Abstract:
BACKGROUND:The RP-HPLC resolution of two triterpenic acid isomers was unstable. OBJECTIVE:To separate the oleanolic acid (OA) and ursolic acid (UA) simply within RP-HPLC. MATERIALS AND METHODS:The separation ability of five stationary phases was studied with the retention effect of their carbon loads. Also the resolution effects of mobile phase composition and different column temperatures were systematically investigated by using Drylab(®) (Rheodyne LLC.) after evaluating chromatograms automatically. RESULTS:The best available resolution of two bioactive isomers was achieved (r = 3.4) via using PAH (polycyclic aromatic hydrocarbons) polymeric C18 bonded phase column. The chromatographic system was applied to the quantification in ten Chinese medicinal plants and the validation was carried out and the precision (RSD ≤1.34%), the linearity (r ≥ 0.9998) and the recovery (range from 92.1% to 102.6%) were acceptable. CONCLUSION:It is clear that the method was simple, rapid and reliable for the quantification of two compounds in new HPLC method within PAH polymeric C18.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Zhang Y,Xue K,Zhao EY,Li Y,Yao L,Yang X,Xie Xdoi
10.4103/0973-1296.117853subject
Has Abstractpub_date
2013-10-01 00:00:00pages
S19-24issue
Suppl 1eissn
0973-1296issn
0976-4062pii
PM-9-19journal_volume
9pub_type
杂志文章abstract:Background:Academic reports have confirmed Moringa oleifera leaves to possess significant antioxidant capacities; however, such studies are unavailable for its ripe seeds even though they are more desirous for consumption due to their sweet taste. Objective:In this study, we investigated antioxidant capacities of four...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_212_17
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Abelmoschus esculentus L. is a healthy vegetable belonging to the family Malvaceae. This article reports the contents of total phenolics (TP) and total flavonoids (TF) in 80% methanol extracts of the flower (FL), fruit (FR), leaf (L), and seed (S) of A. esculentus, and in 0, 10, 30, 50, and 70% methanol elua...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.96570
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:The androgen comprises a group of hormones that play roles in male reproductive activity as well as personal characteristics. OBJECTIVE:We investigated the androgenic activity of various herbal medicines in human prostate cancer 22Rv1 cells. MATERIALS AND METHODS:Herbal extracts of Trichosanthes kirilowii ...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.197657
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:The majority of Achillea species are the most important native economic plants of Anatolia. They include highly bioactive compounds, so they have therapeutic applications. OBJECTIVE:In the present study, the aim was to investigate in vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea tereti...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.166060
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Blechnum orientale Linn. (Blechnaceae), a fern, is traditionally used in the treatment of various ailments, such as skin diseases, stomach pain, urinary bladder complaints, and also as a female contraceptive. Previously, we reported a strong radical scavenging activity, antibacterial activity and cytotoxicit...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.197659
更新日期:2017-01-01 00:00:00
abstract:CONTEXT:Scyphiphora hydrophyllacea is a shrub mangrove plant of the family Rubiaceae and not yet been studied for anti-hepatocarcinogenic effects. OBJECTIVES:We investigated possible in vitro anti-hepatocarcinogenic and antioxidant properties of S. hydrophyllacea. MATERIALS AND METHODS:Dried leaves of S. hydrophyllac...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.203989
更新日期:2017-01-01 00:00:00
abstract:Background:Chemical fungicides are widely used to control crop diseases, but these chemicals have adverse effects. They destroy the ecological environment and even have toxic effects on human beings. In this context, the development of botanical pesticides is relevant. One potential botanical pesticide is chelerythrine...
