Abstract：
:Cell polarity is the asymmetric compartmentalization of cellular components. An opposing gradient of partitioning-defective protein kinases, atypical protein kinase C (aPKC) and PAR-1, at the cell cortex guides diverse asymmetries in the structure of metazoan cells, but the mechanism underlying their spatial patterning remains poorly understood. Here, we show in Caenorhabditis elegans zygotes that the cortical PAR-1 gradient is patterned as a consequence of dual mechanisms: stabilization of cortical dynamics and protection from aPKC-mediated cortical exclusion. Dual control of cortical PAR-1 depends on a physical interaction with the PRBH-domain protein PAR-2. Using a reconstitution approach in heterologous cells, we demonstrate that PAR-1, PAR-2, and polarized Cdc42-PAR-6-aPKC comprise the minimal network sufficient for the establishment of an opposing cortical gradient. Our findings delineate the mechanism governing cortical polarity, in which a circuit consisting of aPKC and the PRBH-domain protein ensures the local recruitment of PAR-1 to a well-defined cortical compartment.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Ramanujam R,Han Z,Zhang Z,Kanchanawong P,Motegi Fdoi
10.1038/s41589-018-0117-1subject
Has Abstractpub_date
2018-10-01 00:00:00pages
917-927issue
10eissn
1552-4450issn
1552-4469pii
10.1038/s41589-018-0117-1journal_volume
14pub_type
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