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Tracking single molecules at work in living cells.

Abstract：

:Methods for imaging and tracking single molecules conjugated with fluorescent probes, called single-molecule tracking (SMT), are now providing researchers with the unprecedented ability to directly observe molecular behaviors and interactions in living cells. Current SMT methods are achieving almost the ultimate spatial precision and time resolution for tracking single molecules, determined by the currently available dyes. In cells, various molecular interactions and reactions occur as stochastic and probabilistic processes. SMT provides an ideal way to directly track these processes by observing individual molecules at work in living cells, leading to totally new views of the biochemical and molecular processes used by cells whether in signal transduction, gene regulation or formation and disintegration of macromolecular complexes. Here we review SMT methods, summarize the recent results obtained by SMT, including related superresolution microscopy data, and describe the special concerns when SMT applications are shifted from the in vitro paradigms to living cells.

journal_name

Nat Chem Biol

journal_title

Nature chemical biology

authors

Kusumi A,Tsunoyama TA,Hirosawa KM,Kasai RS,Fujiwara TK

doi

10.1038/nchembio.1558

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

524-32

issue

7

eissn

1552-4450

issn

1552-4469

pii

nchembio.1558

journal_volume

10

pub_type

杂志文章,评审

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