Preparation of Sesquiterpenoids from Tussilago farfara L. by High-speed Counter-current Chromatography.

Abstract:

BACKGROUND:Sesquiterpenoids, such as tussilagone, has effects of raising blood pressure, antiplatelet aggregation, and anti-inflammation activities, which is regarded as index compound for quality control of Tussilago farfara L. OBJECTIVE:This study was aimed to obtain an effective method for fast isolation of sesquiterpenoids from T. farfara L. by high-speed counter-current chromatography (HSCCC). MATERIALS AND METHODS:A solvent optimization method for HSCCC was presented, i.e., the separation factors of compounds after the K values of solvent system should be investigated. RESULTS:A ternary solvent system of n-hexane:methanol:water (5:8:2, v/v/v) was selected and applied for the HSCCC, and 56 mg of tussilagone (2) was isolated from T. farfara L., along with two other sesquiterpenoids 5.6 mg of 2,2-dimethyl-6-acetylchromanone (1) and 22 mg of 14-acetoxy-7 β-(3'-ethyl cis-crotonoyloxy)-lα-(2'-methylbutyryloxy)-notonipetranone (3) by HSCCC with high purities. Their chemical structures were elucidated by liquid chromatography-mass spectrometry and nuclear magnetic resonance experiments. CONCLUSION:These results offered an efficient strategy for preparation of potentially health-relevant phytochemicals from T. farfara L., which might be used for further chemical research and pharmacological studies by preparative HSCCC. SUMMARY:The real separation efficiency has been verified by analytical HSCCC.A solvent optimization method for HSCCC was presented and applied to separate and prepare active compounds.A method for rapid and effective separation of target compound Tussilagone with high yield and purity from the flower buds of Tussilago farfara.Two other compounds 2,2-Dimethyl-6-acetylchromanone and 14-acetoxy-7β-(3'-ethyl cis-crotonoyloxy) -lα- (2'-methylbutyryloxy). notonipetranone hasbeen obtained with high purities from flower buds of Tussilago farfara. Abbreviations used: HSCCC: High-Speed Counter-Current Chromatography; LC-MS: Liquid Chromatograph-Mass Spectrometer; NMR: Nuclear Magnetic Resonance; TCM: Traditional Chinese Medicine; HPLC: High Performance Liquid Chromatography; ESI-MS: Electrospray Ionization Mass Spectrometry; PE: petroleum ether.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Cao K,Xu Y,Zhao TM,Zhang Q

doi

10.4103/0973-1296.192196

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

282-287

issue

48

eissn

0973-1296

issn

0976-4062

pii

PM-12-282

journal_volume

12

pub_type

杂志文章
  • New free radical scavenging neolignans from fruits of Piper attenuatum.

    abstract:OBJECTIVE:The aim was to study and identify free radicals scavenging and antihyperglycemic principles in fruit of Piper attenuatum. MATERIALS AND METHODS:Bioassay guided identification of extracts possessing potent free radical scavenging activity, and isolation of compounds was done. Chloroform extract of P. attenuat...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.153063

    authors: Reddy SD,Siva B,Poornima B,Kumar DA,Tiwari AK,Ramesh U,Babu KS

    更新日期:2015-04-01 00:00:00

  • Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

    abstract:BACKGROUND:Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication. OBJECTIVE:To establish and optimize the rapid propagation technology of S. flavesce...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.71793

    authors: Kun-Hua W,Shan-Lin G,He-Ping H

    更新日期:2010-10-01 00:00:00

  • Purified Essential Oil from Ocimum sanctum Linn. Triggers the Apoptotic Mechanism in Human Breast Cancer Cells.

    abstract:BACKGROUND:Essential oil of Ocimum sanctum Linn. exhibited various pharmacological activities including antifungal and antimicrobial activities. In this study, we analyzed the anticancer and apoptosis mechanisms of Ocimum sanctum essential oil (OSEO). OBJECTIVE:To trigger the apoptosis mechanism in human breast cancer...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.185738

    authors: Manaharan T,Thirugnanasampandan R,Jayakumar R,Kanthimathi MS,Ramya G,Ramnath MG

