In vitro Anti-Proliferative Effect of Tephrosia purpurea on Human Hepatocellular Carcinoma Cells.


BACKGROUND:Tephrosia purpurea is an Indian herb used in traditional medicine to treat various diseases such as jaundice, asthma, liver and urinary disorders. However, the anti-cancer potential of T. purpurea on hepatocellular carcinoma (HCC) is poorly understood. Therefore, this study aims to investigate the anti-cancer activity of T. purpurea in HepG2 hepatocellular carcinoma cells. METHODS:The leaves and root of T. purpurea were extracted with methanol using soxhlet apparatus. The cytotoxicity of the T. purpurea extracts in HepG2 cells was evaluated using MTT assay whereas the mode of cell death was examined by AOEB, Hoechst and JC1 staining under a fluorescence microscope. T. purpurea extracts-induced caspase-3 expression was investigated using colorimetric assay. RESULTS:The leaves and root extracts inhibited HepG2 cell growth at the IC50 of 102.33 ± 10.26 µg/mL and 276.67 ± 20.43 µg/mL respectively at 24 h. Chromatin condensation, nuclear fragmentation, apoptotic bodies formation and mitochondrial membrane depolarization were observed in HepG2 cells treated with both extracts. The caspase-3 expression was significantly (p < 0.05) increased in extracts treated cells when compared to control. CONCLUSION:The leaves and root extracts of T. purpurea induce apoptosis mediated cell death in HepG2 cells. SUMMARY:The leaves and root extracts of T. purpurea exhibited anticancer activity in HepG2 hepatocellular carcinoma cells. These extracts induced cell shrinkage, DNA condensation and fragmentation, mitochondrial membrane depolarization and upregulated caspase-3 expression indicating T. purpurea extracts induce apoptosis in HepG2 cells. Abbreviation used: AO: acridine orange, DMSO: dimethyl sulfoxide, EB: ethidium bromide, IC50: the concentration at which 50% of cancer cells are dead, JC-1: 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetraethyl-imidacarbocyanine iodide, MTT: 3-4, 5-dimethylthiazole-2-yl, 2,5-diphenyl tetrazolium bromide, PBS: phosphate-buffered saline, ΔΨm: mitochondrial trans-membrane potential.


Pharmacogn Mag


Pharmacognosy magazine


Padmapriya R,Gayathri L,Ronsard L,Akbarsha MA,Raveendran R




Has Abstract


2017-01-01 00:00:00




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  • Comparative Proteomic Analysis of Three Gelatinous Chinese Medicines and Their Authentications by Tryptic-digested Peptides Profiling using Matrix-assisted Laser Desorption/Ionization-time of Flight/Time of Flight Mass Spectrometry.

    abstract:Background:Gelatinous Chinese medicines (GCMs) including Asini Corii Colla, Testudinis Carapacis ET Plastri Colla, and Cervi Cornus Colla, were made from reptile shell or mammalian skin or deer horn, and consumed as a popular tonic, as well as hemopoietic and hemostatic agents. Misuse of them would not exert their func...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Yang H,Zheng J,Wang HY,Li N,Yang YY,Shen YP

    更新日期:2017-10-01 00:00:00

  • Analysis and comparison of the active components and antioxidant activities of extracts from Abelmoschus esculentus L.

    abstract:BACKGROUND:Abelmoschus esculentus L. is a healthy vegetable belonging to the family Malvaceae. This article reports the contents of total phenolics (TP) and total flavonoids (TF) in 80% methanol extracts of the flower (FL), fruit (FR), leaf (L), and seed (S) of A. esculentus, and in 0, 10, 30, 50, and 70% methanol elua...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Liao H,Dong W,Shi X,Liu H,Yuan K

    更新日期:2012-04-01 00:00:00

  • Chemical composition and resistance-modifying effect of the essential oil of Lantana camara Linn.

