Abstract：
:Conditional cooperativity is a common mechanism involved in transcriptional regulation of prokaryotic type II toxin-antitoxin operons and is intricately related to bacterial persistence. It allows the toxin component of a toxin-antitoxin module to act as a co-repressor at low doses of toxin as compared to antitoxin. When toxin level exceeds a certain threshold, however, the toxin becomes a de-repressor. Most antitoxins contain an intrinsically disordered region (IDR) that typically is involved in toxin neutralization and repressor complex formation. To address how the antitoxin IDR is involved in transcription regulation, we studied the phd-doc operon from bacteriophage P1. We provide evidence that the IDR of Phd provides an entropic barrier precluding full operon repression in the absence of Doc. Binding of Doc results in a cooperativity switch and consequent strong operon repression, enabling context-specific modulation of the regulatory process. Variations of this theme are likely to be a common mechanism in the autoregulation of bacterial operons that involve intrinsically disordered regions.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Garcia-Pino A,De Gieter S,Talavera A,De Greve H,Efremov RG,Loris Rdoi
10.1038/nchembio.2078subject
Has Abstractpub_date
2016-07-01 00:00:00pages
490-6issue
7eissn
1552-4450issn
1552-4469pii
nchembio.2078journal_volume
12pub_type
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