    更新日期:2016-05-01 00:00:00

  • Anti-hepatocarcinogenic and Anti-oxidant Effects of Mangrove Plant Scyphiphora hydrophyllacea.

    abstract:CONTEXT:Scyphiphora hydrophyllacea is a shrub mangrove plant of the family Rubiaceae and not yet been studied for anti-hepatocarcinogenic effects. OBJECTIVES:We investigated possible in vitro anti-hepatocarcinogenic and antioxidant properties of S. hydrophyllacea. MATERIALS AND METHODS:Dried leaves of S. hydrophyllac...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.203989

    authors: Samarakoon SR,Shanmuganathan C,Ediriweera MK,Piyathilaka P,Tennekoon KH,Thabrew I,Galhena P,De Silva ED

    更新日期:2017-01-01 00:00:00

  • Neuroprotective activity of the methanolic extract of Lonicera japonica in glutamate-injured primary rat cortical cells.

    abstract:BACKGROUND:We previously reported that the extracts of several Korean medicinal plants showed neuroprotective activity in glutamate-injured primary culutres of rat cortical cells. OBJECTIVE:Among them, the effect of the methanolic extract of Lonicera japonica flower on the glutamate-induced neuronal cell death and its...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.90404

    authors: Weon JB,Yang HJ,Lee B,Yun BR,Ahn JH,Lee HY,Ma CJ

    更新日期:2011-10-01 00:00:00

  • A Comprehensive Study on Fast Dispersible and Slow-Releasing Characteristic of Orange Peel Pectin in Relation to Established Synthetic Polymer.

    abstract:Introduction:In the present work, the method to extract, isolate, and characterize orange peel pectin using soxhlation, and thereafter, the use of this polymer-polymer in the formulation of fast dispersable and slow-releasing tablet has been studied. Thereafter, the evaluation and comparison of fast dispersible/slow-re...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_193_17

    authors: Srivastava P,Singh M,Bhargava S

    更新日期:2017-10-01 00:00:00

  • 7-hydroxycalamenene Effects on Secreted Aspartic Proteases Activity and Biofilm Formation of Candida spp.

    abstract:BACKGROUND:The 7-hydroxycalamenenene-rich essential oil (EO) obtained from the leaves of Croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. In this work, we aimed to evaluate the effectiveness of 7-hydroxycalamenenene against Candida albicans and nonalbicans sp...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.176022

    authors: Azevedo MM,Almeida CA,Chaves FC,Rodrigues IA,Bizzo HR,Alviano CS,Alviano DS

    更新日期:2016-01-01 00:00:00

  • Methanolic Extract of Costus pictus D. DON Induces Cytotoxicity in Liver Hepatocellular Carcinoma Cells Mediated by Histone Deacetylase Inhibition.

    abstract:Background:Leaves of Costus pictus D. Don, (insulin plant) are used as dietary supplement for the treatment of diabetes. Objective:The antidiabetic activity of this plant is well documented, but its activity on different cell types and mechanism remains unknown. Thus, the present study evaluates the cytotoxicity of C....

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_524_16

    authors: Neethu PV,Suthindhiran K,Jayasri MA

    更新日期:2017-10-01 00:00:00

  • Species identification of Rhododendron (Ericaceae) using the chloroplast deoxyribonucleic acid PsbA-trnH genetic marker.

    abstract:BACKGROUND:Rhododendron is a group of famous landscape plants with high medicinal value. However, there is no simple or universal manner to discriminate the various species of this group. Deoxyribonucleic acid (DNA) barcoding technique is a new biological tool that can accurately and objectively identify species by usi...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.93311

    authors: Liu Y,Zhang L,Liu Z,Luo K,Chen S,Chen K

    更新日期:2012-01-01 00:00:00

  • Total Phenolics, Total Flavonoids, Antioxidant Capacities, and Volatile Compounds Gas Chromatography-Mass Spectrometry Profiling of Moringa oleifera Ripe Seed Polar Fractions.

