Chromatographic method for determination of the free amino acid content of chamomile flowers.

Abstract:

OBJECTIVE:To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. MATERIALS AND METHODS:Derivatization with reconstituted AQC was used to prepare the samples and standards for injection into the chromatography column. The peaks were analyzed by fluorescence detection (λ excitation, 250 nm; λ emission, 395 nm. RESULTS:Alanine, proline, and leucine were the most abundant amino acids, whereas tyrosine and methionine were the least abundant. The linearity of the method was found to be good with amino acid concentrations of 0.012-0.36 μM. The precision was 0.05-1.36%; average recovery, 91.12-129.41%; and limit of detection, 0.006-0.058 μM. CONCLUSION:The method is reliable for determining the free amino acid content of different types of chamomile flowers.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Ma X,Zhao D,Li X,Meng L

doi

10.4103/0973-1296.149735

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

176-9

issue

41

eissn

0973-1296

issn

0976-4062

pii

PM-11-176

journal_volume

11

pub_type

杂志文章
  • New ionic liquid-based preparative method for diosgenin from Rhizoma dioscoreae nipponicae.

    abstract:BACKGROUND:Rhizoma dioscoreae nipponicae is a perennial herb and its roots have been widely used in Traditional Chinese Medicine (TCM). OBJECTIVE:To develop and optimize the extraction and hydrolysis technology of diosgenin from Rhizoma dioscoreae nipponicae. MATERIALS AND METHODS:1-methyl-3-(3-sulfopropyl)-imidazoli...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.113282

    authors: Yan W,Ji L,Hang S,Shun Y

    更新日期:2013-07-01 00:00:00

  • Comparative analgesic effect of Ligusticum chuanxiong pieces and its products in mice.

    abstract::The present study was undertaken with the objective of finding out the comparative analgesic effect of Ligusticum chuanxiong (LC) pieces decoction, LC formula granule decoction, liquored LC pieces decoction and liquored LC formula granule decoction. The analgesic effects were analyzed using the hot plate and acetic-in...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.62902

    authors: Gao D,Xu L

    更新日期:2010-04-01 00:00:00

  • Cytoprotective and antioxidant effects of phenolic compounds from Haberlea rhodopensis Friv. (Gesneriaceae).

    abstract:BACKGROUND:Haberlea rhodopensis Friv. (Gesneriaceae) is a rare poikilohydric endemic and preglacial relict growing in Balkan Peninsula. Previous investigations demonstrated strong antioxidant, antimicrobial and antimutagenic potential of alcoholic extract from the plant. OBJECTIVE:The isolation of known caffeoyl pheny...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.117822

    authors: Kondeva-Burdina M,Zheleva-Dimitrova D,Nedialkov P,Girreser U,Mitcheva M

    更新日期:2013-10-01 00:00:00

  • Chemical composition and resistance-modifying effect of the essential oil of Lantana camara Linn.

    abstract::In this work, the chemical constituents, antibacterial and modulatory activities of the essential oil of Lantana camara Linn were studied. The essential oil was extracted from the leaves of L. camara by hydrodistillation method using Clevenger's apparatus and its chemical constituents were separated and identified by ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.62890

    authors: Sousa EO,Silva NF,Rodrigues FF,Campos AR,Lima SG,Costa JG

    更新日期:2010-04-01 00:00:00

  • Prim-O-glucosylcimifugin Attenuates Lipopolysaccharideinduced Inflammatory Response in RAW 264.7 Macrophages.

    abstract:BACKGROUND:Radix Saposhnikoviae (RS) exerts anti-inflammatory, analgesic, antipyretic, antioxidation effects and has been used in traditional Chinese medicine to treat common colds, headache, and rheumatoid arthritis. Prim-O-glucosylcimifugin (POG) is the highest content chromone and one of the major active constituent...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_323_16

    authors: Zhou J,Sun YY,Sun MY,Mao WA,Wang L,Zhang J,Zhang H

    更新日期:2017-07-01 00:00:00

  • Exploring the Cytotoxic Potential of Triterpenoids-enriched Fraction of Bacopa monnieri by Implementing In vitro, In vivo, and In silico Approaches.

    abstract:Background:Bacopa monnieri (BM) is a herbaceous plant traditionally used from time immemorial in Ayurvedic and folklore medicines. We hypothesized that the extract of the whole plant might contain numerous molecules with having antitumor activities that could be very effective in killing of human cancer cells. Objecti...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_397_16

    authors: Mallick MN,Khan W,Parveen R,Ahmad S,Sadaf,Najm MZ,Ahmad I,Husain SA

    更新日期:2017-10-01 00:00:00

  • Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials.

