Abstract：
:Accumulation of unfolded proteins within the endoplasmic reticulum (ER) of eukaryotic cells leads to an unfolded protein response (UPR) that either restores homeostasis or commits the cells to apoptosis. Tools traditionally used to study the UPR are proapoptotic and thus confound analysis of long-term cellular responses to ER stress. Here, we describe an ER-localized HaloTag (ERHT) protein that can be conditionally destabilized using a small-molecule hydrophobic tag (HyT36). Treatment of ERHT-expressing cells with HyT36 induces acute, resolvable ER stress that results in transient UPR activation without induction of apoptosis. Transcriptome analysis of late-stage responses to this UPR stimulus reveals a link between UPR activity and estrogen signaling.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Raina K,Noblin DJ,Serebrenik YV,Adams A,Zhao C,Crews CMdoi
10.1038/nchembio.1638subject
Has Abstractpub_date
2014-11-01 00:00:00pages
957-62issue
11eissn
1552-4450issn
1552-4469pii
nchembio.1638journal_volume
10pub_type
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