Characterization of D-xylonate dehydratase YjhG from Escherichia coli.

abstract：:This invited commentary covers the period 1997-2012 and has seen changes in terminology that progressed from "basic" and "applied" to "translational" research. In the context of Bioengineered, these changes map readily onto the processes of identifying microbial characteristics appropriate for specific applications, i...

journal_title：Bioengineered

authors： Dunne C

更新日期：2013-07-01 00:00:00

abstract：:The human chromogranin A-derived peptide CGA-N12, which is composed of 12 amino acid residues with the sequence ALQGAKERAHQQ, showed strong antifungal activity and the least hemolytic activity in previous studies. However, synthetic peptides are relatively expensive to produce. Recombinant expression of peptides in th...

journal_title：Bioengineered

authors： Li X,Fan Y,Lin Q,Luo J,Huang Y,Bao Y,Xu L

更新日期：2020-12-01 00:00:00

abstract：:Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from The Ca...

journal_title：Bioengineered

authors： Cheng YB,Li S,Zhang YP,Zhu ZH,Zhao N

更新日期：2021-01-31 00:00:00

abstract：:Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...

journal_title：Bioengineered

authors： Serpooshan V,Zhao M,Metzler SA,Wei K,Shah PB,Wang A,Mahmoudi M,Malkovskiy AV,Rajadas J,Butte MJ,Bernstein D,Ruiz-Lozano P

更新日期：2014-05-01 00:00:00

abstract：:N-linked glycosylation is a primary source of heterogeneity associated with recombinant monoclonal antibodies and plays a key role in a myriad of drug properties associated with biological function. The glycosylation profile of recombinant monoclonal antibodies is influenced by an array of cell culture inputs which mu...

journal_title：Bioengineered

authors： Schilling M,Feng P,Sosic Z,Traviglia SL

更新日期：2020-12-01 00:00:00

abstract：:This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...

journal_title：Bioengineered

authors： Liu K,Zhao D,Wang D

更新日期：2020-12-01 00:00:00

abstract：:The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone gro...

journal_title：Bioengineered

authors： Yang X,Wang Y,Yan S,Sun L,Yang G,Li Y,Yu C

更新日期：2017-01-02 00:00:00

abstract：:Bacterial CotA-laccases exhibit higher activity in alkaline pH and salt concentration conditions compared to laccases from white-rot fungi. They are considered as green catalysts in decolorizing of industrial dyes. However, CotA-laccases are limited due to the low yield and catalytic efficiency as the spore-bound natu...

journal_title：Bioengineered

authors： Ouyang F,Zhao M

更新日期：2019-12-01 00:00:00

abstract：:The main treatment option for Hemophilia A/B patients involves the administration of recombinant coagulation factors on-demand or in a prophylactic approach. Despite the safety and efficacy of this replacement therapy, the development of antibodies against the coagulation factor infused, which neutralize the procoagul...

journal_title：Bioengineered

authors： Swiech K,Picanço-Castro V,Covas DT

更新日期：2017-09-03 00:00:00

abstract：:We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways ...

journal_title：Bioengineered

authors： Huang J,Wang Q,Bu W,Chen L,Yang Z,Zheng W,Li Y,Li J

更新日期：2019-12-01 00:00:00

abstract：NANNOCHLOROPSIS:sp. is a green alga that is widely used in the aquaculture industry as a feed in Malaysia, but genetic engineering studies of this alga are still underexplored even though there is a growing interest in microalgae genetic engineering for various industrial purposes. This study aims to investigate the ef...

journal_title：Bioengineered

authors： Zainal Abidin AA,Suntarajh M,Balia Yusof ZN

更新日期：2020-12-01 00:00:00

abstract：:This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that ser...

journal_title：Bioengineered

authors： Guo Y

更新日期：2019-12-01 00:00:00

abstract：:The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are 3 examples of natural modular proteins with each ...

journal_title：Bioengineered

authors： Maervoet VET,Briers Y

更新日期：2017-05-04 00:00:00

abstract：:Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...

journal_title：Bioengineered

authors： Xue G,Hua L,Zhou N,Li J

更新日期：2021-12-01 00:00:00

abstract：:In this paper, a miniaturized cell suspension concentration monitoring method was investigated. The sensing unit was a carbon screen-printed electrode (CSPE) in serial with a 433MHz vacuum-packaged surface acoustic wave resonator (SAWR). SAWR provided a stable and high operating frequency, which helps to keep the stab...

