Abstract:
:In this paper, a miniaturized cell suspension concentration monitoring method was investigated. The sensing unit was a carbon screen-printed electrode (CSPE) in serial with a 433MHz vacuum-packaged surface acoustic wave resonator (SAWR). SAWR provided a stable and high operating frequency, which helps to keep the stability and sensitivity of the monitoring system. Living cells suspensions in different concentrations were prepared and dropcast on CSPE. Frequency responses of the sensor were recorded. Cell quantity variation within the same culture media volume changed the dielectric properties of CSPE and finally affected the SAWR frequency. SAWR frequency declined with the decrease of cell concentration. The proposed sensor provided high sensitivity and remarkable stability for the cell suspensions.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Li J,Feng H,Fang Ydoi
10.1080/21655979.2015.1119342subject
Has Abstractpub_date
2015-01-01 00:00:00pages
351-6issue
6eissn
2165-5979issn
2165-5987journal_volume
6pub_type
杂志文章相关文献
Bioengineered文献大全abstract::We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*. ...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.1080/21655979.2016.1171438
更新日期:2016-04-02 00:00:00
abstract::Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.20712
更新日期:2012-09-01 00:00:00
abstract::The bioengineering tools have significant advantages through less time-consuming and utilized as a promising stage for the production of pharmaceutical bioproducts under the single platform. This review highlighted the advantages and current improvement in the plant, animal and microbial bioengineering tools and outli...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2019.1682108
更新日期:2019-12-01 00:00:00
abstract::The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are 3 examples of natural modular proteins with each ...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2016.1222993
更新日期:2017-05-04 00:00:00
abstract::Efficient assembly of multiple DNA fragments is a pivotal technology for synthetic biology. A scarless and sequence-independent DNA assembly method (DATEL) using thermal exonucleases has been developed recently. Here, we present a simplified DATEL (sDATEL) for efficient assembly of unphosphorylated DNA fragments with ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2017.1308986
更新日期:2017-05-04 00:00:00
abstract::Stromal interaction molecule 1 (STIM1) is a calcium-sensing protein localized in the membrane of the endoplasmic reticulum. The expression of STIM1 has been shown to be closely associated with cell proliferation. The aim of the present study was to investigate the role of STIM1 in the regulation of cancer progression ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1669518
更新日期:2019-12-01 00:00:00
abstract::This invited commentary covers the period 1997-2012 and has seen changes in terminology that progressed from "basic" and "applied" to "translational" research. In the context of Bioengineered, these changes map readily onto the processes of identifying microbial characteristics appropriate for specific applications, i...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.23251
更新日期:2013-07-01 00:00:00
abstract::We report the molecular cloning, expression, and single-step homogeneous purification of RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae. RPB5 is a 210 amino acid nuclear protein that functions as the fifth largest subunit of polymerase II and plays a central role in transcription. The gene that codes for...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2014.1002301
更新日期:2015-01-01 00:00:00
abstract::Vaccination is the most cost effective strategy for the control and prevention of the plethora of viral diseases affecting poultry production. The major challenge for poultry vaccination is the design of vaccines that will protect against multiple pathogens via a single protective dose, delivered by mass vaccination. ...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.4161/bioe.20476
更新日期:2012-07-01 00:00:00
abstract::This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that ser...
