Abstract:
BACKGROUND:Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication. OBJECTIVE:To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens. MATERIALS AND METHODS:The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation. RESULTS:A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4-1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4× = 36. All tetraploid plants showed typical polyploid characteristics. CONCLUSION:Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Kun-Hua W,Shan-Lin G,He-Ping Hdoi
10.4103/0973-1296.71793subject
Has Abstractpub_date
2010-10-01 00:00:00pages
286-92issue
24eissn
0973-1296issn
0976-4062journal_volume
6pub_type
杂志文章abstract:BACKGROUND:Platycodi radix, the dried root of Platycodon grandiflorum A. DC, has been widely used as food and herb medicine for treating cough, cold and other respiratory ailments, and platycodin D (PD) is one of the most important compounds in Platycodi Radix. OBJECTIVE:The purpose of this study was to compare the ph...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.165576
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Tithonia diversifolia belongs to the Asteraceae family. The leaves of T. diversifolia have been studied lately because of the presence of tagitinin C. OBJECTIVE:Looking for an easy and inexpensive method to extract tagitinin C from T. diversifolia leaves, this work aims to conduct a screening to evaluate th...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.204555
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abstract:BACKGROUND:The present study reports and compares the results of Gas Chromatographic-Mass analyses of Pulicaria jaubertii leaf (P-1) and root (P-2) essential oils, as well as their in vitro antimicrobial and cytotoxic activities. MATERIALS AND METHODS:The chemical composition of P-1 and P-2 essential oils of P. jauber...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.108133
更新日期:2013-01-01 00:00:00
abstract::Alpinia purpurata or red ginger was studied for its phytochemical constituents as part of our growing interest on Philippine Zingiberaceae plants that may exhibit antimycobacterial activity. The hexane and dichloromethane subextracts of the leaves were fractionated and purified using silica gel chromatography to affor...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.71785
更新日期:2010-10-01 00:00:00
abstract::Chalcones have been shown to exhibit anti-cancer properties by targeting multiple molecular pathways. It was, therefore, of interest to investigate flavokawain C (FKC), a naturally occurring chalcone, which can be isolated from Kava (Piper methysticum Forst) root extract. The aim of this study was to investigate the i...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.210180
更新日期:2017-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_193_17
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_159_16
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:To optimization of extraction of antioxidant compounds from guava (Psidium guajava L.) leaves and showed that the guava leaves are the potential source of antioxidant compounds. MATERIALS AND METHODS:The bioactive polysaccharide compounds of guava leaves (P. guajava L.) were obtained using ultrasonic-assiste...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.160451
更新日期:2015-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.62902
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:Aronia melanocarpa (Michx.) Elliot fruits are very rich in polyphenols - procyanidins, flavonoids, and phenolic acids. OBJECTIVE:On rat hepatocytes, isolated by two-stepped collagenase perfusion, we investigated the effect of A. melanocarpa fruit juice (AMFJ) in two models of liver toxicity caused by (i) me...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.172967
更新日期:2015-10-01 00:00:00
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journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.168945
更新日期:2015-10-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_489_16
更新日期:2017-10-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_416_16
更新日期:2017-04-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.62890
更新日期:2010-04-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.153063
更新日期:2015-04-01 00:00:00
abstract::Mentha piperita essential oil was bactericidal in order of E. coli> S. aureus > Pseudomonas aeruginosa > S. faecalis > Klebsiella pneumoniae. The oil with total phenolics of 89.43 ± 0.58 µg GAE/mg had 63.82 ± 0.05% DPPH inhibition activity with an IC (50) = 3.9 µg/ml. Lipid peroxidation inhibition was comparable to BH...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.66926
更新日期:2010-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.139792
更新日期:2014-08-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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更新日期:2014-01-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.153094
更新日期:2015-04-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.211044
更新日期:2017-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.182150
更新日期:2016-05-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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更新日期:2018-01-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:The plant Launaea procumbens belongs to the family Asteraceae and traditionally used in the treatment rheumatism, kidney, liver dysfunctions and eye diseases. In the present study Phytochemical analysis and fractions of methanolic extract of L. procumbens leaves were tested in vitro for their cytotoxicity. ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.191452
更新日期:2016-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2015-05-01 00:00:00