Determination of oleanolic acid and ursolic acid in Chinese medicinal plants using HPLC with PAH polymeric C18.

Abstract:

BACKGROUND:The RP-HPLC resolution of two triterpenic acid isomers was unstable. OBJECTIVE:To separate the oleanolic acid (OA) and ursolic acid (UA) simply within RP-HPLC. MATERIALS AND METHODS:The separation ability of five stationary phases was studied with the retention effect of their carbon loads. Also the resolution effects of mobile phase composition and different column temperatures were systematically investigated by using Drylab(®) (Rheodyne LLC.) after evaluating chromatograms automatically. RESULTS:The best available resolution of two bioactive isomers was achieved (r = 3.4) via using PAH (polycyclic aromatic hydrocarbons) polymeric C18 bonded phase column. The chromatographic system was applied to the quantification in ten Chinese medicinal plants and the validation was carried out and the precision (RSD ≤1.34%), the linearity (r ≥ 0.9998) and the recovery (range from 92.1% to 102.6%) were acceptable. CONCLUSION:It is clear that the method was simple, rapid and reliable for the quantification of two compounds in new HPLC method within PAH polymeric C18.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Zhang Y,Xue K,Zhao EY,Li Y,Yao L,Yang X,Xie X

doi

10.4103/0973-1296.117853

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

S19-24

issue

Suppl 1

eissn

0973-1296

issn

0976-4062

pii

PM-9-19

journal_volume

9

pub_type

杂志文章
  • Total Phenolics, Total Flavonoids, Antioxidant Capacities, and Volatile Compounds Gas Chromatography-Mass Spectrometry Profiling of Moringa oleifera Ripe Seed Polar Fractions.

    abstract:Background:Academic reports have confirmed Moringa oleifera leaves to possess significant antioxidant capacities; however, such studies are unavailable for its ripe seeds even though they are more desirous for consumption due to their sweet taste. Objective:In this study, we investigated antioxidant capacities of four...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_212_17

    authors: Adebayo IA,Arsad H,Samian MR

    更新日期:2018-04-01 00:00:00

  • Analysis and comparison of the active components and antioxidant activities of extracts from Abelmoschus esculentus L.

    abstract:BACKGROUND:Abelmoschus esculentus L. is a healthy vegetable belonging to the family Malvaceae. This article reports the contents of total phenolics (TP) and total flavonoids (TF) in 80% methanol extracts of the flower (FL), fruit (FR), leaf (L), and seed (S) of A. esculentus, and in 0, 10, 30, 50, and 70% methanol elua...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.96570

    authors: Liao H,Dong W,Shi X,Liu H,Yuan K

    更新日期:2012-04-01 00:00:00

  • Trichosanthes kirilowii Exerts Androgenic Activity via Regulation of PSA and KLK2 in 22Rv1 Prostate Cancer Cells.

    abstract:BACKGROUND:The androgen comprises a group of hormones that play roles in male reproductive activity as well as personal characteristics. OBJECTIVE:We investigated the androgenic activity of various herbal medicines in human prostate cancer 22Rv1 cells. MATERIALS AND METHODS:Herbal extracts of Trichosanthes kirilowii ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.197657

    authors: Jeong SJ,Choi JY,Dong MS,Seo CS,Shin HK

    更新日期:2017-01-01 00:00:00

  • In vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea teretifolia Willd extracts on human prostate cancer cell lines.

    abstract:BACKGROUND:The majority of Achillea species are the most important native economic plants of Anatolia. They include highly bioactive compounds, so they have therapeutic applications. OBJECTIVE:In the present study, the aim was to investigate in vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea tereti...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.166060

    authors: Bali EB,Açık L,Elçi P,Sarper M,Avcu F,Vural M

    更新日期:2015-10-01 00:00:00

  • Isolation and Characterisation of a Proanthocyanidin With Antioxidative, Antibacterial and Anti-Cancer Properties from Fern Blechnum orientale.

    abstract:BACKGROUND:Blechnum orientale Linn. (Blechnaceae), a fern, is traditionally used in the treatment of various ailments, such as skin diseases, stomach pain, urinary bladder complaints, and also as a female contraceptive. Previously, we reported a strong radical scavenging activity, antibacterial activity and cytotoxicit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.197659

    authors: Lai HY,Lim YY,Kim KH

    更新日期:2017-01-01 00:00:00

  • Anti-hepatocarcinogenic and Anti-oxidant Effects of Mangrove Plant Scyphiphora hydrophyllacea.

