Abstract:
Introduction:Melanin is synthesized by melanocytes, which are located in the basal layer of the skin. After synthesis, melanin is further deposited on the surface of the skin to form black spots or chloasma. Tyrosinase is a rate-limiting enzyme that plays an important role in melanogenesis. Currently, there are many drugs that inhibit tyrosinase expression to further reduce melanogenesis. Nevertheless, some of these could reverse the pharmacological effect of other drugs, when used simultaneously. Materials and Methods:B16 mouse melanoma cells were treated with the tyrosinase inhibitors licochalcone A and β-arbutin, alone or in combination with capsaicin, an alkaloid found in peppers. Cytotoxicity, melanin content, and tyrosinase activity and expression were determined. Results:Licochalcone A/β-arbutin inhibited tyrosinase expression and further hindered melanin synthesis when applied individually to B16 mouse melanoma cells. However, licochalcone A/β-arbutin combined with 50 μmol/L capsaicin enhanced the expression of tyrosinase in these cells and further increased melanin content. Conclusion:Our data implied that capsaicin could reverse the inhibitory effect of licochalcone A/β-arbutin on tyrosinase expression in B16 mouse melanoma cells. SUMMARY:B16 mouse melanoma cells were treated with the tyrosinase inhibitors licochalcone A and β-arbutin, alone or in combination with capsaicin, an alkaloid found in peppers. Cytotoxicity, melanin content, and tyrosinase activity and expression were determined. Licochalcone A/β-arbutin inhibited tyrosinase expression and further hindered melanin synthesis when applied individually to B16 mouse melanoma cells. However, licochalcone A/β-arbutin combined with 50 μmol/L capsaicin enhanced the expression of tyrosinase in these cells and further increased melanin content. Our research implied that capsaicin could reverse the inhibitory effect of licochalcone A/β-arbutin on tyrosinase expression in B16 mouse melanoma cells. Abbreviations used: B16: B16 mouse melanoma cells; L-DOPA: 3, 4-L-dihydroxyphenylalanine; TYR: Tyrosinase; USP: United States Pharmacopeia; FBS: Fetal bovine serum; EDTA: Ethylenediaminetetraacetic acid; DMSO: Dimethyl sulfoxide; RPMI: Roswell Park Memorial Institute; MTT3: 4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, NaOH: Sodium hydroxide; PBS: Phosphate-buffered saline; RIPA: Radio-immunoprecipitation assay; PMSF: Phenylmethanesulfonyl fluoride or phenylmethylsulfonyl fluoride; SDS: Sodium dodecyl sulfate, sodium salt; PVDF: Polyvinylidene fluoride; ECL: Enhanced chemiluminescence.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Hong JH,Chen HJ,Xiang SJ,Cao SW,An BC,Ruan SF,Zhang B,Weng LD,Zhu HX,Liu Qdoi
10.4103/pm.pm_103_17subject
Has Abstractpub_date
2018-01-01 00:00:00pages
110-115issue
53eissn
0973-1296issn
0976-4062pii
PM-14-110journal_volume
14pub_type
杂志文章abstract:OBJECTIVE:To optimization of extraction of antioxidant compounds from guava (Psidium guajava L.) leaves and showed that the guava leaves are the potential source of antioxidant compounds. MATERIALS AND METHODS:The bioactive polysaccharide compounds of guava leaves (P. guajava L.) were obtained using ultrasonic-assiste...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.160451
更新日期:2015-07-01 00:00:00
abstract:Background:Hypertension is a great global health challenge and it mostly requires drug combination therapy with the various advantages. Metoprolol (MP) and paeoniflorin are both commonly used for the treatment of hypertension. However, whether they exert synergistic effects on antihypertension or not remains unclear, e...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_483_16
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:We previously reported that the extracts of several Korean medicinal plants showed neuroprotective activity in glutamate-injured primary culutres of rat cortical cells. OBJECTIVE:Among them, the effect of the methanolic extract of Lonicera japonica flower on the glutamate-induced neuronal cell death and its...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.90404
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Vatica diospyroides type LS is a known source of valuable compounds for cancer treatment, however, in contrast little is known about therapeutic efficacy of type SS. OBJECTIVE:This study focused on in vitro cytotoxicity of these fruit extracts, and the cell death mode they induce in breast cancer cells. MA...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.157718
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:The present study reports and compares the results of Gas Chromatographic-Mass analyses of Pulicaria jaubertii leaf (P-1) and root (P-2) essential oils, as well as their in vitro antimicrobial and cytotoxic activities. MATERIALS AND METHODS:The chemical composition of P-1 and P-2 essential oils of P. jauber...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.108133
