Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection.

Abstract:

BACKGROUND:Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. OBJECTIVE:A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- hydroxymethylfurfural, mangiferin, paeoniflorin, nodakenin, naringin, hesperidin, decursinol, berberine, glycyrrhizin, atractylenolide III and decursin, in JEGH. MATERIALS AND METHODS:The separation was conducted on Shishedo C18 column with gradient elution of 0.1% trifluoroacetic acid-acetonitrile. Detection of wavelength was set at 205, 250, 280 and 330 nm. RESULTS:The developed analysis showed a good linearity (R (2) >0.9997). The range of limit of detection and limit of quantification were observed from 0.04 to 0.43 and from 0.11 to 1.30, respectively. The intra- and inter-day test relative standard deviations (RSD) were less than 3% and the accuracy was 95.98-108.44%. The recoveries were between 92.75% and 109.19% and RSD range of recoveries was measured from 0.52% to 2.78%. CONCLUSION:This HPLC-DAD method can be successfully applied for simultaneous determination of 11 major compounds in JEGH samples.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Yun BR,Weon JB,Lee J,Eom MR,Ma CJ

doi

10.4103/0973-1296.133267

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

S256-63

issue

Suppl 2

eissn

0973-1296

issn

0976-4062

pii

PM-10-256

journal_volume

10

pub_type

杂志文章