journal_title:Pharmacognosy magazine
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更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Radix Saposhnikoviae (RS) exerts anti-inflammatory, analgesic, antipyretic, antioxidation effects and has been used in traditional Chinese medicine to treat common colds, headache, and rheumatoid arthritis. Prim-O-glucosylcimifugin (POG) is the highest content chromone and one of the major active constituent...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Traditionally, a number of medicinal plants are used to treat various types of hepatic disorders but few of them were pharmacologically evaluated for their safety and efficacy. The combination of Andrographis paniculata (Kalmegha), Tinospora cordifolia (Guduchi), and Solanum nigrum (Kakmachi) was traditional...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.168945
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Occurrence of integristerone A (1), 20-hydroxyecdysone (2), ecdysone (3), 2-deoxy-20-hydroxyecdysone (4) has been analyzed in 64 species of the East Asian Caryophyllaceae. MATERIALS AND METHODS:Ecdysteroid content was determinate by high-performance liquid chromatography (HPLC). HPLC with a high-resolution ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.157746
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:Hakea sericea Schrader is an invasive shrub in Portuguese forests. OBJECTIVE:The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.127331
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Haberlea rhodopensis Friv. (Gesneriaceae) is a rare poikilohydric endemic and preglacial relict growing in Balkan Peninsula. Previous investigations demonstrated strong antioxidant, antimicrobial and antimutagenic potential of alcoholic extract from the plant. OBJECTIVE:The isolation of known caffeoyl pheny...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.117822
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Cyperus scariosus R. Br and Cyperus rotundus L are widely used in ayurvedic preparation for the treatment of diabetes and other diseases. The early literature, so far, does not indicate the presence of any bioactive principle isolated from these plants. OBJECTIVE:To identify free radical scavenging, anti-di...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.191467
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:The active ingredients and thus pharmacological efficacy of traditional Chinese medicine (TCM) at different degrees of parching process vary greatly. OBJECTIVE:Near-infrared spectroscopy (NIR) was used to develop a new method for rapid online analysis of TCM parching process, using two kinds of chemical ind...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_416_16
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:Tithonia diversifolia belongs to the Asteraceae family. The leaves of T. diversifolia have been studied lately because of the presence of tagitinin C. OBJECTIVE:Looking for an easy and inexpensive method to extract tagitinin C from T. diversifolia leaves, this work aims to conduct a screening to evaluate th...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.204555
更新日期:2017-04-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.149735
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Yinqiaosan (Lonicerae and Forsythiae Powder), as a famous prescription of Dr. Wu Jutong in Qing dynasty of China, has the effects of diaphoresis cooling, fire-purging, and detoxicaton. It is mainly used in the treatment of influenza, hand-foot-mouth disease, esophagitis, pneumonia, acute tonsillitis, mumps, ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.177911
更新日期:2016-04-01 00:00:00
abstract:Background:Meyna spinosa (M.S) (Roxb.) ex Link and Oroxylum indicum (O.I) (Linn.) Vent, widely used traditional Northeast Indian medicinal plant used for various purposes, have not yet explored for safety profile. Objective:To investigate the safety profile of M.S (Roxb.) ex Link leaves and O.I (Linn.) Vent stem bark ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
abstract::Mentha piperita essential oil was bactericidal in order of E. coli> S. aureus > Pseudomonas aeruginosa > S. faecalis > Klebsiella pneumoniae. The oil with total phenolics of 89.43 ± 0.58 µg GAE/mg had 63.82 ± 0.05% DPPH inhibition activity with an IC (50) = 3.9 µg/ml. Lipid peroxidation inhibition was comparable to BH...
journal_title:Pharmacognosy magazine
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更新日期:2010-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. MATERIALS AND METHODS:The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied th...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. OBJECTIVE:A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- h...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.133267
更新日期:2014-04-01 00:00:00
abstract:Objective:Wine grape sap or bleeding sap of grapes (GBS) is commonly used in Xinjiang (China) for therapeutic aims. Do variations in composition related to region and variety affect its properties? Methods:GBS samples originating in various parts of Xinjiang (Turpan, Hotan, Kashgar, and Atush) were tested for phenols ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_358_16
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Rhododendron is a group of famous landscape plants with high medicinal value. However, there is no simple or universal manner to discriminate the various species of this group. Deoxyribonucleic acid (DNA) barcoding technique is a new biological tool that can accurately and objectively identify species by usi...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.93311
更新日期:2012-01-01 00:00:00
abstract:CONTEXT:Although many studies displayed the favorable effects of Assafoetida, some of them reported that high doses of Assafoetida could lead to harmful effects. AIMS:In this study, the effect of pretreatment with Assafoetida investigated on ischemic-reperfusion injury in isolated rat heart model. MATERIALS AND METHO...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.131026
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:Rhizoma dioscoreae nipponicae is a perennial herb and its roots have been widely used in Traditional Chinese Medicine (TCM). OBJECTIVE:To develop and optimize the extraction and hydrolysis technology of diosgenin from Rhizoma dioscoreae nipponicae. MATERIALS AND METHODS:1-methyl-3-(3-sulfopropyl)-imidazoli...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Coffea canephora is the commonly cultivated coffee species in the world along with Coffea arabica. Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particul...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_518_16
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Plant sterols are the major source of micronutrients and have not shown any obvious side effects in human. β-sitosterol is one of the most prevalent phytosterols which have been recorded in ancient medicinal history for its use in the treatment of many chronic diseases, especially cancer. The modulations of ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.197634
更新日期:2017-01-01 00:00:00
abstract:Background:Moringa oleifera (MO), commonly known as the drumstick tree, is used in folklore medicine for the treatment of skin disease. Objective:The objective of this study is to evaluate the ethyl acetate (EtOAc) fraction of MO leaves for in vitro antibacterial, antioxidant, and wound healing activities and conduct ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_368_16
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:The present study examined the pharmacokinetic profiles of two iridoid glycosides named morroniside and loganin in rat plasma after oral administration of crude and processed Cornus officinals. MATERIALS AND METHODS:A rapid, selective and specific high-performance liquid chromatography/electrospray ionizatio...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.127358
更新日期:2014-01-01 00:00:00