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Zataria multiflora Boiss. is a traditional and popular spice in Iran. The effects of 3 ecotypes (ECTPs) of Z. multiflora essential oils (EOs) against most common causes of food-borne and nosocomial infections were evaluated. MATERIALS AND METHODS:The antimicrobial activities of the EOs were examined by brot...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.75902
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Coffea canephora is the commonly cultivated coffee species in the world along with Coffea arabica. Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particul...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_518_16
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Methyl protodioscin (MPD) is a furostanol bisglycoside with antitumor properties. It has been shown to reduce proliferation, cause cell cycle arrest. OBJECTIVE:The present study elucidates the mechanism underlying MPD's apoptotic effects, using the A549 human lung cancer cell line. MATERIALS AND METHODS:Th...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.137373
更新日期:2014-07-01 00:00:00
abstract:Background:Academic reports have confirmed Moringa oleifera leaves to possess significant antioxidant capacities; however, such studies are unavailable for its ripe seeds even though they are more desirous for consumption due to their sweet taste. Objective:In this study, we investigated antioxidant capacities of four...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_212_17
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:The purple sweet potato, Ipomoea batatas, belongs to the family Convolvulaceae. It is one of the most widely consumed tubers in Asia and is found in many dishes. Many people with diabetes eat purple sweet potato tubers to help reduce blood glucose in China. OBJECTIVE:To predict the ultrasonic conditions for...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.211044
更新日期:2017-07-01 00:00:00
abstract:Background:Moringa oleifera (MO), commonly known as the drumstick tree, is used in folklore medicine for the treatment of skin disease. Objective:The objective of this study is to evaluate the ethyl acetate (EtOAc) fraction of MO leaves for in vitro antibacterial, antioxidant, and wound healing activities and conduct ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_368_16
更新日期:2017-10-01 00:00:00
abstract:AIMS:The study is conducted to evaluate the immunomodulatory, cytotoxicity, and antioxidant potential of Ziziphus mauritiana (Rhamnaceae). Phytochemical analysis of Z. mauritiana revealed the presence of alkaloids, anthraquinone glycoside, cardiac glycoside, saponin, tannin, and flavonoids. METHODOLOGY:The cytotoxicit...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_398_16
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Polygonum multiflorum is an important medicinal plant. Hairy roots systems obtained by transforming plant tissues with the natural genetic engineer Agrobacterium rhizogenes can produce valuable biological active substances, which have immense potential in the pharmaceutical industry. OBJECTIVE:To optimize t...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.126671
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Zingiber officinale Rosc. is not only an important medical plant in China, but also one of the most commonly used plant spices around the world. Early researches in Z. officinale Rosc. were focused on rapid propagation, germplasm preservation, and somatic embryogenesis, only a few reports focused on the gene...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.84230
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:Traditionally, a number of medicinal plants are used to treat various types of hepatic disorders but few of them were pharmacologically evaluated for their safety and efficacy. The combination of Andrographis paniculata (Kalmegha), Tinospora cordifolia (Guduchi), and Solanum nigrum (Kakmachi) was traditional...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.168945
更新日期:2015-10-01 00:00:00
abstract:CONTEXT:Although many studies displayed the favorable effects of Assafoetida, some of them reported that high doses of Assafoetida could lead to harmful effects. AIMS:In this study, the effect of pretreatment with Assafoetida investigated on ischemic-reperfusion injury in isolated rat heart model. MATERIALS AND METHO...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.131026
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:The majority of Achillea species are the most important native economic plants of Anatolia. They include highly bioactive compounds, so they have therapeutic applications. OBJECTIVE:In the present study, the aim was to investigate in vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea tereti...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.166060
