Effects of Pogostemon cablin Blanco extract on hypoxia induced rabbit cardiomyocyte injury.

Abstract:

BACKGROUND:Pogostemonis Herba, the dried aerial part of Pogostemon cablin Blanco, is a well-known materia medica in Asia that is widely used for syndrome of gastrointestinal dysfunctions. OBJECTIVE:This study was undertaken to examine whether Pogostemon cablin extract (PCe) might have any beneficial effect on hypoxia induced rabbit cardiomyocyte injury. MATERIALS AND METHODS:Isolated cardiomyocytes were divided into three groups and the changes of cell viability in cardiomyocytes of hypoxic and hypoxia/reoxygenation group were determined. The effect of PCe on reactive oxygen species (ROS) generation, intracellular formation of ROS was also measured by monitoring the 2',7'-dichlorofluorescein fluorescence. RESULTS:PCe effectively protected the cells against both the hypoxia and reoxygenation induced injury, and the protective effect of PCe is not mediated by interaction with adenosine triphosphate-sensitive K(+) channels. In the presence of PCe, production of ROS under chemical hypoxia was remarkably reduced which suggests that PCe might exert its effect as a ROS scavenger. CONCLUSION:The present study provides clear evidence for the beneficial effect of PCe on cardiomyocyte injury during hypoxia or reoxygenation following prolonged hypoxia.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Lim CY,Kim BY,Lim SH,Cho SI

doi

10.4103/0973-1296.153084

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

311-9

issue

42

eissn

0973-1296

issn

0976-4062

pii

PM-11-311

journal_volume

11

pub_type

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