The science of sharing and the sharing of science.

abstract：:All but two genes involved in the ergosterol biosynthetic pathway in Saccharomyces cerevisiae have been cloned, and their corresponding mutants have been described. The remaining genes encode the C-3 sterol dehydrogenase (C-4 decarboxylase) and the 3-keto sterol reductase and in concert with the C-4 sterol methyloxida...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gachotte D,Barbuch R,Gaylor J,Nickel E,Bard M

更新日期：1998-11-10 00:00:00

abstract：:The Hyal-1 locus, which we have previously described and mapped to mouse chromosome 9, influences the serum levels and molecular weight forms of hyaluronidase. We have also shown that the growth of two transplantable tumors, the 3LL carcinoma and the B16F10 melanoma, is influenced by the alleles at Hyal-1, in that the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Maeyer-Guignard J,Lauret E,Eusèbe L,De Maeyer E

更新日期：1993-06-15 00:00:00

abstract：:The understanding of mechanisms controlling zinc absorption and metabolism at the molecular level has advanced recently. Kinetics of zinc transport have been investigated for many years, but only recently have genes coding for proteins thought to be involved in the transport process been cloned. Four putative zinc tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMahon RJ,Cousins RJ

更新日期：1998-04-28 00:00:00

abstract：:The thymidine analog fialuridine deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil (FIAU) was toxic in trials for chronic hepatitis B infection. One mechanism postulated that defective mtDNA replication was mediated through inhibition of DNA polymerase-gamma (DNA pol-gamma), by FIAU triphosphate (FIALTP) or by trip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis W,Levine ES,Griniuviene B,Tankersley KO,Colacino JM,Sommadossi JP,Watanabe KA,Perrino FW

更新日期：1996-04-16 00:00:00

abstract：:We sublocalized the yeast nucleoporin Nup82 to the cytoplasmic side of the nuclear pore complex (NPC) by immunoelectron microscopy. Moreover, by in vitro binding assays we showed that Nup82 interacts with the C-terminal region of Nup159, a yeast nucleoporin that previously was also localized to the cytoplasmic side of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hurwitz ME,Strambio-de-Castillia C,Blobel G

更新日期：1998-09-15 00:00:00

abstract：:The central role of cyclin-dependent kinases (CDKs) in cell cycle regulation makes them a promising target for studying inhibitory molecules that can modify the degree of cell proliferation. The discovery of specific inhibitors of CDKs such as polyhydroxylated flavones has opened the way to investigation and design of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Azevedo WF Jr,Mueller-Dieckmann HJ,Schulze-Gahmen U,Worland PJ,Sausville E,Kim SH

更新日期：1996-04-02 00:00:00

abstract：:Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okerberg ES,Wu J,Zhang B,Samii B,Blackford K,Winn DT,Shreder KR,Burbaum JJ,Patricelli MP

更新日期：2005-04-05 00:00:00

abstract：:We have used germ-line transformation to dissect the cis regulatory elements responsible for the transcriptional control of an internally marked Drosophila chorion gene (s15-P) during development. A 73-base-pair segment of the proximal 5'-flanking DNA contains sequences essential for the tissue-specific expression and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mariani BD,Lingappa JR,Kafatos FC

更新日期：1988-05-01 00:00:00

abstract：:Inhomogeneous ab initio molecular dynamics simulations show that vaporization of MgSiO3 is incongruent and that the vapor phase is dominated by SiO and O2 molecules. The vapor is strongly depleted in Mg at low temperature and approaches the composition of the liquid near the critical point. We find that the liquid-vap...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao B,Stixrude L

更新日期：2018-05-22 00:00:00

abstract：:Fatty acid compositions of phospholipids of heart, liver, kidney, aorta, and serum from rats having streptozotocin-induced diabetes were determined and compared with those of nondiabetic controls. Linoleic and dihomo-gamma-linolenic acids were increased whereas arachidonic acid was decreased in most tissues, suggestin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holman RT,Johnson SB,Gerrard JM,Mauer SM,Kupcho-Sandberg S,Brown DM

