Abstract:
BACKGROUND:Coffea canephora is the commonly cultivated coffee species in the world along with Coffea arabica. Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particularly against insect pests. The key enzymes involved in the production of JA are lipoxygenase, allene oxide synthase, allene oxide cyclase, and 12-oxo-phytodienoic reductase. There is no report on the genes involved in JA pathway in coffee plants. OBJECTIVE:We made an attempt to identify and analyze the genes coding for these enzymes in C. canephora. MATERIALS AND METHODS:First, protein sequences of jasmonate pathway genes from model plant Arabidopsis thaliana were identified in the National Center for Biotechnology Information (NCBI) database. These protein sequences were used to search the web-based database Coffee Genome Hub to identify homologous protein sequences in C. canephora genome using Basic Local Alignment Search Tool (BLAST). RESULTS:Homologous protein sequences for key genes were identified in the C. canephora genome database. Protein sequences of the top matches were in turn used to search in NCBI database using BLAST tool to confirm the identity of the selected proteins and to identify closely related genes in species. The protein sequences from C. canephora database and the top matches in NCBI were aligned, and phylogenetic trees were constructed using MEGA6 software and identified the genetic distance of the respective genes. The study identified the four key genes of JA pathway in C. canephora, confirming the conserved nature of the pathway in coffee. The study expected to be useful to further explore the defense mechanisms of coffee plants. CONCLUSION:JA is a plant hormone that plays an important role in plant defense against insect pests. Genes coding for the 4 key enzymes involved in the production of JA viz., LOX, AOS, AOC, and OPR are identified in C. canephora (robusta coffee) by bioinformatic approaches confirming the conserved nature of the pathway in coffee. The findings are useful to understand the defense mechanisms of C. canephora and coffee breeding in the long run. SUMMARY:JA is a plant hormone that plays an important role in plant defense against insect pests. Genes coding for the 4 key enzymes involved in the production of JA viz., LOX, AOS, AOC and OPR were identified and analyzed in C. canephora (robusta coffee) by in silico approach. The study has confirmed the conserved nature of JA pathway in coffee; the findings are useful to further explore the defense mechanisms of coffee plants. Abbreviations used:C. canephora: Coffea canephora; C. arabica: Coffea arabica; JA: Jasmonic acid; CGH: Coffee Genome Hub; NCBI: National Centre for Biotechnology Information; BLAST: Basic Local Alignment Search Tool; A. thaliana: Arabidopsis thaliana; LOX: Lipoxygenase, AOS: Allene oxide synthase; AOC: Allene oxide cyclase; OPR: 12 oxo phytodienoic reductase.
journal_name
Pharmacogn Magjournal_title
Pharmacognosy magazineauthors
Bharathi K,Sreenath HLdoi
10.4103/pm.pm_518_16subject
Has Abstractpub_date
2017-07-01 00:00:00pages
S196-S200issue
Suppl 2eissn
0973-1296issn
0976-4062pii
PM-13-196journal_volume
13pub_type
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.211044
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doi:10.4103/0973-1296.168946
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abstract:BACKGROUND:The RP-HPLC resolution of two triterpenic acid isomers was unstable. OBJECTIVE:To separate the oleanolic acid (OA) and ursolic acid (UA) simply within RP-HPLC. MATERIALS AND METHODS:The separation ability of five stationary phases was studied with the retention effect of their carbon loads. Also the resolu...
journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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doi:10.4103/0973-1296.191467
更新日期:2016-07-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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更新日期:2014-07-01 00:00:00
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journal_title:Pharmacognosy magazine
pub_type: 杂志文章
doi:10.4103/0973-1296.139795
更新日期:2014-08-01 00:00:00
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journal_title:Pharmacognosy magazine
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journal_title:Pharmacognosy magazine
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更新日期:2016-01-01 00:00:00
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journal_title:Pharmacognosy magazine
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更新日期:2014-04-01 00:00:00
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更新日期:2018-04-01 00:00:00
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更新日期:2015-10-01 00:00:00