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Yinqiaosan (Lonicerae and Forsythiae Powder), as a famous prescription of Dr. Wu Jutong in Qing dynasty of China, has the effects of diaphoresis cooling, fire-purging, and detoxicaton. It is mainly used in the treatment of influenza, hand-foot-mouth disease, esophagitis, pneumonia, acute tonsillitis, mumps, ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.177911
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:Suillus granulatus is an edible and medicinal fungus in China. S. granulatus polysaccharide (SGP) was considered as the main bioactivity compounds in S. granulatus. Therefore, the extraction of SGP and their antioxidant activities were studied in this work. MATERIALS AND METHODS:Fruiting bodies of S. granul...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.182161
更新日期:2016-05-01 00:00:00
abstract:Background:Gelatinous Chinese medicines (GCMs) including Asini Corii Colla, Testudinis Carapacis ET Plastri Colla, and Cervi Cornus Colla, were made from reptile shell or mammalian skin or deer horn, and consumed as a popular tonic, as well as hemopoietic and hemostatic agents. Misuse of them would not exert their func...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_54_17
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Silymarin, an active polyphenolic fraction of Silybum marianum, and high flavonoid content of Fagopyrum possess various interesting biological activities. The substituted pyrazine-2-carboxamides were previously used as effective elicitors of studied secondary metabolites. OBJECTIVE:To study the effect of ne...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.176117
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. MATERIALS AND METHODS:Derivatization wit...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.149735
更新日期:2015-01-01 00:00:00
abstract::Alcohol addiction is a worldwide problem. It has mainly two components: dependence and withdrawal. Characteristic properties of most anti-addictive compounds include anti-anxiety, anticonvulsant, antidepressant, and nootropic actions. Shankhpushpi (Convolvulus pluricaulis. Convolvulaceae), known ethnopharmacologically...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.203976
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Cyperus scariosus R. Br and Cyperus rotundus L are widely used in ayurvedic preparation for the treatment of diabetes and other diseases. The early literature, so far, does not indicate the presence of any bioactive principle isolated from these plants. OBJECTIVE:To identify free radical scavenging, anti-di...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.191467
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Piper trichostachyon is a wild, endemic Piper species from Western Ghats of India. The folklore healers of Belagavi region use this plant, similar to Piper nigrum. AIMS:The present study investigates the comparison between P. nigrum and P. trichostachyon using pharmacognostic parameters. MATERIALS AND METH...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.182172
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Cordyceps sinensis (CS) is a traditional Chinese medicine contains potent active metabolites such as nucleosides and polysaccharides. The submerged cultivation technique is studied for the large scale production of CS for biomass and metabolites production. OBJECTIVE:To optimize culture conditions for large...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.168946
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:The technique of microscopy has been applied for identification of Chinese materia medica (CMM) since decades. However, very few scientific publications report the combination of conventional microscopy and high performance liquid chromatography (HPLC) techniques for further application to quality assessment...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.141788
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Fissistigma polyanthum is a liane belonging to the Annonaceae family and it is one of the most important crude drugs in traditional Chinese medicine. OBJECTIVE:The objective was to describe the structural elucidation and the free-radical-scavenging activities of the isolated compounds from Fissistigma polya...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.96549
更新日期:2012-04-01 00:00:00
abstract:Background:Limonium tetragonum, a naturally salt-tolerant halophyte, has been studied recently and is of much interest to researchers due to its potent antioxidant and hepatoprotective activities. Objective:In the present study, we attempted to elucidate bioactive compounds from ethyl acetate (EtOAc) soluble fraction ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_477_16
更新日期:2017-10-01 00:00:00
abstract:Background:Asparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. This plant has been used in the prevention and effective treatment of various forms of cancers. Objective:This paper reports, for the first time, on the cytotoxicity of the methanol (MeOH) extract of the roots of A. adscendens and its so...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_136_17
更新日期:2018-01-01 00:00:00