Abstract：
:Hundreds of human proteins are modified by reversible palmitoylation of cysteine residues (S-palmitoylation), but the regulation of depalmitoylation is poorly understood. Here, we develop 'depalmitoylation probes' (DPPs), small-molecule fluorophores, to monitor the endogenous activity levels of 'erasers' of S-palmitoylation, acylprotein thioesterases (APTs). Live-cell analysis with DPPs reveals rapid growth-factor-mediated inhibition of the depalmitoylation activity of APTs, exposing a novel regulatory mechanism of dynamic lipid signaling.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Kathayat RS,Elvira PD,Dickinson BCdoi
10.1038/nchembio.2262subject
Has Abstractpub_date
2017-02-01 00:00:00pages
150-152issue
2eissn
1552-4450issn
1552-4469pii
nchembio.2262journal_volume
13pub_type
杂志文章abstract：:Only 25% of bacterial membrane transporters have functional annotation owing to the difficulty of experimental study and of accurate prediction of their function. Here we report a sequence-independent method for high-throughput mining of novel transporters. The method is based on ligand-responsive biosensor systems th...
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