Abstract:
:Small molecules that specifically activate an intracellular protein of interest are highly desirable. A generally applicable strategy, however, remains elusive. Herein we describe a small molecule-triggered bioorthogonal protein decaging technique that relies on the inverse electron-demand Diels-Alder reaction for eliminating a chemically caged protein side chain within living cells. This method permits the efficient activation of a given protein (for example, an enzyme) in its native cellular context within minutes.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Li J,Jia S,Chen PRdoi
10.1038/nchembio.1656subject
Has Abstractpub_date
2014-12-01 00:00:00pages
1003-5issue
12eissn
1552-4450issn
1552-4469pii
nchembio.1656journal_volume
10pub_type
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