    abstract::Mentha piperita essential oil was bactericidal in order of E. coli> S. aureus > Pseudomonas aeruginosa > S. faecalis > Klebsiella pneumoniae. The oil with total phenolics of 89.43 ± 0.58 µg GAE/mg had 63.82 ± 0.05% DPPH inhibition activity with an IC (50) = 3.9 µg/ml. Lipid peroxidation inhibition was comparable to BH...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.66926

    authors: Sharafi SM,Rasooli I,Owlia P,Taghizadeh M,Astaneh SD

    更新日期:2010-07-01 00:00:00

  • Comparative Proteomic Analysis of Three Gelatinous Chinese Medicines and Their Authentications by Tryptic-digested Peptides Profiling using Matrix-assisted Laser Desorption/Ionization-time of Flight/Time of Flight Mass Spectrometry.

    abstract:Background:Gelatinous Chinese medicines (GCMs) including Asini Corii Colla, Testudinis Carapacis ET Plastri Colla, and Cervi Cornus Colla, were made from reptile shell or mammalian skin or deer horn, and consumed as a popular tonic, as well as hemopoietic and hemostatic agents. Misuse of them would not exert their func...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_54_17

    authors: Yang H,Zheng J,Wang HY,Li N,Yang YY,Shen YP

    更新日期:2017-10-01 00:00:00

  • Sterols from the red algae, Gracilaria salicornia and Hypnea flagelliformis, from Persian Gulf.

    abstract:CONTEXT:Two of the important algae from Persian Gulf are Gracilaria salicornia and Hypnea flageliformis (Rhodophyta). Antibacterial, antifungal, and cytotoxic effects of the mentioned algae have been presented in the previous studies. AIM:In this study, the isolation and structural elucidation of the sterols from thes...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.80663

    authors: Nasir M,Saeidnia S,Mashinchian-Moradi A,Gohari AR

    更新日期:2011-04-01 00:00:00

  • Anticancer potential of Conium maculatum extract against cancer cells in vitro: Drug-DNA interaction and its ability to induce apoptosis through ROS generation.

    abstract:OBJECTIVE:Conium maculatum extract is used as a traditional medicine for cervix carcinoma including homeopathy. However, no systematic work has so far been carried out to test its anti-cancer potential against cervix cancer cells in vitro. Thus, in this study, we investigated whether ethanolic extract of conium is capa...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.139792

    authors: Mondal J,Panigrahi AK,Khuda-Bukhsh AR

    更新日期:2014-08-01 00:00:00

  • New Synthetic Pyrazine Carboxamide Derivatives as Potential Elicitors in Production of Secondary Metabolite in In vitro Cultures.

    abstract:BACKGROUND:Silymarin, an active polyphenolic fraction of Silybum marianum, and high flavonoid content of Fagopyrum possess various interesting biological activities. The substituted pyrazine-2-carboxamides were previously used as effective elicitors of studied secondary metabolites. OBJECTIVE:To study the effect of ne...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.176117

    authors: Tůmová L,Tůma J,Doležal M,Dučaiová Z,Kubeš J

    更新日期:2016-01-01 00:00:00

  • Optimization Extraction Process of Polysaccharides from Suillus granulatus and Their Antioxidant and Immunological Activities In vitro.

    abstract:BACKGROUND:Suillus granulatus is an edible and medicinal fungus in China. S. granulatus polysaccharide (SGP) was considered as the main bioactivity compounds in S. granulatus. Therefore, the extraction of SGP and their antioxidant activities were studied in this work. MATERIALS AND METHODS:Fruiting bodies of S. granul...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.182161

    authors: Zhou F,Yan S,Chen S,Gong L,Su T,Wang Z

    更新日期:2016-05-01 00:00:00

  • Secondary Metabolites from Leaves of Manilkara subsericea (Mart.) Dubard.

    abstract:BACKGROUND:Manilkara subsericea (Sapotaceae) is a species widely spread in the sandbanks of Restinga de Jurubatiba National Park (Rio de Janeiro, Brazil). It is commonly known as "maçaranduba", "maçarandubinha" and "guracica", being used in this locality as food, and timber. However, M. subsericea remains almost unexpl...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.172957

    authors: de Almeida FB,Fernandes CP,Romao W,Vanini G,Costa HB,França HS,Santos MG,Carvalho JC,Falcão DQ,Rocha L

    更新日期:2015-10-01 00:00:00

  • Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in A549 human lung cancer cells.