    abstract:Background:Academic reports have confirmed Moringa oleifera leaves to possess significant antioxidant capacities; however, such studies are unavailable for its ripe seeds even though they are more desirous for consumption due to their sweet taste. Objective:In this study, we investigated antioxidant capacities of four...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_212_17

    authors: Adebayo IA,Arsad H,Samian MR

    更新日期:2018-04-01 00:00:00

  • Optimization of Ultrasound-assisted extraction of polyphenols, tannins and epigallocatechin gallate from barks of Stryphnodendron adstringens (Mart.) Coville bark extracts.

    abstract:BACKGROUND:Stryphnodendron adstringens (Mar.) Coville is a native plant from Brazil, rich in phenolic compounds and used on popular medicine as a wound healing agent, in the treatment of gastric lesions and as antimicrobial. MATERIALS AND METHODS:Ultrassound-assisted extraction (UAE) was applied to extraction of epiga...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.133287

    authors: Sousa JN,Pedroso NB,Borges LL,Oliveira GA,Paula JR,Conceição EC

    更新日期:2014-04-01 00:00:00

  • Chemical composition and resistance-modifying effect of the essential oil of Lantana camara Linn.

    abstract::In this work, the chemical constituents, antibacterial and modulatory activities of the essential oil of Lantana camara Linn were studied. The essential oil was extracted from the leaves of L. camara by hydrodistillation method using Clevenger's apparatus and its chemical constituents were separated and identified by ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.62890

    authors: Sousa EO,Silva NF,Rodrigues FF,Campos AR,Lima SG,Costa JG

    更新日期:2010-04-01 00:00:00

  • Antioxidant activities and fatty acid composition of wild grown myrtle (Myrtus communis L.) fruits.

    abstract::The fruits of eight myrtles, Myrtus communis L. accessions from the Mediterranean region of Turkey were evaluated for their antioxidant activities and fatty acid contents. The antioxidant activities of the fruit extracts were determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene-linoleic acid assa...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.59960

    authors: Serce S,Ercisli S,Sengul M,Gunduz K,Orhan E

    更新日期:2010-01-01 00:00:00

  • Phytochemicals and Cytotoxicity of Launaea procumbens on Human Cancer Cell Lines.

    abstract:BACKGROUND:The plant Launaea procumbens belongs to the family Asteraceae and traditionally used in the treatment rheumatism, kidney, liver dysfunctions and eye diseases. In the present study Phytochemical analysis and fractions of methanolic extract of L. procumbens leaves were tested in vitro for their cytotoxicity. ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.191452

    authors: Rawat P,Saroj LM,Kumar A,Singh TD,Tewari SK,Pal M

    更新日期:2016-07-01 00:00:00

  • Liquid Chromatography Mass Spectrometry Analysis and Cytotoxicity of Asparagus adscendens Roots against Human Cancer Cell Lines.

    abstract:Background:Asparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. This plant has been used in the prevention and effective treatment of various forms of cancers. Objective:This paper reports, for the first time, on the cytotoxicity of the methanol (MeOH) extract of the roots of A. adscendens and its so...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_136_17

    authors: Khan KM,Nahar L,Mannan A,Arfan M,Khan GA,Al-Groshi A,Evans A,Dempster NM,Ismail FMD,Sarker SD

    更新日期:2018-01-01 00:00:00

  • Isolation and Characterisation of a Proanthocyanidin With Antioxidative, Antibacterial and Anti-Cancer Properties from Fern Blechnum orientale.

