Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.

Abstract:

:We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.

journal_name

Nat Chem Biol

journal_title

Nature chemical biology

authors

Yumerefendi H,Lerner AM,Zimmerman SP,Hahn K,Bear JE,Strahl BD,Kuhlman B

doi

10.1038/nchembio.2068

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

399-401

issue

6

eissn

1552-4450

issn

1552-4469

pii

nchembio.2068

journal_volume

12

pub_type

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