Abstract:
CONTEXT:Scyphiphora hydrophyllacea is a shrub mangrove plant of the family Rubiaceae and not yet been studied for anti-hepatocarcinogenic effects. OBJECTIVES:We investigated possible in vitro anti-hepatocarcinogenic and antioxidant properties of S. hydrophyllacea. MATERIALS AND METHODS:Dried leaves of S. hydrophyllacea were sequentially extracted into hexane, chloroform, ethyl acetate, and methanol and tested for cytotoxicity on HepG2 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and sulforhodamine B assays, and for antioxidant activities by the free radical 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) assays. Total phenolic and flavonoid contents were estimated in all four extracts. The hexane and chloroform extracts were tested for pro-apoptotic properties in HepG2 cells, and bioactive components were identified by gas chromatography-mass spectrometry (GC-MS) analysis. RESULTS:The hexane and chloroform extracts showed dose-dependent and time-dependent cytotoxic effects. Morphological changes observed under fluorescence microscope related to apoptosis, and significant (P < 0.001) increases in caspase 3 and 9 levels were observed in hexane and chloroform extract-treated cells. Slight DNA fragmentation was observed only in response to the chloroform extract. mRNA expressions of p53 and Bax were significantly upregulated by low doses of hexane and chloroform extracts. Highest antioxidant activity was observed in the methanol extract. GC-MS profiles identified 24 and four major compounds in the hexane and chloroform extracts, respectively. These included some known anticancer compounds such as lupeol. CONCLUSION:Cytotoxicity, antioxidant effects, and apoptosis-related changes exerted by hexane and chloroform extracts of S. hydrophyllacea concluded that these two extracts are good source for isolation of possible anticarcinogenic compounds. SUMMARY:The hexane and chloroform extracts of Scyphiphora hydrophyllacea showed dose-dependent and time-dependent cytotoxic effects.Morphological changes related to apoptosis and significant (P < 0.001) increases in caspase 3 and 9 levels were observed in hexane and chloroform extract-treated cells.mRNA expressions of p53 and Bax were significantly upregulated by low doses of hexane and chloroform extracts.Highest antioxidant activity was observed in the methanol extract.GC-MS profiles identified 24 and four major compounds in the hexane and chloroform extracts, respectively. Abbreviation used: DPPH: 1,1-diphenyl-2-picryl-hydrazyl, ABTS: 2,2'-azinobis-3-ethylbenzthiazoline-6-sulfonic acid, GC-MS: gas chromatography-mass spectrometry, DNA: deoxyribonucleic acid, HCC: Hepatocellular carcinoma, GAE: gallic acid equivalents, SRB: sulforhodamine B, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, AO/EB: acridine orange/ethidium bromide, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, IC50: half maximal inhibitory concentration; QE: quercetin equivalents, HE: hexane extract, CE: chloroform extract, EAE: ethyl acetate extract, ME: methanolic extract, TPC: total polyphenol content, TFC: total flavonoid content, ANOVA: Analysis of variance.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Samarakoon SR,Shanmuganathan C,Ediriweera MK,Piyathilaka P,Tennekoon KH,Thabrew I,Galhena P,De Silva EDdoi
10.4103/0973-1296.203989subject
Has Abstractpub_date
2017-01-01 00:00:00pages
S76-S83issue
Suppl 1eissn
0973-1296issn
0976-4062pii
PM-13-76journal_volume
13pub_type
杂志文章abstract:OBJECTIVE:Conium maculatum extract is used as a traditional medicine for cervix carcinoma including homeopathy. However, no systematic work has so far been carried out to test its anti-cancer potential against cervix cancer cells in vitro. Thus, in this study, we investigated whether ethanolic extract of conium is capa...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.139792
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Piper trichostachyon is a wild, endemic Piper species from Western Ghats of India. The folklore healers of Belagavi region use this plant, similar to Piper nigrum. AIMS:The present study investigates the comparison between P. nigrum and P. trichostachyon using pharmacognostic parameters. MATERIALS AND METH...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.182172
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Lavatera cretica L. was used in folk medicine as anti-inflammatory among other applications. As inflammation is many times associated with oxidative processes, the aim of the present work was to evaluate the ability of aqueous extracts obtained from different parts of L. cretica to prevent oxidation or infla...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.149743
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Rhizoma dioscoreae nipponicae is a perennial herb and its roots have been widely used in Traditional Chinese Medicine (TCM). OBJECTIVE:To develop and optimize the extraction and hydrolysis technology of diosgenin from Rhizoma dioscoreae nipponicae. MATERIALS AND METHODS:1-methyl-3-(3-sulfopropyl)-imidazoli...
