Diels-Alder reaction-triggered bioorthogonal protein decaging in living cells.

Abstract:

:Small molecules that specifically activate an intracellular protein of interest are highly desirable. A generally applicable strategy, however, remains elusive. Herein we describe a small molecule-triggered bioorthogonal protein decaging technique that relies on the inverse electron-demand Diels-Alder reaction for eliminating a chemically caged protein side chain within living cells. This method permits the efficient activation of a given protein (for example, an enzyme) in its native cellular context within minutes.

journal_name

Nat Chem Biol

journal_title

Nature chemical biology

authors

Li J,Jia S,Chen PR

doi

10.1038/nchembio.1656

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

1003-5

issue

12

eissn

1552-4450

issn

1552-4469

pii

nchembio.1656

journal_volume

10

pub_type

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