Abstract:
:The lasso peptide microcin J25 is known to hijack the siderophore receptor FhuA for initiating internalization. Here, we provide what is to our knowledge the first structural evidence on the recognition mechanism, and our biochemical data show that another closely related lasso peptide cannot interact with FhuA. Our work provides an explanation on the narrow activity spectrum of lasso peptides and opens the path to the development of new antibacterials.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Mathavan I,Zirah S,Mehmood S,Choudhury HG,Goulard C,Li Y,Robinson CV,Rebuffat S,Beis Kdoi
10.1038/nchembio.1499subject
Has Abstractpub_date
2014-05-01 00:00:00pages
340-2issue
5eissn
1552-4450issn
1552-4469pii
nchembio.1499journal_volume
10pub_type
杂志文章abstract::RNA capping and decapping are thought to be distinctive features of eukaryotes. The redox cofactor NAD was recently discovered to be attached to small regulatory RNAs in bacteria in a cap-like manner, and Nudix hydrolase NudC was found to act as a NAD-decapping enzyme in vitro and in vivo. Here, crystal structures of ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2132
更新日期:2016-09-01 00:00:00
abstract::We document a new dimension of surface recognition in which communication is controlled through the collective behavior of lipids. Membrane cholesterol induces a tilt in glycolipid receptor headgroup, resulting in loss of access for ligand binding. This property appears to organize erythrocyte blood group presentation...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.551
更新日期:2011-05-01 00:00:00
abstract::Mitochondrial electron transport drives ATP synthesis but also generates reactive oxygen species, which are both cellular signals and damaging oxidants. Superoxide production by respiratory complex III is implicated in diverse signaling events and pathologies, but its role remains controversial. Using high-throughput ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1910
更新日期:2015-11-01 00:00:00
abstract::Molecular dynamics are essential for protein function. In some cases these dynamics involve the interconversion between ground state, highly populated conformers and less populated higher energy structures ('excited states') that play critical roles in biochemical processes. Here we describe recent advances in NMR spe...
journal_title:Nature chemical biology
pub_type: 杂志文章,评审
doi:10.1038/nchembio.238
更新日期:2009-11-01 00:00:00
abstract::Genetic screens in cultured human cells represent a powerful unbiased strategy to identify cellular pathways that determine drug efficacy, providing critical information for clinical development. We used insertional mutagenesis-based screens in haploid cells to identify genes required for the sensitivity to lasonolide...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-019-0447-7
更新日期:2020-02-01 00:00:00
abstract::Cholinesterase inhibitors, the current frontline symptomatic treatment for Alzheimer's disease (AD), are associated with low efficacy and adverse effects. M1 muscarinic acetylcholine receptors (M1 mAChRs) represent a potential alternate therapeutic target; however, drug discovery programs focused on this G protein-cou...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-019-0453-9
更新日期:2020-03-01 00:00:00
abstract::Only 25% of bacterial membrane transporters have functional annotation owing to the difficulty of experimental study and of accurate prediction of their function. Here we report a sequence-independent method for high-throughput mining of novel transporters. The method is based on ligand-responsive biosensor systems th...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2189
更新日期:2016-12-01 00:00:00
abstract::Cell-permeable small molecules that inhibit their targets on fast timescales are powerful probes of cell-division mechanisms. Such inhibitors have been identified using phenotype-based screens with chemical libraries. However, the characteristics of compound libraries needed to effectively span cell-division phenotype...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio826
更新日期:2006-11-01 00:00:00
abstract::Ferroptosis is a form of regulated necrotic cell death controlled by glutathione peroxidase 4 (GPX4). At present, mechanisms that could predict sensitivity and/or resistance and that may be exploited to modulate ferroptosis are needed. We applied two independent approaches-a genome-wide CRISPR-based genetic screen and...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2239
更新日期:2017-01-01 00:00:00
abstract::We report that simple glycoside donors can drastically shift the equilibria of glycosyltransferase-catalyzed reactions, transforming NDP-sugar formation from an endothermic to an exothermic process. To demonstrate the utility of this thermodynamic adaptability, we highlight the glycosyltransferase-catalyzed synthesis ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.638
更新日期:2011-08-21 00:00:00
abstract::Post-transcriptional RNA modifications can be dynamic and might have functions beyond fine-tuning the structure and function of RNA. Understanding these RNA modification pathways and their functions may allow researchers to identify new layers of gene regulation at the RNA level. ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.482
更新日期:2010-12-01 00:00:00
abstract::Propofol is the most important intravenous general anesthetic in current clinical use. It acts by potentiating GABAA (γ-aminobutyric acid type A) receptors, but where it binds to this receptor is not known and has been a matter of some debate. We synthesized a new propofol analog photolabeling reagent whose biological...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1340
更新日期:2013-11-01 00:00:00
abstract::Small-molecule inhibitors of kinesin-5 (refs. 1-3), a protein essential for eukaryotic cell division, represent alternatives to antimitotic agents that target tubulin. While tubulin is needed for multiple intracellular processes, the known functions of kinesin-5 are limited to dividing cells, making it likely that kin...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio812
更新日期:2006-09-01 00:00:00
abstract::The potential utility of synthetic macrocycles (MCs) as drugs, particularly against low-druggability targets such as protein-protein interactions, has been widely discussed. There is little information, however, to guide the design of MCs for good target protein-binding activity or bioavailability. To address this kno...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1584
更新日期:2014-09-01 00:00:00
abstract::Using linewidth and spinning sideband intensities of lipid hydrocarbon chain resonances in proton magic angle spinning NMR spectra, we detected the temperature-dependent phase state of naturally occurring lipids of intact influenza virus without exogenous probes. Increasingly, below 41 degrees C ordered and disordered...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.77
更新日期:2008-04-01 00:00:00
abstract::Excessive aggregation of proteins has a major impact on cell fate and is a hallmark of amyloid diseases in humans. To resolve insoluble deposits and to maintain protein homeostasis, all cells use dedicated protein disaggregation, protein folding and protein degradation factors. Despite intense recent research, the und...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1931
更新日期:2015-11-01 00:00:00
abstract::The serotonin6 receptor (5-HT6R) is a promising target for treating cognitive deficits of schizophrenia often linked to alterations of neuronal development. This receptor controls neurodevelopmental processes, but the signaling mechanisms involved remain poorly understood. Using a proteomic strategy, we show that 5-HT...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1547
更新日期:2014-07-01 00:00:00
abstract::Binding to the primary receptor, CD4, triggers conformational changes in the metastable HIV-1 envelope glycoprotein (Env) trimer ((gp120-gp41)3) that are important for virus entry into host cells. These changes include an 'opening' of the trimer, creation of a binding site for the CCR5 co-receptor and formation and/or...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1623
更新日期:2014-10-01 00:00:00
abstract::Identification of endogenous glycan-binding ligands for cell-surface receptors has been difficult. Incorporation of a photoactive sialic acid analog into B-cell surface glycoproteins suggests that CD22 molecules may cluster by binding carbohydrate antigens on neighboring CD22 molecules. ...
