Abstract：
AIM:To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD:Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses (UV, CD, IR, MS, 1D, and 2D NMR). The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721. RESULTS:Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J (1), cytochalasin H (2), (22E, 24S)-cerevisterol (3), ergosterol (4), and nicotinic acid (5). Compound 1 had an inhibition rate of 24.4% at 10 μg·mL(-1) and 2 had an IC50 value of 15.0 μg·mL(-1), while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg·mL(-1). CONCLUSION:18-Methoxy cytochalasin J (1), produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.
journal_name
Chin J Nat Medjournal_title
Chinese journal of natural medicinesauthors
Shen L,Luo Q,Shen ZP,Li LY,Zhang XJ,Wei ZQ,Fu Y,Song YC,Tan RXdoi
10.1016/S1875-5364(14)60080-7subject
Has Abstractpub_date
2014-07-01 00:00:00pages
512-6issue
7eissn
2095-6975issn
1875-5364pii
S1875-5364(14)60080-7journal_volume
12pub_type
杂志文章abstract：AIM:To improve the absorption and bioavailability of baicalin using a nanocrystal (or nanosuspension) drug delivery system. METHODS:A tandem, ultrasonic-homogenization-fluid bed drying technology was applied to prepare baicalin-nanocrystal dried powders, and the physicochemical properties of baicalin-nanocrystals were...
journal_title：Chinese journal of natural medicines
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journal_title：Chinese journal of natural medicines
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journal_title：Chinese journal of natural medicines
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journal_title：Chinese journal of natural medicines
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
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更新日期：2020-01-01 00:00:00
abstract：AIM:To investigate the chemical constituents from the leaves of Broussonetia papyrifera. METHODS:The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography and preparative HPLC. Their structures were elucidated on the basis of 1D and 2D NMR anal...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