    abstract:BACKGROUND:Methyl protodioscin (MPD) is a furostanol bisglycoside with antitumor properties. It has been shown to reduce proliferation, cause cell cycle arrest. OBJECTIVE:The present study elucidates the mechanism underlying MPD's apoptotic effects, using the A549 human lung cancer cell line. MATERIALS AND METHODS:Th...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.137373

    authors: Bai Y,Qu XY,Yin JQ,Wu L,Jiang H,Long HW,Jia Q

    更新日期:2014-07-01 00:00:00

  • Repellant and insecticidal activities of shyobunone and isoshyobunone derived from the essential oil of Acorus calamus rhizomes.

    abstract:CONTEXT:It was found that the essential oil of Acorus calamus rhizomes showed insecticidal activity. AIM:The aim of this study was to determine the chemical composition of the essential oil from A. calamus rhizomes, evaluate insecticidal and repellant activity against Lasioderma serricorne (LS) and Tribolium castaneum...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.165543

    authors: Chen HP,Yang K,Zheng LS,You CX,Cai Q,Wang CF

    更新日期:2015-10-01 00:00:00

  • Toxicological and biochemical studies on Schinus terebinthifolius concerning its curative and hepatoprotective effects against carbon tetrachloride-induced liver injury.

    abstract:BACKGROUND:Recently, many efforts have been made to discover new products of natural origin which can limit the xenobiotic-induced hepatic injury. Carbon tetrachloride (CCl4) is a highly toxic chemical that is widely used to study hepatotoxicity in animal models. OBJECTIVE:The present study was conducted to investigat...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.157705

    authors: Abdou RH,Saleh SY,Khalil WF

    更新日期:2015-05-01 00:00:00

  • Methanolic Extract of Costus pictus D. DON Induces Cytotoxicity in Liver Hepatocellular Carcinoma Cells Mediated by Histone Deacetylase Inhibition.

    abstract:Background:Leaves of Costus pictus D. Don, (insulin plant) are used as dietary supplement for the treatment of diabetes. Objective:The antidiabetic activity of this plant is well documented, but its activity on different cell types and mechanism remains unknown. Thus, the present study evaluates the cytotoxicity of C....

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_524_16

    authors: Neethu PV,Suthindhiran K,Jayasri MA

    更新日期:2017-10-01 00:00:00

  • Antimicrobial activity of Bauhinia tomentosa and Bauhinia vahlii roots.

    abstract::The hexane, ethylacetate and methanol extracts from Bauhinia tomentosa and Bauhinia vahlii roots were tested for their antimicrobial activity against Gram-positive bacteria (four strains), Gram-negative bacteria (three strains) and three fungi strains using microdilution methods, for the determination of minimal inhib...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.66937

    authors: Dugasani S,Balijepalli MK,Tandra S,Pichika MR

    更新日期:2010-07-01 00:00:00

  • Effect of Shankhpushpi on Alcohol Addiction in Mice.

    abstract::Alcohol addiction is a worldwide problem. It has mainly two components: dependence and withdrawal. Characteristic properties of most anti-addictive compounds include anti-anxiety, anticonvulsant, antidepressant, and nootropic actions. Shankhpushpi (Convolvulus pluricaulis. Convolvulaceae), known ethnopharmacologically...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.203976

    authors: Heba M,Faraz S,Banerjee S

    更新日期:2017-01-01 00:00:00

  • Rapid Screening and Characterization of Acetylcholinesterase Inhibitors from Yinhuang Oral Liquid Using Ultrafiltration-liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry.

    abstract:Background:At present, approximately 17-25 million people in the world suffer from Alzheimer's disease (AD). The most efficacious and acceptable therapeutic drug clinically are the acetylcholinesterase inhibitors (AChEIs). Yinhuang oral liquid is a Chinese medicine preparation which contains AChEIs according to the lit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_166_17

    authors: Zhang H,Guo Y,Meng L,Sun H,Yang Y,Gao Y,Sun J

    更新日期:2018-04-01 00:00:00

  • Capsaicin reverses the inhibitory effect of licochalcone A/β-Arbutin on tyrosinase expression in b16 mouse melanoma cells.

    abstract:Introduction:Melanin is synthesized by melanocytes, which are located in the basal layer of the skin. After synthesis, melanin is further deposited on the surface of the skin to form black spots or chloasma. Tyrosinase is a rate-limiting enzyme that plays an important role in melanogenesis. Currently, there are many dr...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_103_17

    authors: Hong JH,Chen HJ,Xiang SJ,Cao SW,An BC,Ruan SF,Zhang B,Weng LD,Zhu HX,Liu Q

    更新日期:2018-01-01 00:00:00

  • Comparison of pharmacokinetic behavior of two iridoid glycosides in rat plasma after oral administration of crude Cornus officinals and its jiuzhipin by high performance liquid chromatography triple quadrupole mass spectrometry combined with multiple reac

    abstract:OBJECTIVE:The present study examined the pharmacokinetic profiles of two iridoid glycosides named morroniside and loganin in rat plasma after oral administration of crude and processed Cornus officinals. MATERIALS AND METHODS:A rapid, selective and specific high-performance liquid chromatography/electrospray ionizatio...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.127358

    authors: Chen X,Cao G,Jiang J

    更新日期:2014-01-01 00:00:00

  • Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium.

