Abstract：
:Transcription factors have diverse roles during embryonic development, combinatorially controlling cellular states in a spatially and temporally defined manner. Resolving the dynamic transcriptional profiles that underlie these patterning processes is essential for understanding embryogenesis at the molecular level. Here we show how temporal, tissue-specific changes in embryonic transcription factor function can be discerned by integrating caged morpholino oligonucleotides with photoactivatable fluorophores, fluorescence-activated cell sorting and microarray technologies. As a proof of principle, we have dynamically profiled No tail a (Ntla)-dependent genes at different stages of axial mesoderm development in zebrafish, discovering discrete sets of transcripts that are coincident with either notochord cell fate commitment or differentiation. Our studies reveal new regulators of notochord development and the sequential activation of distinct transcriptomes within a cell lineage by a single transcriptional factor and demonstrate how optically controlled chemical tools can dissect developmental processes with spatiotemporal precision.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Shestopalov IA,Pitt CL,Chen JKdoi
10.1038/nchembio.772subject
Has Abstractpub_date
2012-01-29 00:00:00pages
270-6issue
3eissn
1552-4450issn
1552-4469pii
nchembio.772journal_volume
8pub_type
杂志文章abstract：:Changes in the cellular environment modulate protein energy landscapes to drive important biology, with consequences for signaling, allostery and other vital processes. The effects of ubiquitination are particularly important because of their potential influence on degradation by the 26S proteasome. Moreover, proteaso...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-020-0556-3
更新日期：2020-08-01 00:00:00
abstract：:Methylation of nucleotides in ribosomal RNAs (rRNAs) is a ubiquitous feature that occurs in all living organisms. Identification of all enzymes responsible for rRNA methylation, as well as mapping of all modified rRNA residues, is now complete for a number of model species, such as Escherichia coli and Saccharomyces c...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio.2569
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journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio876
更新日期：2007-05-01 00:00:00
abstract：:ADP-ribosylation is a post-translational modification that is known to be involved in cellular homeostasis and stress but has been challenging to analyze biochemically. To facilitate the detection of ADP-ribosylated proteins, we show that an alkyne-adenosine analog, N6-propargyl adenosine (N6pA), is metabolically inco...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2280
更新日期：2017-03-01 00:00:00
abstract：:We describe Scaffold Hunter, a highly interactive computer-based tool for navigation in chemical space that fosters intuitive recognition of complex structural relationships associated with bioactivity. The program reads compound structures and bioactivity data, generates compound scaffolds, correlates them in a hiera...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.187
更新日期：2009-08-01 00:00:00
abstract：:Methods for imaging and tracking single molecules conjugated with fluorescent probes, called single-molecule tracking (SMT), are now providing researchers with the unprecedented ability to directly observe molecular behaviors and interactions in living cells. Current SMT methods are achieving almost the ultimate spati...
journal_title：Nature chemical biology
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journal_title：Nature chemical biology
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journal_title：Nature chemical biology
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journal_title：Nature chemical biology
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abstract：: ...
journal_title：Nature chemical biology
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更新日期：2018-06-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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journal_title：Nature chemical biology
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更新日期：2007-02-01 00:00:00
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journal_title：Nature chemical biology
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更新日期：2020-02-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章,评审
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更新日期：2013-03-01 00:00:00
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journal_title：Nature chemical biology
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doi：10.1038/nchembio.1357
更新日期：2013-11-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
abstract：:Hundreds of cellular proteins require iron cofactors for activity, and cells express systems for their assembly and distribution. Molecular details of the cytosolic iron pool used for iron cofactors are lacking, but iron chaperones of the poly(rC)-binding protein (PCBP) family play a key role in ferrous ion distributi...
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abstract：:Targeted drugs are effective when they directly inhibit strong disease drivers, but only a small fraction of diseases feature defined actionable drivers. Alternatively, network-based approaches can uncover new therapeutic opportunities. Applying an integrated phenotypic screening, chemical and phosphoproteomics strate...
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journal_title：Nature chemical biology
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更新日期：2009-07-01 00:00:00
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journal_title：Nature chemical biology
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更新日期：2018-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Nature chemical biology
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更新日期：2011-03-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2016-12-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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journal_title：Nature chemical biology
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journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.551
更新日期：2011-05-01 00:00:00
