Abstract：
:Lipid rafts in plasma membranes have emerged as possible platforms for the entry of HIV and other viruses into cells. However, little is known about how lipid phase heterogeneity contributes to viral entry because of the fine-grained and still poorly understood complexity of biological membranes. We used model systems mimicking HIV envelopes and T cell membranes and found that raft-like liquid-ordered (Lo-phase) lipid domains were necessary and sufficient for efficient membrane targeting and fusion. Interestingly, membrane binding and fusion were low in homogeneous liquid-disordered (Ld-phase) and Lo-phase membranes, indicating that lipid phase heterogeneity is essential. The HIV fusion peptide preferentially targeted to Lo-Ld boundary regions and promoted full fusion at the interface between ordered and disordered lipids. Ld-phase vesicles proceeded only to hemifusion. Thus, we propose that edges but not areas of raft-like ordered lipid domains are vital for HIV entry and membrane fusion.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Yang ST,Kiessling V,Simmons JA,White JM,Tamm LKdoi
10.1038/nchembio.1800subject
Has Abstractpub_date
2015-06-01 00:00:00pages
424-31issue
6eissn
1552-4450issn
1552-4469pii
nchembio.1800journal_volume
11pub_type
杂志文章abstract：:The plant hormone auxin regulates virtually every aspect of plant growth and development. Auxin acts by binding the F-box protein transport inhibitor response 1 (TIR1) and promotes the degradation of the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors. Here we show that efficient auxin binding requires...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.926
更新日期：2012-04-01 00:00:00
abstract：:Tyrian purple, mainly composed of 6,6'-dibromoindigo (6BrIG), is an ancient dye extracted from sea snails and was recently demonstrated as a biocompatible semiconductor material. However, its synthesis remains limited due to uncharacterized biosynthetic pathways and the difficulty of regiospecific bromination. Here, w...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-020-00684-4
更新日期：2021-01-01 00:00:00
abstract：:Targeted drugs are effective when they directly inhibit strong disease drivers, but only a small fraction of diseases feature defined actionable drivers. Alternatively, network-based approaches can uncover new therapeutic opportunities. Applying an integrated phenotypic screening, chemical and phosphoproteomics strate...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2489
更新日期：2017-12-01 00:00:00
abstract：: ...
journal_title：Nature chemical biology
pub_type： 评论,杂志文章
doi：10.1038/s41589-018-0073-9
更新日期：2018-06-01 00:00:00
abstract：:Receptor-mediated endocytosis is an integral part of signal transduction as it mediates signal attenuation and provides spatial and temporal dimensions to signaling events. One of the best-studied leucine-rich repeat receptor-like kinases in plants, BRASSINOSTEROID INSENSITIVE 1 (BRI1), perceives its ligand, the brass...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.958
更新日期：2012-05-06 00:00:00
abstract：:Enzyme-catalyzed peptide ligation is a powerful tool for site-specific protein bioconjugation, but stringent enzyme-substrate specificity limits its utility. We developed an approach for comprehensively characterizing peptide ligase specificity for N termini using proteome-derived peptide libraries. We used this strat...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2521
更新日期：2018-01-01 00:00:00
abstract：:Gentamicin B (GB), a valuable starting material for the preparation of the semisynthetic aminoglycoside antibiotic isepamicin, is produced in trace amounts by the wild-type Micromonospora echinospora. Though the biosynthetic pathway to GB has remained obscure for decades, we have now identified three hidden pathways t...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-018-0203-4
更新日期：2019-03-01 00:00:00
abstract：:Biodiversity, or the relative abundance of species, measures the persistence of an ecosystem. To better understand its modulation, we analyzed the spatial and temporal dynamics of a synthetic, chemical-mediated ecosystem that consisted of two engineered Escherichia coli populations. Depending on the specific experimen...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.244
更新日期：2009-12-01 00:00:00
abstract：:The Watson-Crick base pairs between the 3'-terminal end of tRNAs and ribosomal RNA in the peptidyl transferase center are universally conserved. Here, we report that the introduction of compensatory mutations to Escherichia coli RNAs in this site leads to an orthogonal system independent of the wild-type counterpart, ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1549
更新日期：2014-07-01 00:00:00
abstract：:To maximize a desired product, metabolic engineers typically express enzymes to high, constant levels. Yet, permanent pathway activation can have undesirable consequences including competition with essential pathways and accumulation of toxic intermediates. Faced with similar challenges, natural metabolic systems comp...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-019-0284-8
更新日期：2019-06-01 00:00:00
abstract：:The potential utility of synthetic macrocycles (MCs) as drugs, particularly against low-druggability targets such as protein-protein interactions, has been widely discussed. There is little information, however, to guide the design of MCs for good target protein-binding activity or bioavailability. To address this kno...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1584
更新日期：2014-09-01 00:00:00
abstract：:N(1)-Methyladenosine (m(1)A) is a prevalent post-transcriptional RNA modification, yet little is known about its abundance, topology and dynamics in mRNA. Here, we show that m(1)A is prevalent in Homo sapiens mRNA, which shows an m(1)A/A ratio of ∼0.02%. We develop the m(1)A-ID-seq technique, based on m(1)A immunoprec...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2040
更新日期：2016-05-01 00:00:00
abstract：:N-Acetylglucosamine β-O-linked to nucleocytoplasmic proteins (O-GlcNAc) is implicated in the regulation of gene expression in organisms, from humans to Drosophila melanogaster. Within Drosophila, O-GlcNAc transferase (OGT) is one of the Polycomb group proteins (PcGs) that act through Polycomb group response elements (...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2247
更新日期：2017-02-01 00:00:00
