Abstract:
:Nature is a diverse and rich source of bioactive pathways or novel building blocks for synthetic biology. In this Perspective, we describe the emerging research field in which metagenomes are functionally interrogated using synthetic biology. This approach substantially expands the set of identified biological activities and building blocks. In reviewing this field, we find that its potential for new biological discovery is dramatically increasing. Functional metagenomic mining using genetic circuits has led to the discovery of novel bioactivity such as amidases, NF-κB modulators, naphthalene degrading enzymes, cellulases, lipases and transporters. Using these genetic circuits as a template, improvements are made by designing biosensors, such as in vitro-evolved riboswitches and computationally redesigned transcription factors. Thus, powered by the rapidly expanding repertoire of biosensors and streamlined processes for automated genetic circuit design, a greater variety of complex selection circuits can be built, with resulting impacts on drug discovery and industrial biotechnology.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
van der Helm E,Genee HJ,Sommer MOAdoi
10.1038/s41589-018-0100-xsubject
Has Abstractpub_date
2018-08-01 00:00:00pages
752-759issue
8eissn
1552-4450issn
1552-4469pii
10.1038/s41589-018-0100-xjournal_volume
14pub_type
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