更新日期：1983-04-01 00:00:00

abstract：:A discrepancy is explored regarding the spin exchange integral estimated from magnetic field effects on recombination of the bacteriochlorophyll dimer cation (Bchl)(2)+ and the bacteriopheophytin anion Bph(-) and the measured electron transfer rate between Bph and electronically excited (Bchl)(2). In one explanation c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haberkorn R,Michel-Beyerle ME,Marcus RA

更新日期：1979-09-01 00:00:00

abstract：:Understanding how changes in antibiotic consumption affect the prevalence of antibiotic resistance in bacterial pathogens is important for public health. In a number of bacterial species, including Streptococcus pneumoniae, the prevalence of resistance has remained relatively stable despite prolonged selection pressur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehtinen S,Blanquart F,Croucher NJ,Turner P,Lipsitch M,Fraser C

更新日期：2017-01-31 00:00:00

abstract：:The voltage-gated proton channel Hv1 plays a critical role in the fast proton translocation that underlies a wide range of physiological functions, including the phagocytic respiratory burst, sperm motility, apoptosis, and metastatic cancer. Both voltage activation and proton conduction are carried out by a voltage-se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Q,Shen R,Treger JS,Wanderling SS,Milewski W,Siwowska K,Bezanilla F,Perozo E

更新日期：2015-11-03 00:00:00

abstract：:Obese fat pads are frequently undervascularized and hypoxic, leading to increased fibrosis, inflammation, and ultimately insulin resistance. We hypothesized that VEGF-A-induced stimulation of angiogenesis enables sustained and sufficient oxygen and nutrient exchange during fat mass expansion, thereby improving adipose...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun K,Wernstedt Asterholm I,Kusminski CM,Bueno AC,Wang ZV,Pollard JW,Brekken RA,Scherer PE

更新日期：2012-04-10 00:00:00

abstract：:Filtration through the kidney eliminates toxins, manages electrolyte balance, and controls water homeostasis. Reabsorption of water from the luminal fluid of the nephron occurs through aquaporin-2 (AQP2) water pores in principal cells that line the kidney-collecting duct. This vital process is impeded by formation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whiting JL,Ogier L,Forbush KA,Bucko P,Gopalan J,Seternes OM,Langeberg LK,Scott JD

更新日期：2016-07-26 00:00:00

abstract：:We used event-related functional MRI to investigate the neural bases of two categories of mental processes believed to contribute to performance of an alphabetization working memory task: memory storage and memory manipulation. Our delayed-response tasks required memory for the identity and position-in-the-display of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Postle BR,Berger JS,D'Esposito M

更新日期：1999-10-26 00:00:00

abstract：:Stressful events evoke long-term changes in behavioral responses; however, the underlying mechanisms in the brain are not well understood. Previous work has shown that epigenetic changes and immediate-early gene (IEG) induction in stress-activated dentate gyrus (DG) granule neurons play a crucial role in these behavio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saunderson EA,Spiers H,Mifsud KR,Gutierrez-Mecinas M,Trollope AF,Shaikh A,Mill J,Reul JM

更新日期：2016-04-26 00:00:00

abstract：:During the late Pleistocene, early anatomically modern humans coexisted in Europe with the anatomically archaic Neandertals for some thousand years. Under the recent variants of the multiregional model of human evolution, modern and archaic forms were different but related populations within a single evolving species,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caramelli D,Lalueza-Fox C,Vernesi C,Lari M,Casoli A,Mallegni F,Chiarelli B,Dupanloup I,Bertranpetit J,Barbujani G,Bertorelle G

更新日期：2003-05-27 00:00:00

abstract：:An array of photoreceptors including cryptochromes, phototropin, and phytochromes regulates various light responses in plants. Among these photoreceptors, phytochromes perceive red and far-red light by switching between two interconvertible spectral forms (Pr and Pfr). The Pfr form promotes light responses partly by d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paik I,Yang S,Choi G

更新日期：2012-01-24 00:00:00

abstract：:In target tissue extracts, heat shock protein hsp90 has been found associated to all unliganded steroid receptors. Modulation of important functions of these receptors, including prevention of DNA binding and optimization of transcriptional activity, has been attributed to hsp90. However no unequivocal in vivo demonst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang KI,Devin J,Cadepond F,Jibard N,Guiochon-Mantel A,Baulieu EE,Catelli MG