    abstract:CONTEXT:Scyphiphora hydrophyllacea is a shrub mangrove plant of the family Rubiaceae and not yet been studied for anti-hepatocarcinogenic effects. OBJECTIVES:We investigated possible in vitro anti-hepatocarcinogenic and antioxidant properties of S. hydrophyllacea. MATERIALS AND METHODS:Dried leaves of S. hydrophyllac...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.203989

    authors: Samarakoon SR,Shanmuganathan C,Ediriweera MK,Piyathilaka P,Tennekoon KH,Thabrew I,Galhena P,De Silva ED

    更新日期:2017-01-01 00:00:00

  • Extraction of Chelerythrine and its Effects on Pathogenic Fungus Spore Germination.

    abstract:Background:Chemical fungicides are widely used to control crop diseases, but these chemicals have adverse effects. They destroy the ecological environment and even have toxic effects on human beings. In this context, the development of botanical pesticides is relevant. One potential botanical pesticide is chelerythrine...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_545_16

    authors: Wei Q,Zhao M,Li X

    更新日期:2017-10-01 00:00:00

  • Prim-O-glucosylcimifugin Attenuates Lipopolysaccharideinduced Inflammatory Response in RAW 264.7 Macrophages.

    abstract:BACKGROUND:Radix Saposhnikoviae (RS) exerts anti-inflammatory, analgesic, antipyretic, antioxidation effects and has been used in traditional Chinese medicine to treat common colds, headache, and rheumatoid arthritis. Prim-O-glucosylcimifugin (POG) is the highest content chromone and one of the major active constituent...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_323_16

    authors: Zhou J,Sun YY,Sun MY,Mao WA,Wang L,Zhang J,Zhang H

    更新日期:2017-07-01 00:00:00

  • Hepatoprotective Effect of A Polyherbal Extract Containing Andrographis Paniculata, Tinospora Cordifolia and Solanum Nigrum Against Paracetamol Induced Hepatotoxicity.

    abstract:BACKGROUND:Traditionally, a number of medicinal plants are used to treat various types of hepatic disorders but few of them were pharmacologically evaluated for their safety and efficacy. The combination of Andrographis paniculata (Kalmegha), Tinospora cordifolia (Guduchi), and Solanum nigrum (Kakmachi) was traditional...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.168945

    authors: Singh DP,Awasthi H,Luqman S,Singh S,Mani D

    更新日期:2015-10-01 00:00:00

  • Phytoecdysteroids of the East Asian Caryophyllaceae.

    abstract:BACKGROUND:Occurrence of integristerone A (1), 20-hydroxyecdysone (2), ecdysone (3), 2-deoxy-20-hydroxyecdysone (4) has been analyzed in 64 species of the East Asian Caryophyllaceae. MATERIALS AND METHODS:Ecdysteroid content was determinate by high-performance liquid chromatography (HPLC). HPLC with a high-resolution ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.157746

    authors: Novozhilova E,Rybin V,Gorovoy P,Gavrilenko I,Doudkin R

    更新日期:2015-05-01 00:00:00

  • Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts.

    abstract:BACKGROUND:Hakea sericea Schrader is an invasive shrub in Portuguese forests. OBJECTIVE:The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.127331

    authors: Luís A,Breitenfeld L,Ferreira S,Duarte AP,Domingues F

    更新日期:2014-01-01 00:00:00

  • Cytoprotective and antioxidant effects of phenolic compounds from Haberlea rhodopensis Friv. (Gesneriaceae).

    abstract:BACKGROUND:Haberlea rhodopensis Friv. (Gesneriaceae) is a rare poikilohydric endemic and preglacial relict growing in Balkan Peninsula. Previous investigations demonstrated strong antioxidant, antimicrobial and antimutagenic potential of alcoholic extract from the plant. OBJECTIVE:The isolation of known caffeoyl pheny...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.117822

    authors: Kondeva-Burdina M,Zheleva-Dimitrova D,Nedialkov P,Girreser U,Mitcheva M

    更新日期:2013-10-01 00:00:00

  • Free radical scavenging, α-glucosidase inhibitory and anti-inflammatory constituents from Indian sedges, Cyperus scariosus R.Br and Cyperus rotundus L.

    abstract:BACKGROUND:Cyperus scariosus R. Br and Cyperus rotundus L are widely used in ayurvedic preparation for the treatment of diabetes and other diseases. The early literature, so far, does not indicate the presence of any bioactive principle isolated from these plants. OBJECTIVE:To identify free radical scavenging, anti-di...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.191467

    authors: Kakarla L,Katragadda SB,Tiwari AK,Kotamraju KS,Madhusudana K,Kumar DA,Botlagunta M

    更新日期:2016-07-01 00:00:00

  • Near-infrared Spectroscopy as a Process Analytical Technology Tool for Monitoring the Parching Process of Traditional Chinese Medicine Based on Two Kinds of Chemical Indicators.