abstract：:Naturally produced polybrominated diphenyl ethers (PBDEs) pervade the marine environment and structurally resemble toxic man-made brominated flame retardants. PBDEs bioaccumulate in marine animals and are likely transferred to the human food chain. However, the biogenic basis for PBDE production in one of their most p...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2330
更新日期：2017-05-01 00:00:00
abstract：:SB269652 is to our knowledge the first drug-like allosteric modulator of the dopamine D2 receptor (D2R), but it contains structural features associated with orthosteric D2R antagonists. Using a functional complementation system to control the identity of individual protomers within a dimeric D2R complex, we converted ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1593
更新日期：2014-09-01 00:00:00
abstract：:N6-Methyladenosine (m6A) RNA modification is present in messenger RNAs (mRNA), ribosomal RNAs (rRNA), and spliceosomal RNAs (snRNA) in humans. Although mRNA m6A modifications have been extensively studied and shown to play critical roles in many cellular processes, the identity of m6A methyltransferases for rRNAs and ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-018-0184-3
更新日期：2019-01-01 00:00:00
abstract：:This Commentary clarifies the meaning of the funnel diagram, which has been widely cited in papers on protein folding. To aid in the analysis of the funnel diagram, this Commentary reviews historical approaches to understanding the mechanism of protein folding. The primary role of free energy in protein folding is dis...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.565
更新日期：2011-06-17 00:00:00
abstract：:Regulation of gene expression in response to the changing environment is critical for cell survival. For instance, binding of macrolide antibiotics to the ribosome promotes translation arrest at the leader open reading frames ermCL and ermBL, which is necessary for inducing the antibiotic resistance genes ermC and erm...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1998
更新日期：2016-03-01 00:00:00
abstract：:Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the presence of a cell-permeable small molecule by inserting an evolved 4-hydroxytamoxifen-responsive i...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1793
更新日期：2015-05-01 00:00:00
abstract：:Nontranslational functions of vertebrate aminoacyl tRNA synthetases (aaRSs), which catalyze the production of aminoacyl-tRNAs for protein synthesis, have recently been discovered. Although these new functions were thought to be 'moonlighting activities', many are as critical for cellular homeostasis as their activity ...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio.1158
更新日期：2013-03-01 00:00:00
abstract：:In this study, we demonstrate the feasibility of expanding the genetic code of Escherichia coli using its own tryptophanyl-tRNA synthetase and tRNA (TrpRS-tRNATrp) pair. This was made possible by first functionally replacing this endogenous pair with an E. coli-optimized counterpart from Saccharomyces cerevisiae, and ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2312
更新日期：2017-04-01 00:00:00
abstract：:Although the protein synthesis inhibitor cycloheximide (CHX) has been known for decades, its precise mechanism of action remains incompletely understood. The glutarimide portion of CHX is seen in a family of structurally related natural products including migrastatin, isomigrastatin and lactimidomycin (LTM). We found ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.304
更新日期：2010-03-01 00:00:00
abstract：:Cyclin-dependent kinase 9 (CDK9), an important regulator of transcriptional elongation, is a promising target for cancer therapy, particularly for cancers driven by transcriptional dysregulation. We characterized NVP-2, a selective ATP-competitive CDK9 inhibitor, and THAL-SNS-032, a selective CDK9 degrader consisting ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2538
更新日期：2018-02-01 00:00:00
abstract：:The actin cytoskeleton supports diverse cellular processes such as endocytosis, oriented growth, adhesion and migration. The dynamic nature of the cytoskeleton, however, has made it difficult to define the roles of the many accessory molecules that modulate actin organization, especially the multifunctional adapter pr...
journal_title：Nature chemical biology
pub_type： 信件
doi：10.1038/nchembio755
更新日期：2006-01-01 00:00:00
abstract：: ...
journal_title：Nature chemical biology
pub_type： 评论,杂志文章
doi：10.1038/s41589-018-0199-9
更新日期：2019-01-01 00:00:00
abstract：:Mycobacterium tuberculosis and Yersinia pestis, the causative agents of tuberculosis and plague, respectively, are pathogens with serious ongoing impact on global public health and potential use as agents of bioterrorism. Both pathogens have iron acquisition systems based on siderophores, secreted iron-chelating compo...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio706
更新日期：2005-06-01 00:00:00
abstract：:Ferroptosis is a form of regulated necrotic cell death controlled by glutathione peroxidase 4 (GPX4). At present, mechanisms that could predict sensitivity and/or resistance and that may be exploited to modulate ferroptosis are needed. We applied two independent approaches-a genome-wide CRISPR-based genetic screen and...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2239
更新日期：2017-01-01 00:00:00
abstract：:Foldamers are sequence-specific oligomers akin to peptides, proteins and oligonucleotides that fold into well-defined three-dimensional structures. They offer the chemical biologist a broad pallet of building blocks for the construction of molecules that test and extend our understanding of protein folding and functio...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio876
更新日期：2007-05-01 00:00:00
abstract：:The endocannabinoid 2-arachidonoylglycerol (2-AG) is biosynthesized by diacylglycerol lipases DAGLα and DAGLβ. Chemical probes to perturb DAGLs are needed to characterize endocannabinoid function in biological processes. Here we report a series of 1,2,3-triazole urea inhibitors, along with paired negative-control and ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1105
更新日期：2012-12-01 00:00:00
abstract：:Accumulation of unfolded proteins within the endoplasmic reticulum (ER) of eukaryotic cells leads to an unfolded protein response (UPR) that either restores homeostasis or commits the cells to apoptosis. Tools traditionally used to study the UPR are proapoptotic and thus confound analysis of long-term cellular respons...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1638
更新日期：2014-11-01 00:00:00
