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Microwave-assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity.

Abstract：

BACKGROUND:Microwave-assisted reflux extraction of polysaccharides YPF-P from the famous Chinese traditional drug, Yupingfeng powder, optimization of extracting conditions and evaluation of their antioxidant activity were conducted in this study. RESULTS:Single factor effect trends were achieved through yields and contends of YPF-P obtained from different extracting conditions. Then through a three-level, four-variable Box-Behnken design of response surface methodology adopting yield as response, the optimal conditions were determined as follows: Material/solvent ratio 1:23.37, microwave power 560 W, Extraction temperature 64°C, and extraction time 9.62 min. Under the optimal conditions, the YPF-P extraction yield was 3.23%, and its content was detected as 38.52%. In antioxidant assays, the YPF-P was tested to possess 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities with an IC50 value of 0.262 mg/ml. In addition, YPF-P was also proved to have relatively low ferric reducing antioxidant power (FRAP), compared to Vc, through FRAP assay. CONCLUSION:In the microwave assisted reflux extraction research, good YPF-P yield was achieved from materials with relatively low YPF-P content. And for the first time, both DPPH and FRAP assays were conducted on YPF-P, which proved that the antioxidant activity of YPF-P contributed to the functions of this medicine.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Wang D,Zhang BB,Qu XX,Gao F,Yuan MY

doi

10.4103/0973-1296.160468

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

546-54

issue

43

eissn

0973-1296

issn

0976-4062

pii

PM-11-546

journal_volume

11

pub_type

杂志文章

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