Abstract:
OBJECTIVE:To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. MATERIALS AND METHODS:Derivatization with reconstituted AQC was used to prepare the samples and standards for injection into the chromatography column. The peaks were analyzed by fluorescence detection (λ excitation, 250 nm; λ emission, 395 nm. RESULTS:Alanine, proline, and leucine were the most abundant amino acids, whereas tyrosine and methionine were the least abundant. The linearity of the method was found to be good with amino acid concentrations of 0.012-0.36 μM. The precision was 0.05-1.36%; average recovery, 91.12-129.41%; and limit of detection, 0.006-0.058 μM. CONCLUSION:The method is reliable for determining the free amino acid content of different types of chamomile flowers.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Ma X,Zhao D,Li X,Meng Ldoi
10.4103/0973-1296.149735subject
Has Abstractpub_date
2015-01-01 00:00:00pages
176-9issue
41eissn
0973-1296issn
0976-4062pii
PM-11-176journal_volume
11pub_type
杂志文章abstract:BACKGROUND:Rhizoma dioscoreae nipponicae is a perennial herb and its roots have been widely used in Traditional Chinese Medicine (TCM). OBJECTIVE:To develop and optimize the extraction and hydrolysis technology of diosgenin from Rhizoma dioscoreae nipponicae. MATERIALS AND METHODS:1-methyl-3-(3-sulfopropyl)-imidazoli...
journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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