Abstract:
:
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Deane Cdoi
10.1038/s41589-018-0073-9subject
pub_date
2018-06-01 00:00:00pages
525issue
6eissn
1552-4450issn
1552-4469pii
10.1038/s41589-018-0073-9journal_volume
14pub_type
评论,杂志文章abstract::Small molecules that specifically activate an intracellular protein of interest are highly desirable. A generally applicable strategy, however, remains elusive. Herein we describe a small molecule-triggered bioorthogonal protein decaging technique that relies on the inverse electron-demand Diels-Alder reaction for eli...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1656
更新日期:2014-12-01 00:00:00
abstract::Conditional cooperativity is a common mechanism involved in transcriptional regulation of prokaryotic type II toxin-antitoxin operons and is intricately related to bacterial persistence. It allows the toxin component of a toxin-antitoxin module to act as a co-repressor at low doses of toxin as compared to antitoxin. W...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2078
更新日期:2016-07-01 00:00:00
abstract::Proteoglycans are complex glycoconjugates that regulate critical biological pathways in all higher organisms. Bikunin, the simplest proteoglycan, with a single glycosaminoglycan chain, is a serine protease inhibitor used to treat acute pancreatitis. Unlike nucleic acids and proteins, whose synthesis is template driven...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.673
更新日期:2011-10-09 00:00:00
abstract::Methods for imaging and tracking single molecules conjugated with fluorescent probes, called single-molecule tracking (SMT), are now providing researchers with the unprecedented ability to directly observe molecular behaviors and interactions in living cells. Current SMT methods are achieving almost the ultimate spati...
journal_title:Nature chemical biology
pub_type: 杂志文章,评审
doi:10.1038/nchembio.1558
更新日期:2014-07-01 00:00:00
abstract::CRISPR-Cas9 has emerged as a powerful technology that enables ready modification of the mammalian genome. The ability to modulate Cas9 activity can reduce off-target cleavage and facilitate precise genome engineering. Here we report the development of a Cas9 variant whose activity can be switched on and off in human c...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2179
更新日期:2016-11-01 00:00:00
abstract::Nontranslational functions of vertebrate aminoacyl tRNA synthetases (aaRSs), which catalyze the production of aminoacyl-tRNAs for protein synthesis, have recently been discovered. Although these new functions were thought to be 'moonlighting activities', many are as critical for cellular homeostasis as their activity ...
journal_title:Nature chemical biology
pub_type: 杂志文章,评审
doi:10.1038/nchembio.1158
更新日期:2013-03-01 00:00:00
abstract::Bacteria are thought to cope with fluctuating environmental solute concentrations primarily by adjusting the osmolality of their cytoplasm. To obtain insights into the underlying metabolic adaptations, we analyzed the global metabolic response of Escherichia coli to sustained hyperosmotic stress using nontargeted mass...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1437
更新日期:2014-04-01 00:00:00
abstract::ADP-ribosylation is a post-translational modification that is known to be involved in cellular homeostasis and stress but has been challenging to analyze biochemically. To facilitate the detection of ADP-ribosylated proteins, we show that an alkyne-adenosine analog, N6-propargyl adenosine (N6pA), is metabolically inco...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2280
更新日期:2017-03-01 00:00:00
abstract::We describe a new technology for recruiting specific proteins to RNA through selective recognition of heteroduplexes formed with chemically modified antisense oligonucleotides (ASOs). Typically, ASOs function by hybridizing to their RNA targets and blocking the binding of single-stranded RNA-binding proteins. Unexpect...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.939
更新日期:2012-04-15 00:00:00
abstract::Beyond established roles in collagen biosynthesis, hypoxic signaling and fatty acid metabolism, recent reports have now revealed roles for human 2-oxoglutarate-dependent oxygenases in histone and nucleic acid demethylation and in signaling protein hydroxylation. The emerging role of these oxygenases in enabling a mult...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio0308-152
更新日期:2008-03-01 00:00:00
abstract::The eukaryotic 26S proteasome controls cellular processes by degrading specific regulatory proteins. Most proteins are targeted for degradation by a signal or degron that consists of two parts: a proteasome-binding tag, typically covalently attached polyubiquitin chains, and an unstructured region that serves as the i...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.521
更新日期:2011-03-01 00:00:00
abstract::Serine is both a proteinogenic amino acid and the source of one-carbon units essential for de novo purine and deoxythymidine synthesis. In the canonical pathway of glucose-derived serine synthesis, Homo sapiens phosphoglycerate dehydrogenase (PHGDH) catalyzes the first, rate-limiting step. Genetic loss of PHGDH is tox...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2070
更新日期:2016-06-01 00:00:00
abstract::The sea lamprey is an ancient, parasitic fish that invaded the Great Lakes a century ago, where it triggered the collapse of many fisheries. Like many fishes, this species relies on chemical cues to mediate key aspects of its life, including migration and reproduction. Here we report the discovery of a multicomponent ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio739
更新日期:2005-11-01 00:00:00
abstract::Methods to evolve synthetic, rather than biological, polymers could significantly expand the functional potential of polymers that emerge from in vitro evolution. Requirements for synthetic polymer evolution include (i) sequence-specific polymerization of synthetic building blocks on an amplifiable template, (ii) disp...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.280
更新日期:2010-02-01 00:00:00
abstract::Nitric oxide (NO) signaling through the formation of cGMP is well established; however, there seems to be an increasing role for cGMP-independent NO signaling. Although key molecular details remain unanswered, S-nitrosation represents an example of cGMP-independent NO signaling. This modification has garnered recent a...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio720
更新日期:2005-08-01 00:00:00
abstract::The P1B-ATPases, which couple cation transport across membranes to ATP hydrolysis, are central to metal homeostasis in all organisms. An important feature of P1B-ATPases is the presence of soluble metal binding domains (MBDs) that regulate transport activity. Only one type of MBD has been characterized extensively, bu...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1863
更新日期:2015-09-01 00:00:00
abstract::Oxidase and oxygenase enzymes allow the use of relatively unreactive O2 in biochemical reactions. Many of the mechanistic strategies used in nature for this key reaction are represented within the 2-histidine-1-carboxylate facial triad family of non-heme Fe(II)-containing enzymes. The open face of the metal coordinati...
