Abstract:
BACKGROUND:Hakea sericea Schrader is an invasive shrub in Portuguese forests. OBJECTIVE:The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of these extracts, against human cells, were assessed. MATERIALS AND METHODS:The antimicrobial activity of the methanolic extracts of H. sericea was assessed by disk diffusion assay and Minimum Inhibitory Concentration (MIC) value determination. The antibiofilm activity was determined by quantification of total biofilm biomass with crystal violet. Cytotoxicity was evaluated by hemolysis assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. RESULTS:For Gram-positive bacteria, MIC values of H. sericea methanolic extracts ranged between 0.040 and 0.625 mg/mL, whereas the fruits extract yielded the lowest MIC for several strains of microorganisms, namely, S. aureus, B. cereus, L. monocytogenes and clinical methicillin-resistant S. aureus (MRSA). Stems and fruits extract at 2.5 mg/mL effectively eradicated the biofilm of S. aureus ATCC 25923, SA 01/10 and MRSA 12/10. Regarding leaves extract, hemolysis was not observed, and in the case of stems and fruits, hemolysis was verified only for higher concentrations, suggesting its low toxicity. Fruits extract presented no toxic effect to normal human dermal fibroblasts (NHDF) cells however for concentrations of 0.017 and 0.008 mg/mL this extract was able to decrease human breast adenocarcinoma cells (MCF-7) viability in about 60%, as MTT test results had confirmed. This is a clearly demonstrator of the cytotoxicity of this extract against MCF-7 cells.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Luís A,Breitenfeld L,Ferreira S,Duarte AP,Domingues Fdoi
10.4103/0973-1296.127331subject
Has Abstractpub_date
2014-01-01 00:00:00pages
S6-S13issue
Suppl 1eissn
0973-1296issn
0976-4062pii
PM-10-6journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Polygonum multiflorum is an important medicinal plant. Hairy roots systems obtained by transforming plant tissues with the natural genetic engineer Agrobacterium rhizogenes can produce valuable biological active substances, which have immense potential in the pharmaceutical industry. OBJECTIVE:To optimize t...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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journal_title:Pharmacognosy magazine
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更新日期:2014-08-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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journal_title:Pharmacognosy magazine
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更新日期:2014-08-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2013-04-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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更新日期:2014-10-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2016-05-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2016-05-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.153063
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.186347
更新日期:2016-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.166018
更新日期:2015-10-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.204555
更新日期:2017-04-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2016-05-01 00:00:00