Exploring processing adjuvants' influence on traditional Chinese medicine compatibility of Aconiti Radix Cocta and Pinelliae rhizome using rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry.

Abstract:

BACKGROUND:It is known that when crude Pinelliae rhizome and Pinelliae rhizoma preparatum are combined with Aconiti Radix Cocta respectively, the toxicity of the combination varies. However, the component's transformation between different compatibility have remained unclear. OBJECTIVE:In this paper, a novel approach using rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry (RRLC-Q-TOF-MS) coupled with multivariate statistical analysis was established for exploring the influence of processing adjuvants (PAs) on the compatibility of Aconiti Radix Cocta and Pinelliae rhizome. MATERIALS AND METHODS:In order to obtain information about the representative markers between different groups, an exhaustive study of different protocols based on adding or removing different PAs step by step was carried out and the influence of PAs on compatibility was investigated. RESULTS:It was found that lime can facilitate diester diterpenoid alkaloids with high toxicity in Aconiti Radix Cocta to be converted into low-toxic or non-toxic derivatives. Glycyrrhizae Radix et Rhizoma had no remarkable effect on the process. CONCLUSION:The established method in this study will be of great significance to process research mechanism and study on traditional Chinese Medicine compatibility and clinical application.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Wang Y,Li Y,Zhang X,Xu Y,Wang H,Zhang Y

doi

10.4103/0973-1296.141771

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

483-90

issue

40

eissn

0973-1296

issn

0976-4062

pii

PM-10-483

journal_volume

10

pub_type

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