更新日期：1994-01-04 00:00:00

abstract：:Old Italian violins are routinely credited with playing qualities supposedly unobtainable in new instruments. These qualities include the ability to project their sound more effectively in a concert hall-despite seeming relatively quiet under the ear of the player-compared with new violins. Although researchers have l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fritz C,Curtin J,Poitevineau J,Tao FC

更新日期：2017-05-23 00:00:00

abstract：:Gene-environment interactions are important determinants of cancer risk. Traditionally, gene-environment interactions are thought to contribute to tumor-suppressor-gene penetrance by facilitating or inhibiting the acquisition of additional somatic mutations required for tumorigenesis. Here, we demonstrate that a disti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cook JD,Davis BJ,Cai SL,Barrett JC,Conti CJ,Walker CL

更新日期：2005-06-14 00:00:00

abstract：:Disease tolerance, the capacity of tissues to withstand damage caused by a stimulus without a decline in host fitness, varies across tissues, environmental conditions, and physiologic states. While disease tolerance is a known strategy of host defense, its role in noninfectious diseases has been understudied. Here, we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Man K,Bowman C,Braverman KN,Escalante V,Tian Y,Bisanz JE,Ganeshan K,Wang B,Patterson A,Bayrer JR,Turnbaugh PJ,Chawla A

更新日期：2020-12-15 00:00:00

abstract：:Ischemia of the myocardium and lower limbs is a common consequence of arterial disease and a major source of morbidity and mortality in modernized countries. Inducing neovascularization for the treatment of ischemia is an appealing therapeutic strategy for patients for whom traditional treatment modalities cannot be p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jang E,Albadawi H,Watkins MT,Edelman ER,Baker AB

更新日期：2012-01-31 00:00:00

abstract：:Phenylglyoxal is an arginine-specific reagent that inactivates creatine kinase (CK). Previous results suggest that modification of the dimeric enzyme at a single arginine residue per subunit causes complete inactivation accompanied by the loss of nucleotide binding; the actual site of modification was not identified. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood TD,Guan Z,Borders CL Jr,Chen LH,Kenyon GL,McLafferty FW

更新日期：1998-03-31 00:00:00

abstract：:Research on tuberculosis and leprosy was revolutionized by the development of a plasmid transformation system in the fast-growing surrogate, Mycobacterium smegmatis. This transformation system was made possible by the successful isolation of a M. smegmatis mutant strain mc(2)155, whose efficient plasmid transformation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Panas MW,Jain P,Yang H,Mitra S,Biswas D,Wattam AR,Letvin NL,Jacobs WR Jr

更新日期：2014-09-16 00:00:00

abstract：:Heterochromatin protein 1 (HP1), first discovered in Drosophila melanogaster, is a highly conserved chromosomal protein implicated in both heterochromatin formation and gene silencing. We report here characterization of an HP1-interacting protein, heterochromatin protein 2 (HP2), which codistributes with HP1 in the pe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaffer CD,Stephens GE,Thompson BA,Funches L,Bernat JA,Craig CA,Elgin SC

更新日期：2002-10-29 00:00:00

abstract：:The fungus, Phycomyces blakesleeanus, shows many well-defined responses to a number of external stimuli. Genetic analysis shows that at least eight genes are involved in Phycomyces sensory transduction. As a first step toward the molecular analysis of these genes and their products, we have developed a transformation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Revuelta JL,Jayaram M

更新日期：1986-10-01 00:00:00

abstract：:The gene encoding human plakoglobin was mapped to chromosome 17q12-q22. An intragenic restriction fragment length polymorphism was used to localize the plakoglobin gene distal to locus KRT10 and proximal to the marker D17S858. The plakoglobin gene colocalizes with the polymorphic 17q21 marker UM8 on the same cosmid in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aberle H,Bierkamp C,Torchard D,Serova O,Wagner T,Natt E,Wirsching J,Heidkämper C,Montagna M,Lynch HT

更新日期：1995-07-03 00:00:00

abstract：:p53 is a master regulatory, sequence-specific transcription factor that directly controls expression of over 100 genes in response to various stress signals. Transactivation is generally considered to occur through p53 binding to a consensus response element (RE) composed of two 5'-RRRCWWGYYY-3' decamers. Recently, st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Menendez D,Inga A,Resnick MA

更新日期：2010-01-26 00:00:00