    abstract:BACKGROUND:Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. MATERIALS AND METHODS:The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied th...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.113260

    authors: Guo CY,Wang J,Hou Y,Zhao YM,Shen LX,Zhang DS

    更新日期:2013-07-01 00:00:00

  • Evaluate the Antigenotoxicity and Anticancer Role of β-Sitosterol by Determining Oxidative DNA Damage and the Expression of Phosphorylated Mitogen-activated Protein Kinases', C-fos, C-jun, and Endothelial Growth Factor Receptor.

    abstract:BACKGROUND:Plant sterols are the major source of micronutrients and have not shown any obvious side effects in human. β-sitosterol is one of the most prevalent phytosterols which have been recorded in ancient medicinal history for its use in the treatment of many chronic diseases, especially cancer. The modulations of ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.197634

    authors: Sharmila R,Sindhu G

    更新日期:2017-01-01 00:00:00

  • Evaluation of antidiabetic, antioxidant and antiglycating activities of the Eysenhardtia polystachya.

    abstract:BACKGROUND:Many diseases are associated with oxidative stress caused by free radicals. The aim of the present study was to evaluate the antidiabetic, antioxidant and antiglycation properties of Eysenhardtia polystachya (EP) bark methanol-water extract. MATERIALS AND METHODS:: The antioxidant capacities were evaluated ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.133295

    authors: Gutierrez RM,Baez EG

    更新日期:2014-04-01 00:00:00

  • Culture Conditions for Production of Biomass, Adenosine, and Cordycepin from Cordyceps sinensis CS1197: Optimization by Desirability Function Method.

    abstract:BACKGROUND:Cordyceps sinensis (CS) is a traditional Chinese medicine contains potent active metabolites such as nucleosides and polysaccharides. The submerged cultivation technique is studied for the large scale production of CS for biomass and metabolites production. OBJECTIVE:To optimize culture conditions for large...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.168946

    authors: Ghatnur SM,Parvatam G,Balaraman M

    更新日期:2015-10-01 00:00:00

  • Microwave-assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity.

    abstract:BACKGROUND:Microwave-assisted reflux extraction of polysaccharides YPF-P from the famous Chinese traditional drug, Yupingfeng powder, optimization of extracting conditions and evaluation of their antioxidant activity were conducted in this study. RESULTS:Single factor effect trends were achieved through yields and con...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.160468

    authors: Wang D,Zhang BB,Qu XX,Gao F,Yuan MY

    更新日期:2015-07-01 00:00:00

  • Neuroprotective activity of the methanolic extract of Lonicera japonica in glutamate-injured primary rat cortical cells.

    abstract:BACKGROUND:We previously reported that the extracts of several Korean medicinal plants showed neuroprotective activity in glutamate-injured primary culutres of rat cortical cells. OBJECTIVE:Among them, the effect of the methanolic extract of Lonicera japonica flower on the glutamate-induced neuronal cell death and its...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.90404

    authors: Weon JB,Yang HJ,Lee B,Yun BR,Ahn JH,Lee HY,Ma CJ

    更新日期:2011-10-01 00:00:00

  • A new flavan-3-ol dimer from Ficus spragueana leaves and its cytotoxic activity.

    abstract:BACKGROUND:Isolation and structure elucidation of flavan-3-ol constituents from the leaves of Ficus spragueana and their cytotoxic activity. MATERIALS AND METHODS:Different open silica gel column chromatographic techniques with different solvent systems were used for the separation of the constituents of the ethyl ace...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.111274

    authors: Ragab EA,Mohammed Ael-S,Abbass HS,Kotb SI

    更新日期:2013-04-01 00:00:00

  • Pyrostegia venusta (Ker Gawl.) Miers Crude Extract and Fractions: Prevention of Dental Biofilm Formation and Immunomodulatory Capacity.

    abstract:BACKGROUND:Caries and periodontal diseases remain as important diseases in the Brazilian population. One important pathogen associated with this situation is Streptococcus mutans and other important factor is this pathogen's ability to adhere firmly to the tooth surface leading to dental biofilm formation and caries de...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.182150

    authors: de Sousa MB,Júnior JO,Barbosa WL,da Silva Valério E,da Mata Lima A,de Araújo MH,Muzitano MF,Nakamura CV,de Mello JC,Teixeira FM

    更新日期:2016-05-01 00:00:00