Recent advances in synthetic biology of cyanobacteria for improved chemicals production.

Abstract:

:Cyanobacteria are Gram-negative photoautotrophic prokaryotes and have shown great importance to the Earth's ecology. Based on their capability in oxygenic photosynthesis and genetic merits, they can be engineered as microbial chassis for direct conversion of carbon dioxide to value-added biofuels and chemicals. In the last decades, attempts have given to the application of synthetic biology tools and approaches in the development of cyanobacterial cell factories. Despite the successful proof-of-principle studies, large-scale application is still a technical challenge due to low yields of bioproducts. Therefore, recent efforts are underway to characterize and develop genetic regulatory parts and strategies for the synthetic biology applications in cyanobacteria. In this review, we present the recent advancements and application in cyanobacterial synthetic biology toolboxes. We also discuss the limitations and future perspectives for using such novel tools in cyanobacterial biotechnology.

journal_name

Bioengineered

journal_title

Bioengineered

authors

Wang F,Gao Y,Yang G

doi

10.1080/21655979.2020.1837458

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

1208-1220

issue

1

eissn

2165-5979

issn

2165-5987

journal_volume

11

pub_type

杂志文章
  • Combining MPDL3280A with adoptive cell immunotherapy exerts better antitumor effects against cervical cancer.

    abstract::As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptiv...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1230573

    authors: Zheng Y,Yang Y,Wu S,Zhu Y,Tang X,Liu X

    更新日期:2017-07-04 00:00:00

  • Rhizoremediation of Cu(II) ions from contaminated soil using plant growth promoting bacteria: an outlook on pyrolysis conditions on plant residues for methylene orange dye biosorption.

    abstract::Rhizoremediation is one of the most accepted, cost-effective bioremediation techniques focusing on the application of rhizospheric microorganisms in combination with plants for the remediation of organic and inorganic pollutants from the contaminated sites. This work focuses on isolation and identification of metal re...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1728034

    authors: Yaashikaa PR,Senthil Kumar P,Varjani S,Saravanan A

    更新日期:2020-12-01 00:00:00

  • Bioengineered collagens: emerging directions for biomedical materials.

    abstract::Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.4161/bioe.28791

    authors: Ramshaw JA,Werkmeister JA,Dumsday GJ

    更新日期:2014-07-01 00:00:00

  • Novel strategies to improve co-fermentation of pentoses with D-glucose by recombinant yeast strains in lignocellulosic hydrolysates.

    abstract::Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker's yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to th...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.21444

    authors: Oreb M,Dietz H,Farwick A,Boles E

    更新日期:2012-11-01 00:00:00

  • Use of bio-mimetic three-dimensional technology in therapeutics for heart disease.

    abstract::Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.27751

    authors: Serpooshan V,Zhao M,Metzler SA,Wei K,Shah PB,Wang A,Mahmoudi M,Malkovskiy AV,Rajadas J,Butte MJ,Bernstein D,Ruiz-Lozano P

    更新日期:2014-05-01 00:00:00

  • Effect of testosterone on the proliferation and collagen synthesis of cardiac fibroblasts induced by angiotensin II in neonatal rat.

    abstract::The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone gro...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1227141

    authors: Yang X,Wang Y,Yan S,Sun L,Yang G,Li Y,Yu C

    更新日期:2017-01-02 00:00:00

  • Genetic engineering of microorganisms for biodiesel production.

    abstract::Biodiesel, as one type of renewable energy, is an ideal substitute for petroleum-based diesel fuel and is usually made from triacylglycerides by transesterification with alcohols. Biodiesel production based on microbial fermentation aiming to establish more efficient, less-cost and sustainable biodiesel production str...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.4161/bioe.23114

    authors: Lin H,Wang Q,Shen Q,Zhan J,Zhao Y

    更新日期:2013-09-01 00:00:00

  • Chaperones in maturation of molybdoenzymes: Why specific is better than general?

    abstract::Molybdoenzymes play essential functions in living organisms and, as a result, in various geochemical cycles. It is thus crucial to understand how these complex proteins become highly efficient enzymes able to perform a wide range of catalytic activities. It has been established that specific chaperones are involved du...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1218579

    authors: Lemaire ON,Bouillet S,Méjean V,Iobbi-Nivol C,Genest O

    更新日期:2017-03-04 00:00:00

  • Non-Fourier based thermal-mechanical tissue damage prediction for thermal ablation.

