Abstract:
:More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase (Ca-NAT) has remained mysterious. Here, we use activity-based protein profiling to identify the poorly characterized serine hydrolase PLA2G4E as a mouse brain Ca-NAT and show that this enzyme generates NAPEs and NAEs in mammalian cells.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Ogura Y,Parsons WH,Kamat SS,Cravatt BFdoi
10.1038/nchembio.2127subject
Has Abstractpub_date
2016-09-01 00:00:00pages
669-71issue
9eissn
1552-4450issn
1552-4469pii
nchembio.2127journal_volume
12pub_type
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