    abstract:BACKGROUND:Blechnum orientale Linn. (Blechnaceae), a fern, is traditionally used in the treatment of various ailments, such as skin diseases, stomach pain, urinary bladder complaints, and also as a female contraceptive. Previously, we reported a strong radical scavenging activity, antibacterial activity and cytotoxicit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.197659

    authors: Lai HY,Lim YY,Kim KH

    更新日期:2017-01-01 00:00:00

  • Pretreatment with Assafoetida exerts dose-dependent dual effects on rat hearts.

    abstract:CONTEXT:Although many studies displayed the favorable effects of Assafoetida, some of them reported that high doses of Assafoetida could lead to harmful effects. AIMS:In this study, the effect of pretreatment with Assafoetida investigated on ischemic-reperfusion injury in isolated rat heart model. MATERIALS AND METHO...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.131026

    authors: Esmailidehaj M,Kakoo M,Rezvani ME,Mosaddeghmehrjardi MH

    更新日期:2014-04-01 00:00:00

  • Chromatographic method for determination of the free amino acid content of chamomile flowers.

    abstract:OBJECTIVE:To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. MATERIALS AND METHODS:Derivatization wit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.149735

    authors: Ma X,Zhao D,Li X,Meng L

    更新日期:2015-01-01 00:00:00

  • Comparative analgesic effect of Ligusticum chuanxiong pieces and its products in mice.

    abstract::The present study was undertaken with the objective of finding out the comparative analgesic effect of Ligusticum chuanxiong (LC) pieces decoction, LC formula granule decoction, liquored LC pieces decoction and liquored LC formula granule decoction. The analgesic effects were analyzed using the hot plate and acetic-in...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.62902

    authors: Gao D,Xu L

    更新日期:2010-04-01 00:00:00

  • In vitro α-amylase and α-glucosidase inhibitory effects and cytotoxic activity of Albizia antunesiana extracts.

    abstract:CONTEXT:Diabetes mellitus is a chronic disease, and its incidence is tremendously increasing globally. Decreasing postprandial hyperglycemia by retarding glucose absorption through inhibiting carbohydrates digesting enzymes (α-amylase and α-glucosidase) is one of many approaches used for the management of this disease....

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.166018

    authors: Chipiti T,Ibrahim MA,Singh M,Islam MS

    更新日期:2015-10-01 00:00:00

  • Antioxidant and anti-lipoxygenase activities of extracts from different parts of Lavatera cretica L. grown in Algarve (Portugal).

    abstract:BACKGROUND:Lavatera cretica L. was used in folk medicine as anti-inflammatory among other applications. As inflammation is many times associated with oxidative processes, the aim of the present work was to evaluate the ability of aqueous extracts obtained from different parts of L. cretica to prevent oxidation or infla...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.149743

    authors: Ben-Nasr S,Aazza S,Mnif W,Miguel Mda G

    更新日期:2015-01-01 00:00:00

  • Evaluation of antidiabetic, antioxidant and antiglycating activities of the Eysenhardtia polystachya.

    abstract:BACKGROUND:Many diseases are associated with oxidative stress caused by free radicals. The aim of the present study was to evaluate the antidiabetic, antioxidant and antiglycation properties of Eysenhardtia polystachya (EP) bark methanol-water extract. MATERIALS AND METHODS:: The antioxidant capacities were evaluated ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.133295

    authors: Gutierrez RM,Baez EG

    更新日期:2014-04-01 00:00:00

  • Optimization of Polysaccharide Ultrasonic Extraction Conditions Using Purple Sweet Potato Tubers Based on Free Radical Scavenging and Glycosylation Inhibitory Bioactivities.

    abstract:BACKGROUND:The purple sweet potato, Ipomoea batatas, belongs to the family Convolvulaceae. It is one of the most widely consumed tubers in Asia and is found in many dishes. Many people with diabetes eat purple sweet potato tubers to help reduce blood glucose in China. OBJECTIVE:To predict the ultrasonic conditions for...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.211044

    authors: Guo H,Kong F,Yan C

    更新日期:2017-07-01 00:00:00

  • In vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea teretifolia Willd extracts on human prostate cancer cell lines.

