Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

Abstract:

BACKGROUND:Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication. OBJECTIVE:To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens. MATERIALS AND METHODS:The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation. RESULTS:A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4-1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4× = 36. All tetraploid plants showed typical polyploid characteristics. CONCLUSION:Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Kun-Hua W,Shan-Lin G,He-Ping H

doi

10.4103/0973-1296.71793

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

286-92

issue

24

eissn

0973-1296

issn

0976-4062

journal_volume

6

pub_type

杂志文章