    abstract::In this work, the chemical constituents, antibacterial and modulatory activities of the essential oil of Lantana camara Linn were studied. The essential oil was extracted from the leaves of L. camara by hydrodistillation method using Clevenger's apparatus and its chemical constituents were separated and identified by ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Sousa EO,Silva NF,Rodrigues FF,Campos AR,Lima SG,Costa JG

    更新日期:2010-04-01 00:00:00

  • Neuroprotective activity of the methanolic extract of Lonicera japonica in glutamate-injured primary rat cortical cells.

    abstract:BACKGROUND:We previously reported that the extracts of several Korean medicinal plants showed neuroprotective activity in glutamate-injured primary culutres of rat cortical cells. OBJECTIVE:Among them, the effect of the methanolic extract of Lonicera japonica flower on the glutamate-induced neuronal cell death and its...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Weon JB,Yang HJ,Lee B,Yun BR,Ahn JH,Lee HY,Ma CJ

    更新日期:2011-10-01 00:00:00

  • Evaluation of Hypolipidemic and Antioxidant Effects in Phenolrich Fraction of Crataegus pinnatifida Fruit in Hyperlipidemia Rats and Identification of Chemical Composition by Ultra-performance Liquid Chromatography Coupled with Quadropole Time-of-flight M

    abstract:Background:Hawthorn (Crataegus pinnatifida) fruit has enjoyed a great popularity as a pleasant-tasting food associated with hypolipidemic and antioxidant effects. Objective:Our aim was to screen the effective fraction of hawthorn fruit in the treatment of hyperlipidemia rats. Materials and Methods: In this study, etha...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Shao F,Gu L,Chen H,Liu R,Huang H,Chen L,Yang M

    更新日期:2017-10-01 00:00:00

  • Phytoecdysteroids of the East Asian Caryophyllaceae.

    abstract:BACKGROUND:Occurrence of integristerone A (1), 20-hydroxyecdysone (2), ecdysone (3), 2-deoxy-20-hydroxyecdysone (4) has been analyzed in 64 species of the East Asian Caryophyllaceae. MATERIALS AND METHODS:Ecdysteroid content was determinate by high-performance liquid chromatography (HPLC). HPLC with a high-resolution ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Novozhilova E,Rybin V,Gorovoy P,Gavrilenko I,Doudkin R

    更新日期:2015-05-01 00:00:00

  • Comparison of pharmacokinetic behavior of two iridoid glycosides in rat plasma after oral administration of crude Cornus officinals and its jiuzhipin by high performance liquid chromatography triple quadrupole mass spectrometry combined with multiple reac

    abstract:OBJECTIVE:The present study examined the pharmacokinetic profiles of two iridoid glycosides named morroniside and loganin in rat plasma after oral administration of crude and processed Cornus officinals. MATERIALS AND METHODS:A rapid, selective and specific high-performance liquid chromatography/electrospray ionizatio...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Chen X,Cao G,Jiang J

    更新日期:2014-01-01 00:00:00

  • Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora.

    abstract:BACKGROUND:Zataria multiflora Boiss. is a traditional and popular spice in Iran. The effects of 3 ecotypes (ECTPs) of Z. multiflora essential oils (EOs) against most common causes of food-borne and nosocomial infections were evaluated. MATERIALS AND METHODS:The antimicrobial activities of the EOs were examined by brot...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Zomorodian K,Saharkhiz MJ,Rahimi MJ,Bandegi A,Shekarkhar G,Bandegani A,Pakshir K,Bazargani A

    更新日期:2011-01-01 00:00:00

  • Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection.

    abstract:BACKGROUND:Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. OBJECTIVE:A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- h...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Yun BR,Weon JB,Lee J,Eom MR,Ma CJ

    更新日期:2014-04-01 00:00:00

  • Exploring the Cytotoxic Potential of Triterpenoids-enriched Fraction of Bacopa monnieri by Implementing In vitro, In vivo, and In silico Approaches.