    abstract:BACKGROUND:The majority of Achillea species are the most important native economic plants of Anatolia. They include highly bioactive compounds, so they have therapeutic applications. OBJECTIVE:In the present study, the aim was to investigate in vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea tereti...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.166060

    authors: Bali EB,Açık L,Elçi P,Sarper M,Avcu F,Vural M

    更新日期:2015-10-01 00:00:00

  • Chemical constituents with free-radical-scavenging activities from the stem of Fissistigma polyanthum.

    abstract:BACKGROUND:Fissistigma polyanthum is a liane belonging to the Annonaceae family and it is one of the most important crude drugs in traditional Chinese medicine. OBJECTIVE:The objective was to describe the structural elucidation and the free-radical-scavenging activities of the isolated compounds from Fissistigma polya...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.96549

    authors: Fan H,Zheng T,Chen Y,Yang GZ

    更新日期:2012-04-01 00:00:00

  • Safety Profile Investigations of Meyna spinosa (Roxb.) and Oroxylum indicum (Linn.) Extracts Collected from Northeast India.

    abstract:Background:Meyna spinosa (M.S) (Roxb.) ex Link and Oroxylum indicum (O.I) (Linn.) Vent, widely used traditional Northeast Indian medicinal plant used for various purposes, have not yet explored for safety profile. Objective:To investigate the safety profile of M.S (Roxb.) ex Link leaves and O.I (Linn.) Vent stem bark ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_62_15

    authors: Singh S,Chattopadhyay P,Borthakur SK,Policegoudra R

    更新日期:2018-01-01 00:00:00

  • Pharmacokinetics, intestinal absorption and microbial metabolism of single platycodin D in comparison to Platycodi radix extract.

    abstract:BACKGROUND:Platycodi radix, the dried root of Platycodon grandiflorum A. DC, has been widely used as food and herb medicine for treating cough, cold and other respiratory ailments, and platycodin D (PD) is one of the most important compounds in Platycodi Radix. OBJECTIVE:The purpose of this study was to compare the ph...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.165576

    authors: Shan J,Zou J,Xie T,Kang A,Zhou W,Deng H,Mao Y,Di L,Wang S

    更新日期:2015-10-01 00:00:00

  • Quantitative determination of total and individual flavonoids in stems and leaves of Buddleja davidii and Buddleja albiflora.

    abstract:BACKGROUND:Buddleja davidii and B. albiflora are two different original plants of the famous crude medicine "Diaoyangchen". MATERIALS AND METHODS:An ultraviolet-visible spectrophotometric method and a HPLC method were used for the determination of total and individual flavonoids (luteolin and apigenin) contents from t...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.103651

    authors: Ying C,Wan D

    更新日期:2012-10-01 00:00:00

  • Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts.

    abstract:BACKGROUND:Hakea sericea Schrader is an invasive shrub in Portuguese forests. OBJECTIVE:The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.127331

    authors: Luís A,Breitenfeld L,Ferreira S,Duarte AP,Domingues F

    更新日期:2014-01-01 00:00:00

  • Identification of Hepatoprotective Constituents in Limonium tetragonum and Development of Simultaneous Analysis Method using High-performance Liquid Chromatography.

    abstract:Background:Limonium tetragonum, a naturally salt-tolerant halophyte, has been studied recently and is of much interest to researchers due to its potent antioxidant and hepatoprotective activities. Objective:In the present study, we attempted to elucidate bioactive compounds from ethyl acetate (EtOAc) soluble fraction ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_477_16

    authors: Lee JS,Kim YN,Kim NH,Heo JD,Yang MH,Rho JR,Jeong EJ

    更新日期:2017-10-01 00:00:00