journal_title:Nature chemical biology
pub_type: 杂志文章,评审
doi:10.1038/nchembio.71
更新日期:2008-03-01 00:00:00
abstract::Signal transduction, regulatory processes and pharmaceutical responses are highly dependent upon ligand residence times. Gaining insight into how physical factors influence residence times (1/k(off)) should enhance our ability to manipulate biological interactions. We report experiments that yield structural insight i...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.769
更新日期:2012-01-15 00:00:00
abstract::The N-end rule pathway senses the N-terminal destabilizing residues of degradation substrates for the ubiquitin-proteasome system, whose integrity shields against various human syndromes including cancer and cardiovascular diseases. GID4, a subunit of the ubiquitin ligase GID complex, has been recently identified as t...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-018-0036-1
更新日期:2018-05-01 00:00:00
abstract::The actin cytoskeleton supports diverse cellular processes such as endocytosis, oriented growth, adhesion and migration. The dynamic nature of the cytoskeleton, however, has made it difficult to define the roles of the many accessory molecules that modulate actin organization, especially the multifunctional adapter pr...
journal_title:Nature chemical biology
pub_type: 信件
doi:10.1038/nchembio755
更新日期:2006-01-01 00:00:00
abstract::Cholinesterase inhibitors, the current frontline symptomatic treatment for Alzheimer's disease (AD), are associated with low efficacy and adverse effects. M1 muscarinic acetylcholine receptors (M1 mAChRs) represent a potential alternate therapeutic target; however, drug discovery programs focused on this G protein-cou...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-019-0453-9
更新日期:2020-03-01 00:00:00
abstract::Synthetic mRNA is an attractive vehicle for gene therapies because of its transient nature and improved safety profile over DNA. However, unlike DNA, broadly applicable methods to control expression from mRNA are lacking. Here we describe a platform for small-molecule-based regulation of expression from modified RNA (...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-018-0146-9
更新日期:2018-11-01 00:00:00
abstract:: ...
journal_title:Nature chemical biology
pub_type: 评论,杂志文章
doi:10.1038/s41589-019-0230-9
更新日期:2019-03-01 00:00:00
abstract::Natural enzymes have evolved to perform their cellular functions under complex selective pressures, which often require their catalytic activities to be regulated by other proteins. We contrasted a natural enzyme, LovD, which acts on a protein-bound (LovF) acyl substrate, with a laboratory-generated variant that was t...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1503
更新日期:2014-06-01 00:00:00
abstract::A required course in communication at the graduate school level may help cure the disorder of poor scientific writing. ...
journal_title:Nature chemical biology
pub_type: 社论
doi:10.1038/nchembio0906-443
更新日期:2006-09-01 00:00:00
abstract::Combination antiretroviral therapy has transformed HIV-1 infection, once a fatal illness, into a manageable chronic condition. Drug resistance, severe side effects and treatment noncompliance bring challenges to combination antiretroviral therapy implementation in clinical settings and indicate the need for additional...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-020-0496-y
更新日期:2020-05-01 00:00:00
abstract::We describe Scaffold Hunter, a highly interactive computer-based tool for navigation in chemical space that fosters intuitive recognition of complex structural relationships associated with bioactivity. The program reads compound structures and bioactivity data, generates compound scaffolds, correlates them in a hiera...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.187
更新日期:2009-08-01 00:00:00
abstract::Lysine riboswitches are bacterial RNA structures that sense the concentration of lysine and regulate the expression of lysine biosynthesis and transport genes. Members of this riboswitch class are found in the 5' untranslated region of messenger RNAs, where they form highly selective receptors for lysine. Lysine bindi...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio842
更新日期:2007-01-01 00:00:00
abstract::Only 25% of bacterial membrane transporters have functional annotation owing to the difficulty of experimental study and of accurate prediction of their function. Here we report a sequence-independent method for high-throughput mining of novel transporters. The method is based on ligand-responsive biosensor systems th...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2189
更新日期:2016-12-01 00:00:00
abstract::The rise of antibiotic resistance demands the acceleration of molecular diversification strategies to inspire new chemical entities for antibiotic medicines. We report here on the large-scale engineering of ribosomally synthesized and post-translationally modified antimicrobial peptides carrying the ring-forming amino...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-019-0250-5
更新日期:2019-05-01 00:00:00