Abstract:
BACKGROUND:Polygonum multiflorum is an important medicinal plant. Hairy roots systems obtained by transforming plant tissues with the natural genetic engineer Agrobacterium rhizogenes can produce valuable biological active substances, which have immense potential in the pharmaceutical industry. OBJECTIVE:To optimize the inductive and cultural conditions of P. multiflorum hairy roots and to identify the major active secondary metabolites in hairy roots. MATERIALS AND METHODS:P. multiflorum hairy root were mediated with A. rhizogenes R1601 to induce hairy roots. Four combinations, including Murashige-Skoog (MS), 1/2 MS, B5, and White, were investigated to optimize the culture medium. MS medium was selected for the growth measurement. The qualitative and quantitative determinations of free anthraquinone in hairy roots were compared with the calli and aseptic plantlets using high-performance liquid chromatography. RESULTS:The inductive rates of hairy roots by leaves were higher than for any other explants. The presence of agropine in the P. multiflorum hairy roots confirmed that they were indeed transgenic. MS medium was the most suitable of the four media for hairy root growth. Meanwhile, the growth kinetics and nutrient consumption results showed that the hairy roots displayed a sigmoidal growth curve and that their optimal inoculation time was 18-21 days. The determination of the anthraquinone constituents indicated that the rhein content of the hairy roots reached 2.495 μg g(-1) and was 2.55-fold higher than that of natural plants. CONCLUSION:Transgenic hairy roots of P. multiflorum could be one of the most potent materials for industrial-scale production of bioactive anthraquinone constituents.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Huang B,Lin H,Yan C,Qiu H,Qiu L,Yu Rdoi
10.4103/0973-1296.126671subject
Has Abstractpub_date
2014-01-01 00:00:00pages
77-82issue
37eissn
0973-1296issn
0976-4062pii
PM-10-77journal_volume
10pub_type
杂志文章abstract:Background:Gelatinous Chinese medicines (GCMs) including Asini Corii Colla, Testudinis Carapacis ET Plastri Colla, and Cervi Cornus Colla, were made from reptile shell or mammalian skin or deer horn, and consumed as a popular tonic, as well as hemopoietic and hemostatic agents. Misuse of them would not exert their func...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_54_17
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Grapes and their products are known to have been used for the treatment of diseases throughout history. OBJECTIVE:It was aimed to investigate the effects of Erzincan Cimin grapes on an organism model of Caenorhabditis elegans N2 wild type and C. elegans BS913 strains with gonad cancer. MATERIALS AND METHOD...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.210164
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Mangroves have the ability to grow where no other vascular plants survive. Rhizophora mucronata is a true mangrove and traditionally used to treat diabetes and its allied complications. OBJECTIVES:In the present study, we standardized the 80% methanolic standardized extract of R. mucronata leaves (RH) and f...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.153094
更新日期:2015-04-01 00:00:00
abstract:Background:Limonium tetragonum, a naturally salt-tolerant halophyte, has been studied recently and is of much interest to researchers due to its potent antioxidant and hepatoprotective activities. Objective:In the present study, we attempted to elucidate bioactive compounds from ethyl acetate (EtOAc) soluble fraction ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_477_16
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Piper trichostachyon is a wild, endemic Piper species from Western Ghats of India. The folklore healers of Belagavi region use this plant, similar to Piper nigrum. AIMS:The present study investigates the comparison between P. nigrum and P. trichostachyon using pharmacognostic parameters. MATERIALS AND METH...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.182172
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:The aim was to study and identify free radicals scavenging and antihyperglycemic principles in fruit of Piper attenuatum. MATERIALS AND METHODS:Bioassay guided identification of extracts possessing potent free radical scavenging activity, and isolation of compounds was done. Chloroform extract of P. attenuat...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.153063
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:Recently, many efforts have been made to discover new products of natural origin which can limit the xenobiotic-induced hepatic injury. Carbon tetrachloride (CCl4) is a highly toxic chemical that is widely used to study hepatotoxicity in animal models. OBJECTIVE:The present study was conducted to investigat...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.157705
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:Mimosa caesalpiniifolia Benth. (Leguminosae) is widely found in the Brazilian Northeast region and markedly contributes to production of pollen and honey, being considered an important honey plant in this region. OBJECTIVE:To investigate the chemical composition of the ethanol extract of leaves from M. caes...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.139773
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:The majority of Achillea species are the most important native economic plants of Anatolia. They include highly bioactive compounds, so they have therapeutic applications. OBJECTIVE:In the present study, the aim was to investigate in vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea tereti...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.166060
更新日期:2015-10-01 00:00:00
abstract::The fruits of eight myrtles, Myrtus communis L. accessions from the Mediterranean region of Turkey were evaluated for their antioxidant activities and fatty acid contents. The antioxidant activities of the fruit extracts were determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene-linoleic acid assa...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.59960
更新日期:2010-01-01 00:00:00
abstract:Introduction:In the present work, the method to extract, isolate, and characterize orange peel pectin using soxhlation, and thereafter, the use of this polymer-polymer in the formulation of fast dispersable and slow-releasing tablet has been studied. Thereafter, the evaluation and comparison of fast dispersible/slow-re...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_193_17
更新日期:2017-10-01 00:00:00
abstract:Background:Meyna spinosa (M.S) (Roxb.) ex Link and Oroxylum indicum (O.I) (Linn.) Vent, widely used traditional Northeast Indian medicinal plant used for various purposes, have not yet explored for safety profile. Objective:To investigate the safety profile of M.S (Roxb.) ex Link leaves and O.I (Linn.) Vent stem bark ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_62_15
