Abstract：
:The Watson-Crick base pairs between the 3'-terminal end of tRNAs and ribosomal RNA in the peptidyl transferase center are universally conserved. Here, we report that the introduction of compensatory mutations to Escherichia coli RNAs in this site leads to an orthogonal system independent of the wild-type counterpart, as demonstrated via the production of two peptide sequences from a single mRNA. This work thus identifies a new way to reprogram the genetic code.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Terasaka N,Hayashi G,Katoh T,Suga Hdoi
10.1038/nchembio.1549subject
Has Abstractpub_date
2014-07-01 00:00:00pages
555-7issue
7eissn
1552-4450issn
1552-4469pii
nchembio.1549journal_volume
10pub_type
杂志文章abstract：:Transcription factors have diverse roles during embryonic development, combinatorially controlling cellular states in a spatially and temporally defined manner. Resolving the dynamic transcriptional profiles that underlie these patterning processes is essential for understanding embryogenesis at the molecular level. H...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.772
更新日期：2012-01-29 00:00:00
abstract：:The chemical species involved in H2S signaling remain elusive despite the profound and pleiotropic physiological effects elicited by this molecule. The dominant candidate mechanism for sulfide signaling is persulfidation of target proteins. However, the relatively poor reactivity of H2S toward oxidized thiols, such as...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1834
更新日期：2015-07-01 00:00:00
abstract：:MicroRNAs are evolutionarily conserved small, noncoding RNAs that regulate diverse biological processes. Due to their essential regulatory roles, microRNA biogenesis is tightly regulated, where protein factors are often found to interact with specific primary and precursor microRNAs for regulation. Here, using NMR rel...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-020-00667-5
更新日期：2021-01-01 00:00:00
abstract：:Thiazole/oxazole-modified microcins (TOMMs) encompass a recently defined class of ribosomally synthesized natural products with a diverse set of biological activities. Although TOMM biosynthesis has been investigated for over a decade, the mechanism of heterocycle formation by the synthetase enzymes remains poorly und...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.944
更新日期：2012-04-22 00:00:00
abstract：:The molecular forces that drive structural transitions between the open and closed states of channels and transporters are not well understood. The gate of the OmpA channel is formed by the central Glu52-Arg138 salt bridge, which can open to form alternate ion pairs with Lys82 and Glu128. To gain deeper insight into t...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio827
更新日期：2006-11-01 00:00:00
abstract：:Natural enzymes have evolved to perform their cellular functions under complex selective pressures, which often require their catalytic activities to be regulated by other proteins. We contrasted a natural enzyme, LovD, which acts on a protein-bound (LovF) acyl substrate, with a laboratory-generated variant that was t...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1503
更新日期：2014-06-01 00:00:00
abstract：:Cysteine dioxygenase (CDO) plays an essential role in sulfur metabolism by regulating homeostatic levels of cysteine. Human CDO contains a post-translationally generated Cys93-Tyr157 cross-linked cofactor. Here, we investigated this Cys-Tyr cross-linking by incorporating unnatural tyrosines in place of Tyr157 via a ge...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-018-0085-5
更新日期：2018-09-01 00:00:00
abstract：:Virtually all cells in the body have an intracellular clockwork based on a negative feedback mechanism. The circadian timekeeping system in mammals is a hierarchical multi-oscillator network, with the suprachiasmatic nuclei (SCN) acting as the central pacemaker. The SCN synchronizes to daily light-dark cycles and coor...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio.2007.37
更新日期：2007-10-01 00:00:00
abstract：:Liver X receptors (Lxrα and Lxrβ) are ligand-dependent nuclear receptors critical for ventral midbrain neurogenesis in vivo. However, no endogenous midbrain Lxr ligand has so far been identified. Here we used LC/MS and functional assays to identify cholic acid as a new Lxr ligand. Moreover, 24(S),25-epoxycholesterol (...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1156
更新日期：2013-02-01 00:00:00
abstract：:Bacteria are thought to cope with fluctuating environmental solute concentrations primarily by adjusting the osmolality of their cytoplasm. To obtain insights into the underlying metabolic adaptations, we analyzed the global metabolic response of Escherichia coli to sustained hyperosmotic stress using nontargeted mass...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1437
更新日期：2014-04-01 00:00:00
abstract：:Proper gene expression is essential for the survival of every cell. Once thought to be a passive transporter of genetic information, RNA has recently emerged as a key player in nearly every pathway in the cell. A full description of its structure is critical to understanding RNA function. Decades of research have focu...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio.1958
更新日期：2015-12-01 00:00:00
abstract：:Hundreds of cellular proteins require iron cofactors for activity, and cells express systems for their assembly and distribution. Molecular details of the cytosolic iron pool used for iron cofactors are lacking, but iron chaperones of the poly(rC)-binding protein (PCBP) family play a key role in ferrous ion distributi...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-019-0330-6
更新日期：2019-09-01 00:00:00
abstract：:The MerR-family transcription factors (TFs) are a large group of bacterial proteins responding to cellular metal ions and multiple antibiotics by binding within central RNA polymerase-binding regions of a promoter. While most TFs alter transcription through protein-protein interactions, MerR TFs are capable of reshapi...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-020-00653-x
更新日期：2021-01-01 00:00:00
abstract：:The potential utility of synthetic macrocycles (MCs) as drugs, particularly against low-druggability targets such as protein-protein interactions, has been widely discussed. There is little information, however, to guide the design of MCs for good target protein-binding activity or bioavailability. To address this kno...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1584
