Abstract:
BACKGROUND:Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. MATERIALS AND METHODS:The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied through a single-factor experiment. An orthogonal array (L9(3(4))) was then constructed to achieve the best extraction conditions. RESULTS:Variance analysis revealed that ethanol concentration significantly affected the extraction yield. The optimal conditions were as follows: ethanol concentration, 50% (v/v); liquid to solid ratio, 15:1; duration of microwave irradiation, 240 s; and extraction temperature, 60°C. CONCLUSION:Under these optimal conditions, the total flavonoid yield was 18.34 ± 0.64 mg/g. The use of a microwave-assisted process dramatically reduced the time needed for extraction of flavonoids from I. helenium.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Guo CY,Wang J,Hou Y,Zhao YM,Shen LX,Zhang DSdoi
10.4103/0973-1296.113260subject
Has Abstractpub_date
2013-07-01 00:00:00pages
192-5issue
35eissn
0973-1296issn
0976-4062pii
PM-9-192journal_volume
9pub_type
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