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Microwave-assisted reflux extraction of polysaccharides YPF-P from the famous Chinese traditional drug, Yupingfeng powder, optimization of extracting conditions and evaluation of their antioxidant activity were conducted in this study. RESULTS:Single factor effect trends were achieved through yields and con...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.160468
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. OBJECTIVE:A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- h...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.133267
更新日期:2014-04-01 00:00:00
abstract:CONTEXT:Recent studies have demonstrated that baicalin has antihyperglycemic effects by inhibiting lipid peroxidation. Baicalin is low hydrophilic and poorly absorbed after oral administration. Thus, a suitable formulation is highly desired to overcome the disadvantages of baicalin. OBJECTIVE:The objective of this wor...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.186347
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Gegenqinlian decoction (GQD) is a famous traditional medicine recipe. It is composed of four herbs including Radix Puerariae (GG), Radix Scutellariae (HQ), Rhizoma Coptidis (HL) and Radix Glycyrrhizae (GC), which is widely used for treating gastro-intestinal disorders in the clinical practice of Traditional ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.137363
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Buddleja davidii and B. albiflora are two different original plants of the famous crude medicine "Diaoyangchen". MATERIALS AND METHODS:An ultraviolet-visible spectrophotometric method and a HPLC method were used for the determination of total and individual flavonoids (luteolin and apigenin) contents from t...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.103651
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:The purple sweet potato, Ipomoea batatas, belongs to the family Convolvulaceae. It is one of the most widely consumed tubers in Asia and is found in many dishes. Many people with diabetes eat purple sweet potato tubers to help reduce blood glucose in China. OBJECTIVE:To predict the ultrasonic conditions for...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.211044
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Annona vepretorum (AV) is a native tree from Caatinga biome (semiarid region of Brazil) popularly known as "araticum" and "pinha da Caatinga." OBJECTIVE:This study was carried out to evaluate the chemical constituents and antioxidant activity (AA) of the essential oil from the leaves from AV (EO-Av) collect...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.160462
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:The present study reports and compares the results of Gas Chromatographic-Mass analyses of Pulicaria jaubertii leaf (P-1) and root (P-2) essential oils, as well as their in vitro antimicrobial and cytotoxic activities. MATERIALS AND METHODS:The chemical composition of P-1 and P-2 essential oils of P. jauber...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.108133
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Lavatera cretica L. was used in folk medicine as anti-inflammatory among other applications. As inflammation is many times associated with oxidative processes, the aim of the present work was to evaluate the ability of aqueous extracts obtained from different parts of L. cretica to prevent oxidation or infla...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.149743
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Tephrosia purpurea is an Indian herb used in traditional medicine to treat various diseases such as jaundice, asthma, liver and urinary disorders. However, the anti-cancer potential of T. purpurea on hepatocellular carcinoma (HCC) is poorly understood. Therefore, this study aims to investigate the anti-cance...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.203981
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Traditionally, a number of medicinal plants are used to treat various types of hepatic disorders but few of them were pharmacologically evaluated for their safety and efficacy. The combination of Andrographis paniculata (Kalmegha), Tinospora cordifolia (Guduchi), and Solanum nigrum (Kakmachi) was traditional...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.168945
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVE:To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. MATERIALS AND METHODS:Derivatization wit...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.149735
更新日期:2015-01-01 00:00:00
abstract:Background:Bacopa monnieri (BM) is a herbaceous plant traditionally used from time immemorial in Ayurvedic and folklore medicines. We hypothesized that the extract of the whole plant might contain numerous molecules with having antitumor activities that could be very effective in killing of human cancer cells. Objecti...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_397_16
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Anisomeles malabarica R. Br. (Lamiaceae) is extensively used in traditional medicine in major parts of India for several medicinal purposes, including their use in rheumatism. MATERIALS AND METHODS:The air-dried leaves of A. malabarica were extracted with ethanol, defatted with n-hexane and then successivel...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.139795
更新日期:2014-08-01 00:00:00
abstract:Background:Hawthorn (Crataegus pinnatifida) fruit has enjoyed a great popularity as a pleasant-tasting food associated with hypolipidemic and antioxidant effects. Objective:Our aim was to screen the effective fraction of hawthorn fruit in the treatment of hyperlipidemia rats. Materials and Methods: In this study, etha...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_402_16
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Rattan tea is a medicinal plant that has been used for many years for the treatment of inflammation, fatty liver, tumor, diabetes, and hyperlipidemia. OBJECTIVE:A green and novel approach based on surfactant-mediated, ultrasonic-assisted extraction (SM-UAE) was developed for the extraction of antioxidant po...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_159_16
更新日期:2017-07-01 00:00:00
abstract:Background:Withania somnifera is a medicinal plant native to India and is known to have anticancer properties. It has been investigated for its anti-melanoma properties, and since melanoma presents on the skin, it is prudent to probe the use of W. somnifera in topical formulations. To enhance topical drug delivery and ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/pm.pm_489_16
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:The plant Launaea procumbens belongs to the family Asteraceae and traditionally used in the treatment rheumatism, kidney, liver dysfunctions and eye diseases. In the present study Phytochemical analysis and fractions of methanolic extract of L. procumbens leaves were tested in vitro for their cytotoxicity. ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.191452
更新日期:2016-07-01 00:00:00