Abstract:
Background:Bacopa monnieri (BM) is a herbaceous plant traditionally used from time immemorial in Ayurvedic and folklore medicines. We hypothesized that the extract of the whole plant might contain numerous molecules with having antitumor activities that could be very effective in killing of human cancer cells. Objectives:This work investigated anticancer activity of bioactive fraction of BM. Materials and Methods:The hydroalcoholic extract of BM was fractionated with different solvent, namely, hexane, dichloromethane (DCM), acetone, methanol, and water. The in vitro anticancer activity was performed against various Human Cancer Cell lines, namely, Colon (HT29, Colo320, and Caco2), Lung (A549), Cervix (HeLa, SiHa), and Breast (MCF-7, MDAMB-231). Further, DCM fraction was evaluated in vivo for anticancer activity against Ehrlich ascites carcinoma (EAC) tumor-bearing mice since it showed the best cytotoxicity at 72 h (IC50 41.0-60.0 µg/mL). The metabolic fingerprinting of these extract were carried out using high-performance thin-layer chromatography along with quantification of bacoside A, bacoside B, cucurbitacin B, cucurbitacin E, and bittulinic acid. Results:Oral administration of DCM fraction at a dose of 40 mg/kg rendered prominent reduction of tumor regression parameters such as tumor weight, packed cell volume, tumor volume and viable tumor cell count as compared to the untreated mice of the EAC control group. The anticancer activity of DCM fraction may be due to the presence of large amount of bacoside A, B and cucurbitacins. The molecular docking studies of major metabolites with targeted proteins predicted the anticancer activity of DCM fraction which was in support of in vivo activity. Conclusion:The in vitro, in vivo, analytical and in silico studies on DCM fraction of Bacopa monieri has proved its great potential for development of anticancer phytopharmaceuticals. SUMMARY:A new HPTLC method has been developed and validated for the qualitative and quantitative analysis of bacoside A, B, cucurbitacin B, D, E and bittulinic acid in Bacopa monnieri extract. Enrichment of active anticancer metabolites was done by polarity based fractionations of hydroalcoholic extract of Bacopa. DCM fraction of a hydroalcoholic extract of Bacopa showed anticancer potential against human cancer cell line (IC50 41.0-60.0 µg/mL) and in EAC treated mice (at a dose of 40 mg/kg body weight). The anticancer activity of Bacopa may be due to the presence of bacosides and cucurbitacin and it was confirmed by in silico screening. Abbreviations used: DBM: DCM fraction of Bacopa monnieri; DCM: Dichloromethane; EAC: Ehrlich ascites carcinoma; HCT: Hematocrit; HGB: Hemoglobin; HPTLC: High performance thin layer chromatography; ICH: International council for Harmonisation; LOD: Limit of detection; LOQ: Limit of quantification; LYM: Lymphocytes; MCH: Mean corpuscular hemoglobin; MCHC: Mean corpuscular haemoglobin concentration (MCHC); MCV: Mean corpuscular volume; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PLT: Platelet; RBC: Red blood cell; RDW: Red blood cell distribution width; RSD: Relative standard deviation; WBC: White blood cells.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Mallick MN,Khan W,Parveen R,Ahmad S,Sadaf,Najm MZ,Ahmad I,Husain SAdoi
10.4103/pm.pm_397_16subject
Has Abstractpub_date
2017-10-01 00:00:00pages
S595-S606issue
Suppl 3eissn
0973-1296issn
0976-4062pii
PM-13-595journal_volume
13pub_type
杂志文章abstract:BACKGROUND:The plant Launaea procumbens belongs to the family Asteraceae and traditionally used in the treatment rheumatism, kidney, liver dysfunctions and eye diseases. In the present study Phytochemical analysis and fractions of methanolic extract of L. procumbens leaves were tested in vitro for their cytotoxicity. ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.191452
更新日期:2016-07-01 00:00:00
abstract::The fruits of eight myrtles, Myrtus communis L. accessions from the Mediterranean region of Turkey were evaluated for their antioxidant activities and fatty acid contents. The antioxidant activities of the fruit extracts were determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene-linoleic acid assa...
journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.59960
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journal_title:Pharmacognosy magazine
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更新日期:2013-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2014-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.204548
更新日期:2017-04-01 00:00:00
abstract::Alcohol addiction is a worldwide problem. It has mainly two components: dependence and withdrawal. Characteristic properties of most anti-addictive compounds include anti-anxiety, anticonvulsant, antidepressant, and nootropic actions. Shankhpushpi (Convolvulus pluricaulis. Convolvulaceae), known ethnopharmacologically...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.203976
更新日期:2017-01-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2014-08-01 00:00:00
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更新日期:2011-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2017-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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journal_title:Pharmacognosy magazine
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更新日期:2015-05-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2012-04-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.137363
更新日期:2014-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2017-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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更新日期:2015-04-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Haberlea rhodopensis Friv. (Gesneriaceae) is a rare poikilohydric endemic and preglacial relict growing in Balkan Peninsula. Previous investigations demonstrated strong antioxidant, antimicrobial and antimutagenic potential of alcoholic extract from the plant. OBJECTIVE:The isolation of known caffeoyl pheny...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.117822
更新日期:2013-10-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Plant sterols are the major source of micronutrients and have not shown any obvious side effects in human. β-sitosterol is one of the most prevalent phytosterols which have been recorded in ancient medicinal history for its use in the treatment of many chronic diseases, especially cancer. The modulations of ...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.197634
更新日期:2017-01-01 00:00:00
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更新日期:2017-07-01 00:00:00
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更新日期:2015-04-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:The technique of microscopy has been applied for identification of Chinese materia medica (CMM) since decades. However, very few scientific publications report the combination of conventional microscopy and high performance liquid chromatography (HPLC) techniques for further application to quality assessment...
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更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication. OBJECTIVE:To establish and optimize the rapid propagation technology of S. flavesce...
journal_title:Pharmacognosy magazine
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更新日期:2010-10-01 00:00:00
abstract::Mentha piperita essential oil was bactericidal in order of E. coli> S. aureus > Pseudomonas aeruginosa > S. faecalis > Klebsiella pneumoniae. The oil with total phenolics of 89.43 ± 0.58 µg GAE/mg had 63.82 ± 0.05% DPPH inhibition activity with an IC (50) = 3.9 µg/ml. Lipid peroxidation inhibition was comparable to BH...
journal_title:Pharmacognosy magazine
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00