Abstract:
:Serine hydrolases play diverse roles in regulating host-pathogen interactions in a number of organisms, yet few have been characterized in the human pathogen Staphylococcus aureus. Here we describe a chemical proteomic screen that identified ten previously uncharacterized S. aureus serine hydrolases that mostly lack human homologs. We termed these enzymes fluorophosphonate-binding hydrolases (FphA-J). One hydrolase, FphB, can process short fatty acid esters, exhibits increased activity in response to host cell factors, is located predominantly on the bacterial cell surface in a subset of cells, and is concentrated in the division septum. Genetic disruption of fphB confirmed that the enzyme is dispensable for bacterial growth in culture but crucial for establishing infection in distinct sites in vivo. A selective small molecule inhibitor of FphB effectively reduced infectivity in vivo, suggesting that it may be a viable therapeutic target for the treatment or management of Staphylococcus infections.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Lentz CS,Sheldon JR,Crawford LA,Cooper R,Garland M,Amieva MR,Weerapana E,Skaar EP,Bogyo Mdoi
10.1038/s41589-018-0060-1subject
Has Abstractpub_date
2018-06-01 00:00:00pages
609-617issue
6eissn
1552-4450issn
1552-4469pii
10.1038/s41589-018-0060-1journal_volume
14pub_type
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