journal_title:Bioengineered
pub_type: 杂志文章,收录出版
doi:10.1080/21655979.2019.1652490
更新日期:2019-12-01 00:00:00
abstract::Prediction of tissue damage under thermal loads plays important role for thermal ablation planning. A new methodology is presented in this paper by combing non-Fourier bio-heat transfer, constitutive elastic mechanics as well as non-rigid motion of dynamics to predict and analyze thermal distribution, thermal-induced ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1227609
更新日期:2017-01-02 00:00:00
abstract::Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2015.1081320
更新日期:2015-01-01 00:00:00
abstract::We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also r...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.27607
更新日期:2014-05-01 00:00:00
abstract::Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.27751
更新日期:2014-05-01 00:00:00
abstract::Bacterial CotA-laccases exhibit higher activity in alkaline pH and salt concentration conditions compared to laccases from white-rot fungi. They are considered as green catalysts in decolorizing of industrial dyes. However, CotA-laccases are limited due to the low yield and catalytic efficiency as the spore-bound natu...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1621134
更新日期:2019-12-01 00:00:00
abstract::A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22350
更新日期:2013-03-01 00:00:00
abstract::Circular RNA (circRNA) circ-LRP6 was recently proven to be a pivotal player in various human diseases. Nevertheless, its role in esophageal squamous cell cancer (ESCC) remains unknown. In the current study, we investigated the expression level, biological function and mechanism of circ-LRP6 in ESCC. Circ-LRP6 was sign...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1809922
更新日期:2020-12-01 00:00:00
abstract::Ulcerative colitis (UC) is a type of refractory and recurrent inflammatory disorder that occurs in colon and rectum. Immune cell infiltration plays a critical role in UC progression; therefore, this study aims to explore potential biomarkers for UC and to analyze characteristics of immune cell infiltration based on th...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1863016
更新日期:2021-12-01 00:00:00
abstract::Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been ...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.24761
更新日期:2013-07-01 00:00:00
abstract::The successful treatment of keloids is a great challenge in the plastic surgery field. Activating transcription factor 3 (ATF3) is discovered as an adaptive responsive gene, which plays a critical role in fibroblast activation. This study aimed to investigate the expression and biological role of ATF3 in the pathogene...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1860491
更新日期:2021-12-01 00:00:00
abstract::This study is aimed to explore the roles of LINC00528 in myocardial infarction (MI) progression. Quantitative real-time PCR showed that the expression of LINC00528 and COX-2 was upregulated while miR-143-3p expression was down-regulated in post-MI cells. In function assays, LINC00528 suppression promoted post-MI cells...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2019.1704535
更新日期:2020-12-01 00:00:00
abstract::Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (Ans...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.21710
更新日期:2013-01-01 00:00:00
abstract::Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.28791
更新日期:2014-07-01 00:00:00
abstract::The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22818
更新日期:2013-07-01 00:00:00
abstract::Atherosclerosis enables to alter not only the microstructural but also the physical properties of the arterial walls by plaque forming. Few studies so far have been conducted to calculate the isotropic or anisotropic mechanical properties of the healthy and atherosclerotic human coronary arteries. To date there is a p...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1212134
更新日期:2017-03-04 00:00:00
abstract::The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in nor...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1227143
更新日期:2016-09-02 00:00:00
abstract::In this study, a total of six fungal samples were isolated from apple, strawberry and orange pulp. DNA sequence analysis was used as molecular identification method. ITS region was aligned in DNA sequence analysis, and an algorithm sequence similarity was done using BLAST (Basic Local Alignment Search Tool) program to...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1739379
更新日期:2020-12-01 00:00:00
abstract::Alkaline pectinase has important applications in the pretreatment of waste water from food processing and in both the fabric and paper industries. In this study, a 2-level factorial design was used to screen significant factors that affect the activity of alkaline pectinase, and the response surface methodology (RSM) ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2017.1292188
更新日期:2017-09-03 00:00:00
abstract::Rhizoremediation is one of the most accepted, cost-effective bioremediation techniques focusing on the application of rhizospheric microorganisms in combination with plants for the remediation of organic and inorganic pollutants from the contaminated sites. This work focuses on isolation and identification of metal re...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2020.1728034
更新日期:2020-12-01 00:00:00
abstract::The major drawback of chemical transformations for the production of 2, 5-furan dicarboxylic acid (FDCA) implies the usage of hazardous chemicals, high temperature and high pressure from nonrenewable resources. Alternate to chemical methods, biological methods are promising. Microbial FDCA production is improved throu...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2019.1700093
更新日期:2020-12-01 00:00:00