    abstract::Prediction of tissue damage under thermal loads plays important role for thermal ablation planning. A new methodology is presented in this paper by combing non-Fourier bio-heat transfer, constitutive elastic mechanics as well as non-rigid motion of dynamics to predict and analyze thermal distribution, thermal-induced ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2016.1227609

    authors: Li X,Zhong Y,Smith J,Gu C

    更新日期:2017-01-02 00:00:00

  • Penaeus orientolis prawn freshness rapid determination method based on electronic nose and non-linear stochastic resonance technique.

    abstract::In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/21655979.2014.993294

    authors: Wei L,Yuanyuan H,Yanping C,Jiaojiao J,Guohua H

    更新日期:2015-01-01 00:00:00

  • CHO-gmt5, a novel CHO glycosylation mutant for producing afucosylated and asialylated recombinant antibodies.

    abstract::Engineered zinc-finger nucleases (ZFNs) are powerful tools for creating double-stranded-breaks (DSBs) in genomic DNA in a site-specific manner. These DSBs generated by ZFNs can be repaired by homology-directed repair or nonhomologous end joining, in which the latter can be exploited to generate insertion or deletion m...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.22262

    authors: Haryadi R,Zhang P,Chan KF,Song Z

    更新日期:2013-03-01 00:00:00

  • Isolation of a novel deep-sea Bacillus circulus strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production.

    abstract::The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1586055

    authors: Zhou Y,Wang J,Gao X,Wang K,Wang W,Wang Q,Yan P

    更新日期:2019-12-01 00:00:00

  • Increased recovery and improved purity of PHA from recombinant Cupriavidus necator.

    abstract::A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.22350

    authors: Anis SN,Iqbal NM,Kumar S,Al-Ashraf A

    更新日期:2013-03-01 00:00:00

  • Regulatory roles of 24-epibrassinolide in tolerance of Acacia gerrardii Benth to salt stress.

    abstract::This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2017.1297348

    authors: Abd Allah EF,Alqarawi AA,Hashem A,Wirth S,Egamberdieva D

    更新日期:2018-01-01 00:00:00

  • Scarless assembly of unphosphorylated DNA fragments with a simplified DATEL method.

    abstract::Efficient assembly of multiple DNA fragments is a pivotal technology for synthetic biology. A scarless and sequence-independent DNA assembly method (DATEL) using thermal exonucleases has been developed recently. Here, we present a simplified DATEL (sDATEL) for efficient assembly of unphosphorylated DNA fragments with ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2017.1308986

    authors: Ding W,Weng H,Jin P,Du G,Chen J,Kang Z

    更新日期:2017-05-04 00:00:00

  • Knockdown of STIM1 expression inhibits non-small-cell lung cancer cell proliferation in vitro and in nude mouse xenografts.

    abstract::Stromal interaction molecule 1 (STIM1) is a calcium-sensing protein localized in the membrane of the endoplasmic reticulum. The expression of STIM1 has been shown to be closely associated with cell proliferation. The aim of the present study was to investigate the role of STIM1 in the regulation of cancer progression ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1669518

    authors: Ge C,Zeng B,Li R,Li Z,Fu Q,Wang W,Wang Z,Dong S,Lai Z,Wang Y,Xue Y,Guo J,Di T,Song X

    更新日期:2019-12-01 00:00:00

  • Circular RNA circ-LRP6 facilitates Myc-driven tumorigenesis in esophageal squamous cell cancer.

    abstract::Circular RNA (circRNA) circ-LRP6 was recently proven to be a pivotal player in various human diseases. Nevertheless, its role in esophageal squamous cell cancer (ESCC) remains unknown. In the current study, we investigated the expression level, biological function and mechanism of circ-LRP6 in ESCC. Circ-LRP6 was sign...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1809922

    authors: Wang J,Zhu W,Tao G,Wang W

    更新日期:2020-12-01 00:00:00

  • Bioengineering advancements, innovations and challenges on green synthesis of 2, 5-furan dicarboxylic acid.

