The evolving interface between synthetic biology and functional metagenomics.

Abstract:

:Nature is a diverse and rich source of bioactive pathways or novel building blocks for synthetic biology. In this Perspective, we describe the emerging research field in which metagenomes are functionally interrogated using synthetic biology. This approach substantially expands the set of identified biological activities and building blocks. In reviewing this field, we find that its potential for new biological discovery is dramatically increasing. Functional metagenomic mining using genetic circuits has led to the discovery of novel bioactivity such as amidases, NF-κB modulators, naphthalene degrading enzymes, cellulases, lipases and transporters. Using these genetic circuits as a template, improvements are made by designing biosensors, such as in vitro-evolved riboswitches and computationally redesigned transcription factors. Thus, powered by the rapidly expanding repertoire of biosensors and streamlined processes for automated genetic circuit design, a greater variety of complex selection circuits can be built, with resulting impacts on drug discovery and industrial biotechnology.

journal_name

Nat Chem Biol

journal_title

Nature chemical biology

authors

van der Helm E,Genee HJ,Sommer MOA

doi

10.1038/s41589-018-0100-x

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

752-759

issue

8

eissn

1552-4450

issn

1552-4469

pii

10.1038/s41589-018-0100-x

journal_volume

14

pub_type

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