    abstract:Background:Bacopa monnieri (BM) is a herbaceous plant traditionally used from time immemorial in Ayurvedic and folklore medicines. We hypothesized that the extract of the whole plant might contain numerous molecules with having antitumor activities that could be very effective in killing of human cancer cells. Objecti...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Mallick MN,Khan W,Parveen R,Ahmad S,Sadaf,Najm MZ,Ahmad I,Husain SA

    更新日期:2017-10-01 00:00:00

  • Induction of Apoptosis and Cell Cycle Arrest by Flavokawain C on HT-29 Human Colon Adenocarcinoma via Enhancement of Reactive Oxygen Species Generation, Upregulation of p21, p27, and GADD153, and Inactivation of Inhibitor of Apoptosis Proteins.

    abstract::Chalcones have been shown to exhibit anti-cancer properties by targeting multiple molecular pathways. It was, therefore, of interest to investigate flavokawain C (FKC), a naturally occurring chalcone, which can be isolated from Kava (Piper methysticum Forst) root extract. The aim of this study was to investigate the i...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Phang CW,Karsani SA,Abd Malek SN

    更新日期:2017-07-01 00:00:00

  • Effects of Pogostemon cablin Blanco extract on hypoxia induced rabbit cardiomyocyte injury.

    abstract:BACKGROUND:Pogostemonis Herba, the dried aerial part of Pogostemon cablin Blanco, is a well-known materia medica in Asia that is widely used for syndrome of gastrointestinal dysfunctions. OBJECTIVE:This study was undertaken to examine whether Pogostemon cablin extract (PCe) might have any beneficial effect on hypoxia ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Lim CY,Kim BY,Lim SH,Cho SI

    更新日期:2015-04-01 00:00:00

  • Chemical composition and biological evaluation of essential oils of Pulicaria jaubertii.

    abstract:BACKGROUND:The present study reports and compares the results of Gas Chromatographic-Mass analyses of Pulicaria jaubertii leaf (P-1) and root (P-2) essential oils, as well as their in vitro antimicrobial and cytotoxic activities. MATERIALS AND METHODS:The chemical composition of P-1 and P-2 essential oils of P. jauber...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Fawzy GA,Al Ati HY,El Gamal AA

    更新日期:2013-01-01 00:00:00

  • Pharmacokinetics, intestinal absorption and microbial metabolism of single platycodin D in comparison to Platycodi radix extract.

    abstract:BACKGROUND:Platycodi radix, the dried root of Platycodon grandiflorum A. DC, has been widely used as food and herb medicine for treating cough, cold and other respiratory ailments, and platycodin D (PD) is one of the most important compounds in Platycodi Radix. OBJECTIVE:The purpose of this study was to compare the ph...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Shan J,Zou J,Xie T,Kang A,Zhou W,Deng H,Mao Y,Di L,Wang S

    更新日期:2015-10-01 00:00:00

  • Application of microscopy technique and high performance liquid chromatography for quality assessment of Polygonum multiflorum Thunb. (Heshouwu).

    abstract:BACKGROUND:The technique of microscopy has been applied for identification of Chinese materia medica (CMM) since decades. However, very few scientific publications report the combination of conventional microscopy and high performance liquid chromatography (HPLC) techniques for further application to quality assessment...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Liang L,Zhao Z,Kang T

    更新日期:2014-10-01 00:00:00

  • Near-infrared Spectroscopy as a Process Analytical Technology Tool for Monitoring the Parching Process of Traditional Chinese Medicine Based on Two Kinds of Chemical Indicators.

    abstract:BACKGROUND:The active ingredients and thus pharmacological efficacy of traditional Chinese medicine (TCM) at different degrees of parching process vary greatly. OBJECTIVE:Near-infrared spectroscopy (NIR) was used to develop a new method for rapid online analysis of TCM parching process, using two kinds of chemical ind...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Li K,Wang W,Liu Y,Jiang S,Huang G,Ye L

