Abstract:
:Chemical biology is an interdisciplinary field that is undergoing rapid expansion around the globe. Recently, the Japanese Society for Chemical Biology sponsored its inaugural scientific meeting to discuss research at the interface of chemistry and biology.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Kikuchi K,Kakeya Hdoi
10.1038/nchembio0806-392subject
Has Abstractpub_date
2006-08-01 00:00:00pages
392-4issue
8eissn
1552-4450issn
1552-4469pii
nchembio0806-392journal_volume
2pub_type
abstract::The catalytic activity of many protein kinases is controlled by conformational changes of a conserved Asp-Phe-Gly (DFG) motif. We used an infrared probe to track the DFG motif of the mitotic kinase Aurora A (AurA) and found that allosteric activation by the spindle-associated protein Tpx2 involves an equilibrium shift...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2296
更新日期:2017-04-01 00:00:00
abstract::CRISPR-Cas9 has emerged as a powerful technology that enables ready modification of the mammalian genome. The ability to modulate Cas9 activity can reduce off-target cleavage and facilitate precise genome engineering. Here we report the development of a Cas9 variant whose activity can be switched on and off in human c...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2179
更新日期:2016-11-01 00:00:00
abstract::Synthetic mRNA is an attractive vehicle for gene therapies because of its transient nature and improved safety profile over DNA. However, unlike DNA, broadly applicable methods to control expression from mRNA are lacking. Here we describe a platform for small-molecule-based regulation of expression from modified RNA (...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-018-0146-9
更新日期:2018-11-01 00:00:00
abstract::ADP-ribosylation (ADPr) is a biologically and clinically important post-translational modification, but little is known about the amino acids it targets on cellular proteins. Here we present a proteomic approach for direct in vivo identification and quantification of ADPr sites on histones. We have identified 12 uniqu...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2180
更新日期:2016-12-01 00:00:00
abstract::Secondary structure refolding is a key event in biology as it modulates the conformation of many proteins in the cell, generating functional or aberrant states. The crystal structures of mannosyltransferase PimA reveal an exceptional flexibility of the protein along the catalytic cycle, including β-strand-to-α-helix a...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1694
更新日期:2015-01-01 00:00:00
abstract::Tyrian purple, mainly composed of 6,6'-dibromoindigo (6BrIG), is an ancient dye extracted from sea snails and was recently demonstrated as a biocompatible semiconductor material. However, its synthesis remains limited due to uncharacterized biosynthetic pathways and the difficulty of regiospecific bromination. Here, w...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-020-00684-4
更新日期:2021-01-01 00:00:00
abstract::The chemical scaffolds from which screening libraries are built have strong influence on the libraries' utility for screening campaigns. Here we present analysis of the scaffold composition of several types of commercially available screening collections and compare those compositions to those of drugs and drug candid...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio0807-442
更新日期:2007-08-01 00:00:00
abstract::Intracellular Ca(2+) regulates numerous proteins and cellular functions and can vary substantially over submicron and submillisecond scales, so precisely localized fast detection is desirable. We have created a approximately 1-kDa biarsenical Ca(2+) indicator, called Calcium Green FlAsH (CaGF, 1), to probe [Ca(2+)] su...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2007.4
更新日期:2007-07-01 00:00:00
abstract::The function of many biologically active molecules requires the presence of carbon-nitrogen bonds in strategic positions. The biosynthetic pathways leading to such bonds can be bypassed through chemical synthesis to synthesize natural products more efficiently and also to generate the molecular diversity unavailable i...
journal_title:Nature chemical biology
pub_type: 杂志文章,评审
doi:10.1038/nchembio0606-284
更新日期:2006-06-01 00:00:00
abstract::Trafficking and sorting of membrane-anchored Ras GTPases are regulated by partitioning between distinct membrane domains. Here, in vitro experiments and microscopic molecular theory reveal membrane curvature as a new modulator of N-Ras lipid anchor and palmitoyl chain partitioning. Membrane curvature was essential for...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1733
更新日期:2015-03-01 00:00:00
abstract::Small ubiquitin-like modifier (SUMO) family proteins regulate target-protein functions by post-translational modification. However, a potent and selective inhibitor targeting the SUMO pathway has been lacking. Here we describe ML-792, a mechanism-based SUMO-activating enzyme (SAE) inhibitor with nanomolar potency in c...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2463
更新日期:2017-11-01 00:00:00
abstract::Cholinesterase inhibitors, the current frontline symptomatic treatment for Alzheimer's disease (AD), are associated with low efficacy and adverse effects. M1 muscarinic acetylcholine receptors (M1 mAChRs) represent a potential alternate therapeutic target; however, drug discovery programs focused on this G protein-cou...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-019-0453-9
更新日期:2020-03-01 00:00:00
abstract::Chemistry drives many biological interactions between the microbiota and host animals, yet it is often challenging to identify the chemicals involved. This poses a problem, as such small molecules are excellent sources of potential pharmaceuticals, pretested by nature for animal compatibility. We discovered anti-HIV c...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2537
更新日期:2018-02-01 00:00:00
abstract::The lasso peptide microcin J25 is known to hijack the siderophore receptor FhuA for initiating internalization. Here, we provide what is to our knowledge the first structural evidence on the recognition mechanism, and our biochemical data show that another closely related lasso peptide cannot interact with FhuA. Our w...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1499
