Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium.

Abstract:

BACKGROUND:Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. MATERIALS AND METHODS:The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied through a single-factor experiment. An orthogonal array (L9(3(4))) was then constructed to achieve the best extraction conditions. RESULTS:Variance analysis revealed that ethanol concentration significantly affected the extraction yield. The optimal conditions were as follows: ethanol concentration, 50% (v/v); liquid to solid ratio, 15:1; duration of microwave irradiation, 240 s; and extraction temperature, 60°C. CONCLUSION:Under these optimal conditions, the total flavonoid yield was 18.34 ± 0.64 mg/g. The use of a microwave-assisted process dramatically reduced the time needed for extraction of flavonoids from I. helenium.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Guo CY,Wang J,Hou Y,Zhao YM,Shen LX,Zhang DS

doi

10.4103/0973-1296.113260

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

192-5

issue

35

eissn

0973-1296

issn

0976-4062

pii

PM-9-192

journal_volume

9

pub_type

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