    更新日期:2017-04-01 00:00:00

  • Topical Delivery of Withania somnifera Crude Extracts in Niosomes and Solid Lipid Nanoparticles.

    abstract:Background:Withania somnifera is a medicinal plant native to India and is known to have anticancer properties. It has been investigated for its anti-melanoma properties, and since melanoma presents on the skin, it is prudent to probe the use of W. somnifera in topical formulations. To enhance topical drug delivery and ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Chinembiri TN,Gerber M,du Plessis LH,du Preez JL,Hamman JH,du Plessis J

    更新日期:2017-10-01 00:00:00

  • Effects of Aronia melanocarpa Fruit Juice on Isolated Rat Hepatocytes.

    abstract:BACKGROUND:Aronia melanocarpa (Michx.) Elliot fruits are very rich in polyphenols - procyanidins, flavonoids, and phenolic acids. OBJECTIVE:On rat hepatocytes, isolated by two-stepped collagenase perfusion, we investigated the effect of A. melanocarpa fruit juice (AMFJ) in two models of liver toxicity caused by (i) me...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Kondeva-Burdina M,Valcheva-Kuzmanova S,Markova T,Mitcheva M,Belcheva A

    更新日期:2015-10-01 00:00:00

  • Quantitative determination of total and individual flavonoids in stems and leaves of Buddleja davidii and Buddleja albiflora.

    abstract:BACKGROUND:Buddleja davidii and B. albiflora are two different original plants of the famous crude medicine "Diaoyangchen". MATERIALS AND METHODS:An ultraviolet-visible spectrophotometric method and a HPLC method were used for the determination of total and individual flavonoids (luteolin and apigenin) contents from t...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Ying C,Wan D

    更新日期:2012-10-01 00:00:00

  • 7-hydroxycalamenene Effects on Secreted Aspartic Proteases Activity and Biofilm Formation of Candida spp.

    abstract:BACKGROUND:The 7-hydroxycalamenenene-rich essential oil (EO) obtained from the leaves of Croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. In this work, we aimed to evaluate the effectiveness of 7-hydroxycalamenenene against Candida albicans and nonalbicans sp...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Azevedo MM,Almeida CA,Chaves FC,Rodrigues IA,Bizzo HR,Alviano CS,Alviano DS

    更新日期:2016-01-01 00:00:00

  • Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts.

    abstract:BACKGROUND:Hakea sericea Schrader is an invasive shrub in Portuguese forests. OBJECTIVE:The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Luís A,Breitenfeld L,Ferreira S,Duarte AP,Domingues F

    更新日期:2014-01-01 00:00:00

  • New ionic liquid-based preparative method for diosgenin from Rhizoma dioscoreae nipponicae.

    abstract:BACKGROUND:Rhizoma dioscoreae nipponicae is a perennial herb and its roots have been widely used in Traditional Chinese Medicine (TCM). OBJECTIVE:To develop and optimize the extraction and hydrolysis technology of diosgenin from Rhizoma dioscoreae nipponicae. MATERIALS AND METHODS:1-methyl-3-(3-sulfopropyl)-imidazoli...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Yan W,Ji L,Hang S,Shun Y

    更新日期:2013-07-01 00:00:00

  • Safety Profile Investigations of Meyna spinosa (Roxb.) and Oroxylum indicum (Linn.) Extracts Collected from Northeast India.

    abstract:Background:Meyna spinosa (M.S) (Roxb.) ex Link and Oroxylum indicum (O.I) (Linn.) Vent, widely used traditional Northeast Indian medicinal plant used for various purposes, have not yet explored for safety profile. Objective:To investigate the safety profile of M.S (Roxb.) ex Link leaves and O.I (Linn.) Vent stem bark ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Singh S,Chattopadhyay P,Borthakur SK,Policegoudra R

    更新日期:2018-01-01 00:00:00

  • Sterols from the red algae, Gracilaria salicornia and Hypnea flagelliformis, from Persian Gulf.

