:00:00

abstract：:Efficient assembly of multiple DNA fragments is a pivotal technology for synthetic biology. A scarless and sequence-independent DNA assembly method (DATEL) using thermal exonucleases has been developed recently. Here, we present a simplified DATEL (sDATEL) for efficient assembly of unphosphorylated DNA fragments with ...

journal_title：Bioengineered

authors： Ding W,Weng H,Jin P,Du G,Chen J,Kang Z

更新日期：2017-05-04 00:00:00

abstract：:Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune syste...

journal_title：Bioengineered

authors： Jahanshahi P,Wei Q,Jie Z,Ghomeishi M,Sekaran SD,Mahamd Adikan FR

更新日期：2017-05-04 00:00:00

abstract：:In this study, a total of six fungal samples were isolated from apple, strawberry and orange pulp. DNA sequence analysis was used as molecular identification method. ITS region was aligned in DNA sequence analysis, and an algorithm sequence similarity was done using BLAST (Basic Local Alignment Search Tool) program to...

journal_title：Bioengineered

authors： Asci F,Aydin B,Akkus GU,Unal A,Erdogmus SF,Korcan SE,Jahan I

更新日期：2020-12-01 00:00:00

abstract：:Touch is produced by sensations that include approaching, sliding, pressing, and temperature. This concept has become a target of research in biotechnology, especially in the field of bionic biology. This study measured sliding and pressing with traditional tactile sensors in order to improve a machine operator's judg...

journal_title：Bioengineered

authors： Chen L,Di C,Chen X,Li Z,Luo J

更新日期：2017-01-02 00:00:00

abstract：:Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from The Ca...

journal_title：Bioengineered

authors： Cheng YB,Li S,Zhang YP,Zhu ZH,Zhao N

更新日期：2021-01-31 00:00:00

abstract：:Herein, I track the evolution of synthetic biology from its earliest incarnations more than 50 years ago, through the DIYbio revolution, to the next 50 years. ...

journal_title：Bioengineered

authors： Sleator RD

更新日期：2014-03-01 00:00:00

abstract：:The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are 3 examples of natural modular proteins with each ...

journal_title：Bioengineered

authors： Maervoet VET,Briers Y

更新日期：2017-05-04 00:00:00

abstract：:Increasing evidence has shown that the tumor microenvironment (TME) plays an important role in tumor occurrence and development and can also affect patient prognosis. In this study, we screened key prognostic genes in the breast cancer (BC) TME by analyzing the immune and stromal scores of tumor samples to detect diff...

journal_title：Bioengineered

authors： Ye Q,Han X,Wu Z

更新日期：2020-12-01 00:00:00

abstract：:The anti-inflammatory cytokine interleukin-10 and its viral homologs were chosen as model proteins for the development of drug delivery systems based on probiotic carriers like E. coli Nissle 1917, E. coli G3/10, and Saccharomyces boulardii. Exterior cytokine secretion was achieved by a modified E. coli hemolysin tran...

journal_title：Bioengineered

authors： Pöhlmann C,Thomas M,Förster S,Brandt M,Hartmann M,Bleich A,Gunzer F

更新日期：2013-05-01 00:00:00

abstract：:CRISPR (Clustered Regularly-Interspaced Short Palindromic Repeats)-Cas9 (CRISPR associated protein 9) has rapidly become the most promising genome editing tool with great potential to revolutionize medicine. Through guidance of a 20 nucleotide RNA (gRNA), CRISPR-Cas9 finds and cuts target protospacer DNA precisely 3 b...

journal_title：Bioengineered

authors： Zhang JH,Adikaram P,Pandey M,Genis A,Simonds WF

更新日期：2016-04-01 00:00:00