Abstract：
:Pseudouridine synthases (PUSs) are responsible for installation of pseudouridine (Ψ) modification in RNA. However, the activity and function of the PUS enzymes remain largely unexplored. Here we focus on human PUS10 and find that it co-expresses with the microprocessor (DROSHA-DGCR8 complex). Depletion of PUS10 results in a marked reduction of the expression level of a large number of mature miRNAs and concomitant accumulation of unprocessed primary microRNAs (pri-miRNAs) in multiple human cells. Mechanistically, PUS10 directly binds to pri-miRNAs and interacts with the microprocessor to promote miRNA biogenesis. Unexpectedly, this process is independent of the catalytic activity of PUS10. Additionally, we develop a sequencing method to profile Ψ in the tRNAome and report PUS10-dependent Ψ sites in tRNA. Collectively, our findings reveal differential functions of PUS10 in nuclear miRNA processing and in cytoplasmic tRNA pseudouridylation.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Song J,Zhuang Y,Zhu C,Meng H,Lu B,Xie B,Peng J,Li M,Yi Cdoi
10.1038/s41589-019-0420-5subject
Has Abstractpub_date
2020-02-01 00:00:00pages
160-169issue
2eissn
1552-4450issn
1552-4469pii
10.1038/s41589-019-0420-5journal_volume
16pub_type
杂志文章abstract：:Clinically significant antibiotic resistance has evolved against virtually every antibiotic deployed. Yet the development of new classes of antibiotics has lagged far behind our growing need for such drugs. Rather than focusing on therapeutics that target in vitro viability, much like conventional antibiotics, an alte...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
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更新日期：2007-09-01 00:00:00
abstract：:Thioredoxin (Trx) is one of the two major thiol antioxidants, playing essential roles in redox homeostasis and signaling. Despite its importance, there is a lack of methods for monitoring Trx redox dynamics in live cells, hindering a better understanding of physiological and pathological roles of the Trx redox system....
journal_title：Nature chemical biology
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abstract：:Targeted protein degradation via small-molecule modulation of cereblon offers vast potential for the development of new therapeutics. Cereblon-binding therapeutics carry the safety risks of thalidomide, which caused an epidemic of severe birth defects characterized by forelimb shortening or phocomelia. Here we show th...
journal_title：Nature chemical biology
pub_type： 杂志文章
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abstract：:Regulation of gene expression in response to the changing environment is critical for cell survival. For instance, binding of macrolide antibiotics to the ribosome promotes translation arrest at the leader open reading frames ermCL and ermBL, which is necessary for inducing the antibiotic resistance genes ermC and erm...
journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2016-03-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章,评审
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更新日期：2007-10-01 00:00:00
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更新日期：2014-07-01 00:00:00
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pub_type： 信件
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更新日期：2006-01-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2016-05-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章,评审
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更新日期：2009-05-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
abstract：:We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revea...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2068
更新日期：2016-06-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 评论,新闻
doi：10.1038/nchembio0705-69
更新日期：2005-07-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
abstract：: ...
journal_title：Nature chemical biology
pub_type： 评论,杂志文章
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更新日期：2019-01-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2347
更新日期：2017-06-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1623
更新日期：2014-10-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio0308-152
更新日期：2008-03-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
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更新日期：2011-05-01 00:00:00
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journal_title：Nature chemical biology
pub_type：
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更新日期：2006-02-01 00:00:00
abstract：:Targeted drugs are effective when they directly inhibit strong disease drivers, but only a small fraction of diseases feature defined actionable drivers. Alternatively, network-based approaches can uncover new therapeutic opportunities. Applying an integrated phenotypic screening, chemical and phosphoproteomics strate...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2489
更新日期：2017-12-01 00:00:00
abstract：:This Commentary clarifies the meaning of the funnel diagram, which has been widely cited in papers on protein folding. To aid in the analysis of the funnel diagram, this Commentary reviews historical approaches to understanding the mechanism of protein folding. The primary role of free energy in protein folding is dis...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.565
更新日期：2011-06-17 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.543
更新日期：2011-05-01 00:00:00
abstract：:Nonribosomal peptide antibiotics, including polymyxin, vancomycin, and teixobactin, most of which contain D-amino acids, are highly effective against multidrug-resistant bacteria. However, overusing antibiotics while ignoring the risk of resistance arising has inexorably led to widespread emergence of resistant bacter...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-018-0009-4
更新日期：2018-04-01 00:00:00
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journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio842
更新日期：2007-01-01 00:00:00
abstract：:Secondary structure refolding is a key event in biology as it modulates the conformation of many proteins in the cell, generating functional or aberrant states. The crystal structures of mannosyltransferase PimA reveal an exceptional flexibility of the protein along the catalytic cycle, including β-strand-to-α-helix a...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1694
更新日期：2015-01-01 00:00:00