journal_title：Bioengineered

authors： Li J,Feng H,Fang Y

更新日期：2015-01-01 00:00:00

abstract：:CRISPR (Clustered Regularly-Interspaced Short Palindromic Repeats)-Cas9 (CRISPR associated protein 9) has rapidly become the most promising genome editing tool with great potential to revolutionize medicine. Through guidance of a 20 nucleotide RNA (gRNA), CRISPR-Cas9 finds and cuts target protospacer DNA precisely 3 b...

journal_title：Bioengineered

authors： Zhang JH,Adikaram P,Pandey M,Genis A,Simonds WF

更新日期：2016-04-01 00:00:00

abstract：:Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...

journal_title：Bioengineered

authors： Welman T,Michel S,Segaren N,Shanmugarajah K

更新日期：2015-01-01 00:00:00

abstract：:Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased towa...

journal_title：Bioengineered

authors： Bjerga GE,Lale R,Williamson AK

更新日期：2016-01-01 00:00:00

abstract：:MiR-25 is a well-documented oncogenic miRNA implicated in esophageal squamous cell carcinoma (ESCC) development, progression and metastasis. However, whether and how miR-25 is involved in the development and metastasis of ESCC remain un-addressed. By using qRT-PCR analysis to compare levels of miR-25 in ESCC tissues w...

journal_title：Bioengineered

authors： Liu B,Li X,Li C,Xu R,Sun X

更新日期：2019-12-01 00:00:00

abstract：:Efficient assembly of multiple DNA fragments is a pivotal technology for synthetic biology. A scarless and sequence-independent DNA assembly method (DATEL) using thermal exonucleases has been developed recently. Here, we present a simplified DATEL (sDATEL) for efficient assembly of unphosphorylated DNA fragments with ...

journal_title：Bioengineered

authors： Ding W,Weng H,Jin P,Du G,Chen J,Kang Z

更新日期：2017-05-04 00:00:00

abstract：:The detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance (MSR) model was studied in this paper. A numerically stimulating model based on MSR was established. And gas-ionizing experiment by adding electronic white noise to induce 1.65 MHz periodic component in the carbon nanotub...

journal_title：Bioengineered

authors： Jingyi Z

更新日期：2015-01-01 00:00:00

abstract：:Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (Ans...

journal_title：Bioengineered

authors： Huerta-Saquero A,Evangelista-Martínez Z,Moreno-Enriquez A,Perez-Rueda E

更新日期：2013-01-01 00:00:00

abstract：:The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...

journal_title：Bioengineered

authors： Wang XM,Liu XM,Wang Y,Chen ZY

更新日期：2021-12-01 00:00:00

abstract：:The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial gr...

journal_title：Bioengineered

authors： Zhou Y,Wang J,Gao X,Wang K,Wang W,Wang Q,Yan P

更新日期：2019-12-01 00:00:00

abstract：:Stromal interaction molecule 1 (STIM1) is a calcium-sensing protein localized in the membrane of the endoplasmic reticulum. The expression of STIM1 has been shown to be closely associated with cell proliferation. The aim of the present study was to investigate the role of STIM1 in the regulation of cancer progression ...

journal_title：Bioengineered

authors： Ge C,Zeng B,Li R,Li Z,Fu Q,Wang W,Wang Z,Dong S,Lai Z,Wang Y,Xue Y,Guo J,Di T,Song X

更新日期：2019-12-01 00:00:00

abstract：:Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker's yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to th...

journal_title：Bioengineered

authors： Oreb M,Dietz H,Farwick A,Boles E

更新日期：2012-11-01 00:00:00

abstract：:Atherosclerosis enables to alter not only the microstructural but also the physical properties of the arterial walls by plaque forming. Few studies so far have been conducted to calculate the isotropic or anisotropic mechanical properties of the healthy and atherosclerotic human coronary arteries. To date there is a p...

journal_title：Bioengineered

authors： Karimi A,Rahmati SM,Sera T,Kudo S,Navidbakhsh M

更新日期：2017-03-04 00:00:00

abstract：:Vaccination is the most cost effective strategy for the control and prevention of the plethora of viral diseases affecting poultry production. The major challenge for poultry vaccination is the design of vaccines that will protect against multiple pathogens via a single protective dose, delivered by mass vaccination. ...

journal_title：Bioengineered

authors： Iqbal M

更新日期：2012-07-01 00:00:00

abstract：:This paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed bypen robot tips under standard conditions. The gene expression ...

journal_title：Bioengineered

authors： Nicolini C,Singh M,Spera R,Felli L

更新日期：2013-09-01 00:00:00

abstract：:A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...

journal_title：Bioengineered

authors： Anis SN,Iqbal NM,Kumar S,Al-Ashraf A

更新日期：2013-03-01 00:00:00