journal_title:Pharmacognosy magazine
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更新日期:2013-07-01 00:00:00
abstract:OBJECTIVE:To optimization of extraction of antioxidant compounds from guava (Psidium guajava L.) leaves and showed that the guava leaves are the potential source of antioxidant compounds. MATERIALS AND METHODS:The bioactive polysaccharide compounds of guava leaves (P. guajava L.) were obtained using ultrasonic-assiste...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.160451
更新日期:2015-07-01 00:00:00
abstract:Background:At present, approximately 17-25 million people in the world suffer from Alzheimer's disease (AD). The most efficacious and acceptable therapeutic drug clinically are the acetylcholinesterase inhibitors (AChEIs). Yinhuang oral liquid is a Chinese medicine preparation which contains AChEIs according to the lit...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_166_17
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:The active ingredients and thus pharmacological efficacy of traditional Chinese medicine (TCM) at different degrees of parching process vary greatly. OBJECTIVE:Near-infrared spectroscopy (NIR) was used to develop a new method for rapid online analysis of TCM parching process, using two kinds of chemical ind...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_416_16
更新日期:2017-04-01 00:00:00
abstract:Background:Asparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. This plant has been used in the prevention and effective treatment of various forms of cancers. Objective:This paper reports, for the first time, on the cytotoxicity of the methanol (MeOH) extract of the roots of A. adscendens and its so...
journal_title:Pharmacognosy magazine
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更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The 7-hydroxycalamenenene-rich essential oil (EO) obtained from the leaves of Croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. In this work, we aimed to evaluate the effectiveness of 7-hydroxycalamenenene against Candida albicans and nonalbicans sp...
journal_title:Pharmacognosy magazine
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更新日期:2016-01-01 00:00:00
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更新日期:2013-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Fissistigma polyanthum is a liane belonging to the Annonaceae family and it is one of the most important crude drugs in traditional Chinese medicine. OBJECTIVE:The objective was to describe the structural elucidation and the free-radical-scavenging activities of the isolated compounds from Fissistigma polya...
journal_title:Pharmacognosy magazine
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更新日期:2012-04-01 00:00:00
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journal_title:Pharmacognosy magazine
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abstract:BACKGROUND:Cordyceps sinensis (CS) is a traditional Chinese medicine contains potent active metabolites such as nucleosides and polysaccharides. The submerged cultivation technique is studied for the large scale production of CS for biomass and metabolites production. OBJECTIVE:To optimize culture conditions for large...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.168946
更新日期:2015-10-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.197634
更新日期:2017-01-01 00:00:00
abstract:CONTEXT:Although many studies displayed the favorable effects of Assafoetida, some of them reported that high doses of Assafoetida could lead to harmful effects. AIMS:In this study, the effect of pretreatment with Assafoetida investigated on ischemic-reperfusion injury in isolated rat heart model. MATERIALS AND METHO...
journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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更新日期:2013-10-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2017-01-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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abstract:BACKGROUND:Annona vepretorum (AV) is a native tree from Caatinga biome (semiarid region of Brazil) popularly known as "araticum" and "pinha da Caatinga." OBJECTIVE:This study was carried out to evaluate the chemical constituents and antioxidant activity (AA) of the essential oil from the leaves from AV (EO-Av) collect...
journal_title:Pharmacognosy magazine
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更新日期:2015-07-01 00:00:00
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更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Stryphnodendron adstringens (Mar.) Coville is a native plant from Brazil, rich in phenolic compounds and used on popular medicine as a wound healing agent, in the treatment of gastric lesions and as antimicrobial. MATERIALS AND METHODS:Ultrassound-assisted extraction (UAE) was applied to extraction of epiga...
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更新日期:2014-04-01 00:00:00
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abstract:BACKGROUND:The real mechanism for Thevetia peruviana poisoning remains unclear. Cholinergic activity is important for cardiac function regulation, however, the effect of T. peruviana on cholinergic activity is not well-known. OBJECTIVE:To study the effect of the acute administration of an aqueous extract of the seed k...
journal_title:Pharmacognosy magazine
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更新日期:2014-01-01 00:00:00
abstract::The hexane, ethylacetate and methanol extracts from Bauhinia tomentosa and Bauhinia vahlii roots were tested for their antimicrobial activity against Gram-positive bacteria (four strains), Gram-negative bacteria (three strains) and three fungi strains using microdilution methods, for the determination of minimal inhib...
journal_title:Pharmacognosy magazine
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更新日期:2010-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2010-10-01 00:00:00
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更新日期:2016-07-01 00:00:00