更新日期：2014-09-01 00:00:00
abstract：:Mitochondrial electron transport drives ATP synthesis but also generates reactive oxygen species, which are both cellular signals and damaging oxidants. Superoxide production by respiratory complex III is implicated in diverse signaling events and pathologies, but its role remains controversial. Using high-throughput ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1910
更新日期：2015-11-01 00:00:00
abstract：:Sulfoquinovose is produced by photosynthetic organisms at a rate of 10(10) tons per annum and is degraded by bacteria as a source of carbon and sulfur. We have identified Escherichia coli YihQ as the first dedicated sulfoquinovosidase and the gateway enzyme to sulfoglycolytic pathways. Structural and mutagenesis studi...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2023
更新日期：2016-04-01 00:00:00
abstract：:This Commentary clarifies the meaning of the funnel diagram, which has been widely cited in papers on protein folding. To aid in the analysis of the funnel diagram, this Commentary reviews historical approaches to understanding the mechanism of protein folding. The primary role of free energy in protein folding is dis...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.565
更新日期：2011-06-17 00:00:00
abstract：:Learning regulatory networks from genomics data is an important problem with applications spanning all of biology and biomedicine. Functional genomics projects offer a cost-effective means of greatly expanding the completeness of our regulatory models, and for some prokaryotic organisms they offer a means of learning ...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio.122
更新日期：2008-11-01 00:00:00
abstract：:The resistance of Caenorhabditis elegans to pharmacological perturbation limits its use as a screening tool for novel small bioactive molecules. One strategy to improve the hit rate of small-molecule screens is to preselect molecules that have an increased likelihood of reaching their target in the worm. To learn whic...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.380
更新日期：2010-07-01 00:00:00
abstract：:The catalytic activity of many protein kinases is controlled by conformational changes of a conserved Asp-Phe-Gly (DFG) motif. We used an infrared probe to track the DFG motif of the mitotic kinase Aurora A (AurA) and found that allosteric activation by the spindle-associated protein Tpx2 involves an equilibrium shift...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2296
更新日期：2017-04-01 00:00:00
abstract：:Using linewidth and spinning sideband intensities of lipid hydrocarbon chain resonances in proton magic angle spinning NMR spectra, we detected the temperature-dependent phase state of naturally occurring lipids of intact influenza virus without exogenous probes. Increasingly, below 41 degrees C ordered and disordered...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.77
更新日期：2008-04-01 00:00:00
abstract：:Methylation of nucleotides in ribosomal RNAs (rRNAs) is a ubiquitous feature that occurs in all living organisms. Identification of all enzymes responsible for rRNA methylation, as well as mapping of all modified rRNA residues, is now complete for a number of model species, such as Escherichia coli and Saccharomyces c...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio.2569
更新日期：2018-02-14 00:00:00
abstract：:The rise of antibiotic resistance demands the acceleration of molecular diversification strategies to inspire new chemical entities for antibiotic medicines. We report here on the large-scale engineering of ribosomally synthesized and post-translationally modified antimicrobial peptides carrying the ring-forming amino...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-019-0250-5
更新日期：2019-05-01 00:00:00
abstract：:Small molecules stabilize specific protein conformations from a larger ensemble, enabling molecular switches that control diverse cellular functions. We show here that the converse also holds true: the conformational state of the estrogen receptor can direct distinct orientations of the bound ligand. 'Gain-of-alloster...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.451
更新日期：2010-11-01 00:00:00
abstract：:Higher plants resist the forces of gravity and powerful lateral forces through the cumulative strength of the walls that surround individual cells. These walls consist mainly of cellulose, noncellulosic polysaccharides and lignin, in proportions that depend upon the specific functions of the cell and its stage of deve...
journal_title：Nature chemical biology
pub_type： 杂志文章,评审
doi：10.1038/nchembio.439
更新日期：2010-10-01 00:00:00
abstract：:To maximize a desired product, metabolic engineers typically express enzymes to high, constant levels. Yet, permanent pathway activation can have undesirable consequences including competition with essential pathways and accumulation of toxic intermediates. Faced with similar challenges, natural metabolic systems comp...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/s41589-019-0284-8
更新日期：2019-06-01 00:00:00
abstract：:The P1B-ATPases, which couple cation transport across membranes to ATP hydrolysis, are central to metal homeostasis in all organisms. An important feature of P1B-ATPases is the presence of soluble metal binding domains (MBDs) that regulate transport activity. Only one type of MBD has been characterized extensively, bu...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.1863
更新日期：2015-09-01 00:00:00
abstract：: ...
journal_title：Nature chemical biology
pub_type： 评论,杂志文章
doi：10.1038/s41589-019-0230-9
更新日期：2019-03-01 00:00:00
abstract：:RNA capping and decapping are thought to be distinctive features of eukaryotes. The redox cofactor NAD was recently discovered to be attached to small regulatory RNAs in bacteria in a cap-like manner, and Nudix hydrolase NudC was found to act as a NAD-decapping enzyme in vitro and in vivo. Here, crystal structures of ...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio.2132
更新日期：2016-09-01 00:00:00
abstract：:Enzymes that use the cofactor thiamin diphosphate (ThDP, 1), the biologically active form of vitamin B(1), are involved in numerous metabolic pathways in all organisms. Although a theory of the cofactor's underlying reaction mechanism has been established over the last five decades, the three-dimensional structures of...
journal_title：Nature chemical biology
pub_type： 杂志文章
doi：10.1038/nchembio788
更新日期：2006-06-01 00:00:00