    abstract:BACKGROUND:The active ingredients and thus pharmacological efficacy of traditional Chinese medicine (TCM) at different degrees of parching process vary greatly. OBJECTIVE:Near-infrared spectroscopy (NIR) was used to develop a new method for rapid online analysis of TCM parching process, using two kinds of chemical ind...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_416_16

    authors: Li K,Wang W,Liu Y,Jiang S,Huang G,Ye L

    更新日期:2017-04-01 00:00:00

  • Comparison of Ultrasound-assisted Extraction and Dynamic Maceration Over Content of Tagitinin C obtained from Tithonia diversifolia (Hemsl.) A. Gray Leaves Using Factorial Design.

    abstract:BACKGROUND:Tithonia diversifolia belongs to the Asteraceae family. The leaves of T. diversifolia have been studied lately because of the presence of tagitinin C. OBJECTIVE:Looking for an easy and inexpensive method to extract tagitinin C from T. diversifolia leaves, this work aims to conduct a screening to evaluate th...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.204555

    authors: Silva AMR,Ferreira NLO,Oliveira AE,Borges LL,Conceição EC

    更新日期:2017-04-01 00:00:00

  • Chromatographic method for determination of the free amino acid content of chamomile flowers.

    abstract:OBJECTIVE:To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. MATERIALS AND METHODS:Derivatization wit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.149735

    authors: Ma X,Zhao D,Li X,Meng L

    更新日期:2015-01-01 00:00:00

  • A Study on the Chemical Compositions of the Yinqiaosan (Lonicerae and Forsythiae Powder) at Different Time of Later-decoction by Gas Chromatography Mass Spectrometry.

    abstract:BACKGROUND:Yinqiaosan (Lonicerae and Forsythiae Powder), as a famous prescription of Dr. Wu Jutong in Qing dynasty of China, has the effects of diaphoresis cooling, fire-purging, and detoxicaton. It is mainly used in the treatment of influenza, hand-foot-mouth disease, esophagitis, pneumonia, acute tonsillitis, mumps, ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.177911

    authors: Shu Y,Chen Y,Qin K,Liu X,Cai B

    更新日期:2016-04-01 00:00:00

  • Safety Profile Investigations of Meyna spinosa (Roxb.) and Oroxylum indicum (Linn.) Extracts Collected from Northeast India.

    abstract:Background:Meyna spinosa (M.S) (Roxb.) ex Link and Oroxylum indicum (O.I) (Linn.) Vent, widely used traditional Northeast Indian medicinal plant used for various purposes, have not yet explored for safety profile. Objective:To investigate the safety profile of M.S (Roxb.) ex Link leaves and O.I (Linn.) Vent stem bark ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_62_15

    authors: Singh S,Chattopadhyay P,Borthakur SK,Policegoudra R

    更新日期:2018-01-01 00:00:00

  • Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials.

    abstract::Mentha piperita essential oil was bactericidal in order of E. coli> S. aureus > Pseudomonas aeruginosa > S. faecalis > Klebsiella pneumoniae. The oil with total phenolics of 89.43 ± 0.58 µg GAE/mg had 63.82 ± 0.05% DPPH inhibition activity with an IC (50) = 3.9 µg/ml. Lipid peroxidation inhibition was comparable to BH...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.66926

    authors: Sharafi SM,Rasooli I,Owlia P,Taghizadeh M,Astaneh SD

    更新日期:2010-07-01 00:00:00

  • Liquid Chromatography Mass Spectrometry Analysis and Cytotoxicity of Asparagus adscendens Roots against Human Cancer Cell Lines.

    abstract:Background:Asparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. This plant has been used in the prevention and effective treatment of various forms of cancers. Objective:This paper reports, for the first time, on the cytotoxicity of the methanol (MeOH) extract of the roots of A. adscendens and its so...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_136_17

    authors: Khan KM,Nahar L,Mannan A,Arfan M,Khan GA,Al-Groshi A,Evans A,Dempster NM,Ismail FMD,Sarker SD

    更新日期:2018-01-01 00:00:00

  • Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium.

    abstract:BACKGROUND:Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. MATERIALS AND METHODS:The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied th...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.113260

    authors: Guo CY,Wang J,Hou Y,Zhao YM,Shen LX,Zhang DS

    更新日期:2013-07-01 00:00:00

  • Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection.

    abstract:BACKGROUND:Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. OBJECTIVE:A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- h...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.133267

    authors: Yun BR,Weon JB,Lee J,Eom MR,Ma CJ

    更新日期:2014-04-01 00:00:00

  • Constituents and Antioxidant Activity of Bleeding Sap from Various Xinjiang Grapes.

