Dynamic membrane topology in an unassembled membrane protein.

Abstract:

:Helical membrane proteins are typically assumed to attain stable transmembrane topologies immediately upon co-translational membrane insertion. Here we show that unassembled monomers of the small multidrug resistance (SMR) family exist in a dynamic equilibrium where the N-terminal transmembrane helix flips in and out of the membrane, with rates that depend on dimerization and the polypeptide sequence. Thus, membrane topology can display rapid dynamics in vivo and can be regulated by post-translational assembly.

journal_name

Nat Chem Biol

journal_title

Nature chemical biology

authors

Seurig M,Ek M,von Heijne G,Fluman N

doi

10.1038/s41589-019-0356-9

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

945-948

issue

10

eissn

1552-4450

issn

1552-4469

pii

10.1038/s41589-019-0356-9

journal_volume

15

pub_type

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