Abstract：
:Lysine riboswitches are bacterial RNA structures that sense the concentration of lysine and regulate the expression of lysine biosynthesis and transport genes. Members of this riboswitch class are found in the 5' untranslated region of messenger RNAs, where they form highly selective receptors for lysine. Lysine binding to the receptor stabilizes an mRNA tertiary structure that, in most cases, causes transcription termination before the adjacent open reading frame can be expressed. A lysine riboswitch conceivably could be targeted for antibacterial therapy by designing new compounds that bind the riboswitch and suppress lysine biosynthesis and transport genes. As a test of this strategy, we have identified several lysine analogs that bind to riboswitches in vitro and inhibit Bacillus subtilis growth, probably through a mechanism of riboswitch-mediated repression of lysine biosynthesis. These results indicate that riboswitches could serve as new classes of antibacterial drug targets.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Blount KF,Wang JX,Lim J,Sudarsan N,Breaker RRdoi
10.1038/nchembio842subject
Has Abstractpub_date
2007-01-01 00:00:00pages
44-9issue
1eissn
1552-4450issn
1552-4469pii
nchembio842journal_volume
3pub_type
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