Abstract：
:We report that simple glycoside donors can drastically shift the equilibria of glycosyltransferase-catalyzed reactions, transforming NDP-sugar formation from an endothermic to an exothermic process. To demonstrate the utility of this thermodynamic adaptability, we highlight the glycosyltransferase-catalyzed synthesis of 22 sugar nucleotides from simple aromatic sugar donors, as well as the corresponding in situ formation of sugar nucleotides as a driving force in the context of glycosyltransferase-catalyzed reactions for small-molecule glycodiversification. These simple aromatic donors also enabled a general colorimetric assay for glycosyltransfer, applicable to drug discovery, protein engineering and other fundamental sugar nucleotide-dependent investigations. This study directly challenges the general notion that NDP-sugars are 'high-energy' sugar donors when taken out of their traditional biological context.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Gantt RW,Peltier-Pain P,Cournoyer WJ,Thorson JSdoi
10.1038/nchembio.638subject
Has Abstractpub_date
2011-08-21 00:00:00pages
685-91issue
10eissn
1552-4450issn
1552-4469pii
nchembio.638journal_volume
7pub_type
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