    abstract:CONTEXT:Two of the important algae from Persian Gulf are Gracilaria salicornia and Hypnea flageliformis (Rhodophyta). Antibacterial, antifungal, and cytotoxic effects of the mentioned algae have been presented in the previous studies. AIM:In this study, the isolation and structural elucidation of the sterols from thes...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Nasir M,Saeidnia S,Mashinchian-Moradi A,Gohari AR

    更新日期:2011-04-01 00:00:00

  • Chromatographic method for determination of the free amino acid content of chamomile flowers.

    abstract:OBJECTIVE:To determine the free amino acid contents of chamomile flowers using reverse-phase high-performance column chromatography preceded by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. MATERIALS AND METHODS:Derivatization wit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Ma X,Zhao D,Li X,Meng L

    更新日期:2015-01-01 00:00:00

  • Antihyperglycemic effects of Pandanus amaryllifolius Roxb. leaf extract.

    abstract:BACKGROUND:Diabetes mellitus is one of the leading chronic diseases worldwide. In patients with poor glycemic control, high blood glucose level may lead to other life-threatening complications. Pandanus amaryllifolius Roxb. (PA) leaves are used in traditional medicine for the treatment of diabetes. OBJECTIVE:This stud...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Chiabchalard A,Nooron N

    更新日期:2015-01-01 00:00:00

  • Cytoprotective and antioxidant effects of phenolic compounds from Haberlea rhodopensis Friv. (Gesneriaceae).

    abstract:BACKGROUND:Haberlea rhodopensis Friv. (Gesneriaceae) is a rare poikilohydric endemic and preglacial relict growing in Balkan Peninsula. Previous investigations demonstrated strong antioxidant, antimicrobial and antimutagenic potential of alcoholic extract from the plant. OBJECTIVE:The isolation of known caffeoyl pheny...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Kondeva-Burdina M,Zheleva-Dimitrova D,Nedialkov P,Girreser U,Mitcheva M

    更新日期:2013-10-01 00:00:00

  • Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in A549 human lung cancer cells.

    abstract:BACKGROUND:Methyl protodioscin (MPD) is a furostanol bisglycoside with antitumor properties. It has been shown to reduce proliferation, cause cell cycle arrest. OBJECTIVE:The present study elucidates the mechanism underlying MPD's apoptotic effects, using the A549 human lung cancer cell line. MATERIALS AND METHODS:Th...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Bai Y,Qu XY,Yin JQ,Wu L,Jiang H,Long HW,Jia Q

    更新日期:2014-07-01 00:00:00

  • Increased acetylcholine esterase activity produced by the administration of an aqueous extract of the seed kernel of Thevetia peruviana and its role on acute and subchronic intoxication in mice.

    abstract:BACKGROUND:The real mechanism for Thevetia peruviana poisoning remains unclear. Cholinergic activity is important for cardiac function regulation, however, the effect of T. peruviana on cholinergic activity is not well-known. OBJECTIVE:To study the effect of the acute administration of an aqueous extract of the seed k...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Marroquín-Segura R,Calvillo-Esparza R,Mora-Guevara JL,Tovalín-Ahumada JH,Aguilar-Contreras A,Hernández-Abad VJ

    更新日期:2014-01-01 00:00:00

  • Phytoconstituents from Alpinia purpurata and their in vitro inhibitory activity against Mycobacterium tuberculosis.

    abstract::Alpinia purpurata or red ginger was studied for its phytochemical constituents as part of our growing interest on Philippine Zingiberaceae plants that may exhibit antimycobacterial activity. The hexane and dichloromethane subextracts of the leaves were fractionated and purified using silica gel chromatography to affor...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章


    authors: Villaflores OB,Macabeo AP,Gehle D,Krohn K,Franzblau SG,Aguinaldo AM

    更新日期:2010-10-01 00:00:00