journal_title:Nature chemical biology
pub_type: 评论,新闻
doi:10.1038/nchembio0705-69
更新日期:2005-07-01 00:00:00
abstract::As a field, lipidomics is in its infancy, yet it has already begun to influence lipid biochemistry in myriad ways. As with other omic technologies, the field is driven by advances in analytical chemistry, particularly by mass spectrometry. At the heart of a renaissance in lipid biochemistry, systems biology is being u...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio0909-602
更新日期:2009-09-01 00:00:00
abstract::Proteoglycans are complex glycoconjugates that regulate critical biological pathways in all higher organisms. Bikunin, the simplest proteoglycan, with a single glycosaminoglycan chain, is a serine protease inhibitor used to treat acute pancreatitis. Unlike nucleic acids and proteins, whose synthesis is template driven...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.673
更新日期:2011-10-09 00:00:00
abstract::Hundreds of human proteins are modified by reversible palmitoylation of cysteine residues (S-palmitoylation), but the regulation of depalmitoylation is poorly understood. Here, we develop 'depalmitoylation probes' (DPPs), small-molecule fluorophores, to monitor the endogenous activity levels of 'erasers' of S-palmitoy...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2262
更新日期:2017-02-01 00:00:00
abstract::The broad study of histone deacetylases in chemistry, biology and medicine relies on tool compounds to derive mechanistic insights. A phylogenetic analysis of class I and II histone deacetylases (HDACs) as targets of a comprehensive, structurally diverse panel of inhibitors revealed unexpected isoform selectivity even...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.313
更新日期:2010-03-01 00:00:00
abstract::Lipid rafts in plasma membranes have emerged as possible platforms for the entry of HIV and other viruses into cells. However, little is known about how lipid phase heterogeneity contributes to viral entry because of the fine-grained and still poorly understood complexity of biological membranes. We used model systems...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1800
更新日期:2015-06-01 00:00:00
abstract::Changes in the cellular environment modulate protein energy landscapes to drive important biology, with consequences for signaling, allostery and other vital processes. The effects of ubiquitination are particularly important because of their potential influence on degradation by the 26S proteasome. Moreover, proteaso...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/s41589-020-0556-3
更新日期:2020-08-01 00:00:00
abstract::Protein kinase signaling along the kinetochore-centromere axis is crucial to assure mitotic fidelity, yet the details of its spatial coordination are obscure. Here, we examined how pools of human Polo-like kinase 1 (Plk1) within this axis control signaling events to elicit mitotic functions. To do this, we restricted ...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2060
更新日期:2016-06-01 00:00:00
abstract::The eukaryotic replicative DNA polymerases (Pol α, δ and ɛ) and the major DNA mutagenesis enzyme Pol ζ contain two conserved cysteine-rich metal-binding motifs (CysA and CysB) in the C-terminal domain (CTD) of their catalytic subunits. Here we demonstrate by in vivo and in vitro approaches the presence of an essential...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.721
更新日期:2011-11-27 00:00:00
abstract::The catalytic activity of many protein kinases is controlled by conformational changes of a conserved Asp-Phe-Gly (DFG) motif. We used an infrared probe to track the DFG motif of the mitotic kinase Aurora A (AurA) and found that allosteric activation by the spindle-associated protein Tpx2 involves an equilibrium shift...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.2296
更新日期:2017-04-01 00:00:00
abstract::Concatenation of engineered biocatalysts into multistep pathways markedly increases their utility, but the development of generalizable assembly methods remains a major challenge. Herein we evaluate 'bioretrosynthesis', which is an application of the retrograde evolution hypothesis, for biosynthetic pathway constructi...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio.1494
更新日期:2014-05-01 00:00:00
abstract::Nitrite represents a bioactive reservoir of nitric oxide (NO) that may modulate vasodilation, respiration and cytoprotection after ischemia-reperfusion injury. Although nitrite formation is thought to occur via reaction of NO with oxygen, this third-order reaction cannot compete kinetically with the reaction of NO wit...
journal_title:Nature chemical biology
pub_type: 杂志文章
doi:10.1038/nchembio813
更新日期:2006-09-01 00:00:00