Chemical constituents and toxicological studies of leaves from Mimosa caesalpiniifolia Benth., a Brazilian honey plant.

Abstract:

BACKGROUND:Mimosa caesalpiniifolia Benth. (Leguminosae) is widely found in the Brazilian Northeast region and markedly contributes to production of pollen and honey, being considered an important honey plant in this region. OBJECTIVE:To investigate the chemical composition of the ethanol extract of leaves from M. caesalpiniifolia by GC-MS after derivatization (silylation), as well as to evaluate the in vitro and in vivo toxicological effects and androgenic activity in rats. MATERIALS AND METHODS:The ethanol extract of leaves from Mimosa caesalpiniifolia was submitted to derivatization by silylation and analyzed by gas chromatography-mass spectrometry (GC-MS) to identification of chemical constituents. In vitro toxicological evaluation was performed by MTT assay in murine macrophages and by Artemia salina lethality assay, and the in vivo acute oral toxicity and androgenic evaluation in rats. RESULTS:Totally, 32 components were detected: Phytol-TMS (11.66%), lactic acid-2TMS (9.16%), α-tocopherol-TMS (7.34%) and β-sitosterol-TMS (6.80%) were the major constituents. At the concentrations analyzed, the ethanol extract showed low cytotoxicity against brine shrimp (Artemia salina) and murine macrophages. In addition, the extract did not exhibit any toxicological effect or androgenic activity in rats. CONCLUSIONS:The derivatization by silylation allowed a rapid identification of chemical compounds from the M. caesalpiniifolia leaves extract. Besides, this species presents a good safety profile as observed in toxicological studies, and possess a great potential in the production of herbal medicines or as for food consumption.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Monção NB,Costa LM,Arcanjo DD,Araújo BQ,Lustosa Mdo C,Rodrigues KA,Carvalho FA,Costa AP,Lopes Citó AM

doi

10.4103/0973-1296.139773

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

S456-62

issue

Suppl 3

eissn

0973-1296

issn

0976-4062

pii

PM-10-456

journal_volume

10

pub_type

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