Abstract：
:The ability to measure biomolecular dynamics within cells and tissues is very important to understand fundamental physiological processes including cell adhesion, signalling, movement, division or metabolism. Usually, such information is obtained using particle tracking methods or single point fluctuation spectroscopy. We show that image mean square displacement analysis, applied to single plane illumination microscopy data, is a faster and more efficient way of unravelling rapid, three-dimensional molecular transport and interaction within living cells. From a stack of camera images recorded in seconds, the type of dynamics such as free diffusion, flow or binding can be identified and quantified without being limited by current camera frame rates. Also, light exposure levels are very low and the image mean square displacement method does not require calibration of the microscope point spread function. To demonstrate the advantages of our approach, we quantified the dynamics of several different proteins in the cyto- and nucleoplasm of living cells. For example, from a single measurement, we were able to determine the diffusion coefficient of free clathrin molecules as well as the transport velocity of clathrin-coated vesicles involved in endocytosis. Used in conjunction with dual view detection, we further show how protein-protein interactions can be quantified.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hedde PN,Stakic M,Gratton Edoi
10.1038/srep07048subject
Has Abstractpub_date
2014-11-14 00:00:00pages
7048issn
2045-2322pii
srep07048journal_volume
4pub_type
杂志文章abstract：:The experimental study deals with the investigation of the effect of diverse crystallinity of imidazolium ionic-liquid-based SPE on conductivity and current fluctuations. The experimental study was carried out on samples consisting of [EMIM][TFSI] as ionic liquid, PVDF as a polymer matrix and NMP as a solvent. After t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78363-6
更新日期：2020-12-03 00:00:00
abstract：:Erythropoietin (EPO) has been identified as being crucial for obesity modulation; however, its erythropoietic activity may limit its clinical application. EPO-derived Helix B-surface peptide (pHBSP) is nonerythrogenic but has been reported to retain other functions of EPO. The current study aimed to evaluate the effec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15134
更新日期：2015-10-13 00:00:00
abstract：:A bioresorbable metallic helical stent was explored as a new device opportunity (magnesium scaffold), which can be absorbed by the body without leaving a trace and simultaneously allowing restoration of vasoreactivity with the potential for vessel remodeling. In this study, developed Mg-based helical stent was inserte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01214-4
更新日期：2017-04-26 00:00:00
abstract：:Herein, water-oxidation reaction by cobalt(II) phthalocyanine, N,N'-bis (salicylidene) ethylenediamino cobalt(II), nickel(II) Schiff base (N,N'-bis (salicylidene)ethylenediamino nickel(II), nickel(II)) phthalocyanine-tetrasulfonate tetrasodium, manganese(II) phthalocyanine, 5,10,15,20-Tetraphenyl-21H,23H-porphine mang...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43953-6
更新日期：2019-05-16 00:00:00
abstract：:Working memory capacity is incredibly limited and thus it is important to use this resource wisely. Prior knowledge in long-term memory can aid in efficient encoding of information by allowing for the prioritization of novel stimuli over familiar ones. Here we used a full-report procedure in a visual working memory pa...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-12-17 00:00:00
abstract：:In the last forty years therapy outcomes for depression have remained the same with approximately 50% of patients responding to treatments. Advances are urgently required. We hypothesised that a recent treatment, metacognitive therapy (MCT), might be more effective, by targeting mental control processes that directly ...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
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更新日期：2020-05-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17264
更新日期：2015-11-25 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11297
更新日期：2015-06-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22444-0
更新日期：2018-03-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-02-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10269
更新日期：2015-06-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08356-5
更新日期：2017-08-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34096
更新日期：2016-09-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22074
更新日期：2016-02-26 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03797
更新日期：2014-01-21 00:00:00
abstract：:Cell-based assays are growing in importance for screening drugs and investigating their mechanisms of action. Most of the assays use so-called "normal" cell strain because it is difficult to produce cell lines in which the disease conditions are reproduced. In this study, we used a cell-resealing technique, which reve...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15443-0
更新日期：2017-11-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22832
更新日期：2016-03-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41231-z
更新日期：2019-03-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-11-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-01-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57855-5
更新日期：2020-01-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-08-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2015-10-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-10-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27102
更新日期：2016-06-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80757-5
更新日期：2021-01-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30042-3
更新日期：2018-08-03 00:00:00
abstract：:Long-term high-fat diet (HFD) consumption leads to not only obese-insulin resistance, but also impaired left ventricular (LV) function. Vagus nerve stimulation (VNS) has been shown to exert cardioprotection. However, its effects on the heart and metabolic parameters under obese-insulin resistant condition is not known...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19749
更新日期：2016-02-01 00:00:00
abstract：:The objective was to measure the association between badger culling and bovine tuberculosis (TB) incidents in cattle herds in three areas of England between 2013-2017 (Gloucestershire and Somerset) and 2015-2017 (Dorset). Farming industry-selected licensed culling areas were matched to comparison areas. A TB incident ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49957-6
更新日期：2019-10-11 00:00:00
abstract：:The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indonesia, almost all str...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24406-y
更新日期：2018-04-17 00:00:00
