Abstract：
:The eukaryotic 26S proteasome controls cellular processes by degrading specific regulatory proteins. Most proteins are targeted for degradation by a signal or degron that consists of two parts: a proteasome-binding tag, typically covalently attached polyubiquitin chains, and an unstructured region that serves as the initiation region for proteasomal proteolysis. Here we have characterized how the arrangement of the two degron parts in a protein affects degradation. We found that a substrate is degraded efficiently only when its initiation region is of a certain minimal length and is appropriately separated in space from the proteasome-binding tag. Regions that are located too close or too far from the proteasome-binding tag cannot access the proteasome and induce degradation. These spacing requirements are different for a polyubiquitin chain and a ubiquitin-like domain. Thus, the arrangement and location of the proteasome initiation region affect a protein's fate and are important in selecting proteins for proteasome-mediated degradation.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Inobe T,Fishbain S,Prakash S,Matouschek Adoi
10.1038/nchembio.521subject
Has Abstractpub_date
2011-03-01 00:00:00pages
161-7issue
3eissn
1552-4450issn
1552-4469pii
nchembio.521journal_volume
7pub_type
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