Abstract:
:We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Yumerefendi H,Lerner AM,Zimmerman SP,Hahn K,Bear JE,Strahl BD,Kuhlman Bdoi
10.1038/nchembio.2068subject
Has Abstractpub_date
2016-06-01 00:00:00pages
399-401issue
6eissn
1552-4450issn
1552-4469pii
nchembio.2068journal_volume
12pub_type
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abstract:: ...
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