    abstract::The major drawback of chemical transformations for the production of 2, 5-furan dicarboxylic acid (FDCA) implies the usage of hazardous chemicals, high temperature and high pressure from nonrenewable resources. Alternate to chemical methods, biological methods are promising. Microbial FDCA production is improved throu...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2019.1700093

    authors: Rajesh RO,Godan TK,Sindhu R,Pandey A,Binod P

    更新日期:2020-12-01 00:00:00

  • Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes.

    abstract::Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased towa...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2015.1128589

    authors: Bjerga GE,Lale R,Williamson AK

    更新日期:2016-01-01 00:00:00

  • Characteristics of immune cell infiltration and associated diagnostic biomarkers in ulcerative colitis: results from bioinformatics analysis.

    abstract::Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2020.1863016

    authors: Xue G,Hua L,Zhou N,Li J

    更新日期:2021-12-01 00:00:00

  • Potential of Ophiostoma piceae sterol esterase for biotechnologically relevant hydrolysis reactions.

    abstract::The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.22818

    authors: Barba Cedillo V,Prieto A,Martínez MJ

    更新日期:2013-07-01 00:00:00

  • Production optimization of a heat-tolerant alkaline pectinase from Bacillus subtilis ZGL14 and its purification and characterization.

    abstract::Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2017.1292188

    authors: Yu P,Zhang Y,Gu D

    更新日期:2017-09-03 00:00:00

  • Current challenges for the targeted delivery and molecular imaging of stem cells in animal models.

    abstract::In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2016.1233090

    authors: Momeni A,Neelamegham S,Parashurama N

    更新日期:2017-07-04 00:00:00

  • The synthetic biology future.

    abstract::Herein, I track the evolution of synthetic biology from its earliest incarnations more than 50 years ago, through the DIYbio revolution, to the next 50 years. ...

    journal_title:Bioengineered

    pub_type: 社论

    doi:10.4161/bioe.28317

    authors: Sleator RD

    更新日期:2014-03-01 00:00:00

  • Recombinant Lactococcus lactis fails to secrete bovine chymosine.

    abstract::Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for he...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.4161/bioe.36327

    authors: Luerce TD,Azevedo MS,LeBlanc JG,Azevedo V,Miyoshi A,Pontes DS

    更新日期:2014-01-01 00:00:00

  • Enhanced catalytic efficiency of CotA-laccase by DNA shuffling.

    abstract::Bacterial CotA-laccases exhibit higher activity in alkaline pH and salt concentration conditions compared to laccases from white-rot fungi. They are considered as green catalysts in decolorizing of industrial dyes. However, CotA-laccases are limited due to the low yield and catalytic efficiency as the spore-bound natu...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1621134

    authors: Ouyang F,Zhao M

    更新日期:2019-12-01 00:00:00

  • How to achieve high-level expression of microbial enzymes: strategies and perspectives.

    abstract::Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been ...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.4161/bioe.24761

    authors: Liu L,Yang H,Shin HD,Chen RR,Li J,Du G,Chen J

    更新日期:2013-07-01 00:00:00

  • LINC00528 regulates myocardial infarction by targeting the miR-143-3p/COX-2 axis.

    abstract::This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1704535

    authors: Liu K,Zhao D,Wang D

    更新日期:2020-12-01 00:00:00

  • Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes.

    abstract::We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...

    journal_title:Bioengineered

    pub_type: 杂志文章

    doi:10.1080/21655979.2019.1614422

    authors: Huang J,Wang Q,Bu W,Chen L,Yang Z,Zheng W,Li Y,Li J

    更新日期:2019-12-01 00:00:00

  • CRISPR-Cas systems: ushering in the new genome editing era.

    abstract::In recent years there has been great progress with the implementation and utilization of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) systems in the world of genetic engineering. Many forms of CRISPR-Cas9 have been developed as genome editing tools and techniques and...

    journal_title:Bioengineered

    pub_type: 杂志文章,评审

    doi:10.1080/21655979.2018.1470720

    authors: Perez Rojo F,Nyman RKM,Johnson AAT,Navarro MP,Ryan MH,Erskine W,Kaur P

    更新日期:2018-01-01 00:00:00