更新日期:2014-05-01 00:00:00
abstract::N6-Methyladenosine (m6A) RNA modification is present in messenger RNAs (mRNA), ribosomal RNAs (rRNA), and spliceosomal RNAs (snRNA) in humans. Although mRNA m6A modifications have been extensively studied and shown to play critical roles in many cellular processes, the identity of m6A methyltransferases for rRNAs and ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-018-0184-3
更新日期:2019-01-01 00:00:00
abstract::The chemical species involved in H2S signaling remain elusive despite the profound and pleiotropic physiological effects elicited by this molecule. The dominant candidate mechanism for sulfide signaling is persulfidation of target proteins. However, the relatively poor reactivity of H2S toward oxidized thiols, such as...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1834
更新日期:2015-07-01 00:00:00
abstract::Kinetochores act as hubs for multiple activities during cell division, including microtubule interactions and spindle checkpoint signaling. Each kinetochore can act autonomously, and activities change rapidly as proteins are recruited to, or removed from, kinetochores. Understanding this dynamic system requires tools ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2456
更新日期:2017-10-01 00:00:00
abstract::Actin filament dynamics are critical in cell motility. The structure of actin filament changes spontaneously and can also be regulated by actin-binding proteins, allowing actin to readily function in response to external stimuli. The interaction with the motor protein myosin changes the dynamic nature of actin filamen...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio763
更新日期:2006-02-01 00:00:00
abstract::The resistance of Caenorhabditis elegans to pharmacological perturbation limits its use as a screening tool for novel small bioactive molecules. One strategy to improve the hit rate of small-molecule screens is to preselect molecules that have an increased likelihood of reaching their target in the worm. To learn whic...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.380
更新日期:2010-07-01 00:00:00
abstract::Pseudouridine synthases (PUSs) are responsible for installation of pseudouridine (Ψ) modification in RNA. However, the activity and function of the PUS enzymes remain largely unexplored. Here we focus on human PUS10 and find that it co-expresses with the microprocessor (DROSHA-DGCR8 complex). Depletion of PUS10 result...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-019-0420-5
更新日期:2020-02-01 00:00:00
abstract::Studies of gene function and molecular mechanisms in Plasmodium falciparum are hampered by difficulties in characterizing and measuring phenotypic differences between individual parasites. We screened seven parasite lines for differences in responses to 1,279 bioactive chemicals. Hundreds of compounds were active in i...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.215
更新日期:2009-10-01 00:00:00
abstract::RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-su...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2228
更新日期:2017-01-01 00:00:00
abstract:: ...
journal_title:Nature chemical biology
pub_type: 评论,杂志文章
doi:10.1038/s41589-018-0073-9
更新日期:2018-06-01 00:00:00
abstract::Ferroptosis is a form of regulated necrotic cell death controlled by glutathione peroxidase 4 (GPX4). At present, mechanisms that could predict sensitivity and/or resistance and that may be exploited to modulate ferroptosis are needed. We applied two independent approaches-a genome-wide CRISPR-based genetic screen and...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2239
更新日期:2017-01-01 00:00:00
abstract::The active site of [NiFe] hydrogenases contains a strictly conserved arginine that suspends a guanidine nitrogen atom <4.5 Å above the nickel and iron atoms. The guanidine headgroup interacts with the side chains of two conserved aspartic acid residues to complete an outer-shell canopy that has thus far proved intract...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1976
更新日期:2016-01-01 00:00:00
abstract::Methods to evolve synthetic, rather than biological, polymers could significantly expand the functional potential of polymers that emerge from in vitro evolution. Requirements for synthetic polymer evolution include (i) sequence-specific polymerization of synthetic building blocks on an amplifiable template, (ii) disp...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.280
更新日期:2010-02-01 00:00:00
abstract::Proteoglycans are complex glycoconjugates that regulate critical biological pathways in all higher organisms. Bikunin, the simplest proteoglycan, with a single glycosaminoglycan chain, is a serine protease inhibitor used to treat acute pancreatitis. Unlike nucleic acids and proteins, whose synthesis is template driven...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.673
更新日期:2011-10-09 00:00:00
abstract::Using linewidth and spinning sideband intensities of lipid hydrocarbon chain resonances in proton magic angle spinning NMR spectra, we detected the temperature-dependent phase state of naturally occurring lipids of intact influenza virus without exogenous probes. Increasingly, below 41 degrees C ordered and disordered...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.77
更新日期:2008-04-01 00:00:00
abstract::Identifying targets of antibacterial compounds remains a challenging step in the development of antibiotics. We have developed a two-pronged functional genomics approach to predict mechanism of action that uses mutant fitness data from antibiotic-treated transposon libraries containing both upregulation and inactivati...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-018-0041-4
更新日期:2018-06-01 00:00:00
abstract::It is postulated that in addition to cell density, other factors such as the dimensions and diffusional characteristics of the environment could influence quorum sensing (QS) and induction of genetic reprogramming. Modeling studies predict that QS may operate at the level of a single cell, but, owing to experimental c...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.264
更新日期:2010-01-01 00:00:00