    abstract:Objective:Wine grape sap or bleeding sap of grapes (GBS) is commonly used in Xinjiang (China) for therapeutic aims. Do variations in composition related to region and variety affect its properties? Methods:GBS samples originating in various parts of Xinjiang (Turpan, Hotan, Kashgar, and Atush) were tested for phenols ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_358_16

    authors: Le L,Umar A,Iburaim A,Moore N

    更新日期:2017-10-01 00:00:00

  • Species identification of Rhododendron (Ericaceae) using the chloroplast deoxyribonucleic acid PsbA-trnH genetic marker.

    abstract:BACKGROUND:Rhododendron is a group of famous landscape plants with high medicinal value. However, there is no simple or universal manner to discriminate the various species of this group. Deoxyribonucleic acid (DNA) barcoding technique is a new biological tool that can accurately and objectively identify species by usi...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.93311

    authors: Liu Y,Zhang L,Liu Z,Luo K,Chen S,Chen K

    更新日期:2012-01-01 00:00:00

  • Pretreatment with Assafoetida exerts dose-dependent dual effects on rat hearts.

    abstract:CONTEXT:Although many studies displayed the favorable effects of Assafoetida, some of them reported that high doses of Assafoetida could lead to harmful effects. AIMS:In this study, the effect of pretreatment with Assafoetida investigated on ischemic-reperfusion injury in isolated rat heart model. MATERIALS AND METHO...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.131026

    authors: Esmailidehaj M,Kakoo M,Rezvani ME,Mosaddeghmehrjardi MH

    更新日期:2014-04-01 00:00:00

  • New ionic liquid-based preparative method for diosgenin from Rhizoma dioscoreae nipponicae.

    abstract:BACKGROUND:Rhizoma dioscoreae nipponicae is a perennial herb and its roots have been widely used in Traditional Chinese Medicine (TCM). OBJECTIVE:To develop and optimize the extraction and hydrolysis technology of diosgenin from Rhizoma dioscoreae nipponicae. MATERIALS AND METHODS:1-methyl-3-(3-sulfopropyl)-imidazoli...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.113282

    authors: Yan W,Ji L,Hang S,Shun Y

    更新日期:2013-07-01 00:00:00

  • Identification and Analysis of Jasmonate Pathway Genes in Coffea canephora (Robusta Coffee) by In Silico Approach.

    abstract:BACKGROUND:Coffea canephora is the commonly cultivated coffee species in the world along with Coffea arabica. Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particul...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_518_16

    authors: Bharathi K,Sreenath HL

    更新日期:2017-07-01 00:00:00

  • Evaluate the Antigenotoxicity and Anticancer Role of β-Sitosterol by Determining Oxidative DNA Damage and the Expression of Phosphorylated Mitogen-activated Protein Kinases', C-fos, C-jun, and Endothelial Growth Factor Receptor.

    abstract:BACKGROUND:Plant sterols are the major source of micronutrients and have not shown any obvious side effects in human. β-sitosterol is one of the most prevalent phytosterols which have been recorded in ancient medicinal history for its use in the treatment of many chronic diseases, especially cancer. The modulations of ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.197634

    authors: Sharmila R,Sindhu G

    更新日期:2017-01-01 00:00:00

  • Chemical Composition of Moringa oleifera Ethyl Acetate Fraction and Its Biological Activity in Diabetic Human Dermal Fibroblasts.

    abstract:Background:Moringa oleifera (MO), commonly known as the drumstick tree, is used in folklore medicine for the treatment of skin disease. Objective:The objective of this study is to evaluate the ethyl acetate (EtOAc) fraction of MO leaves for in vitro antibacterial, antioxidant, and wound healing activities and conduct ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_368_16

    authors: Gothai S,Muniandy K,Zarin MA,Sean TW,Kumar SS,Munusamy MA,Fakurazi S,Arulselvan P

    更新日期:2017-10-01 00:00:00

  • Comparison of pharmacokinetic behavior of two iridoid glycosides in rat plasma after oral administration of crude Cornus officinals and its jiuzhipin by high performance liquid chromatography triple quadrupole mass spectrometry combined with multiple reac

    abstract:OBJECTIVE:The present study examined the pharmacokinetic profiles of two iridoid glycosides named morroniside and loganin in rat plasma after oral administration of crude and processed Cornus officinals. MATERIALS AND METHODS:A rapid, selective and specific high-performance liquid chromatography/electrospray ionizatio...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.127358

    authors: Chen X,Cao G,Jiang J

    更新日期:2014-01-01 00:00:00