Abstract：
:This study engineered β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ to improve biosynthesis of astaxanthin in Escherichia coli. Firstly, crtW was randomly mutated to increase CrtW activities on conversion from β-carotene to astaxanthin. A crtW* mutant with A6T, T105A and L239M mutations has improved 5.35-fold astaxanthin production compared with the wild-type control. Secondly, the expression levels of crtW* and crtZ on chromosomal were balanced by simultaneous modulation RBS regions of their genes using RBS library. The strain RBS54 selected from RBS library, directed the pathway exclusively towards the desired product astaxanthin as predominant carotenoid (99%). Lastly, the number of chromosomal copies of the balanced crtW-crtZ cassette from RBS54 was increased using a Cre-loxP based technique, and a strain with 30 copies of the crtW*-crtZ cassette was selected. This final strain DL-A008 had a 9.8-fold increase of astaxanthin production compared with the wild-type control. Fed-batch fermentation showed that DL-A008 produced astaxanthin as predominant carotenoid (99%) with a specific titer of 0.88 g·L-1 without addition of inducer. In conclusion, through constructing crtW mutation, balancing the expression levels between crtW* and crtZ, and increasing the copy number of the balanced crtW*-crtZ cassette, the activities of β-carotene ketolase and β-carotene hydroxylase were improved for conversion of β-carotene to astaxanthin with higher efficiency. The series of conventional and novel metabolic engineering strategies were designed and applied to construct the astaxanthin hetero-producer strain of E. coli, possibly offering a general approach for the construction of stable hetero-producer strains for other natural products.
journal_name
Chin J Nat Medjournal_title
Chinese journal of natural medicinesauthors
Li D,Li Y,Xu JY,Li QY,Tang JL,Jia SR,Bi CH,Dai ZB,Zhu XN,Zhang XLdoi
10.1016/S1875-5364(20)60005-Xsubject
Has Abstractpub_date
2020-09-01 00:00:00pages
666-676issue
9eissn
2095-6975issn
1875-5364pii
S1875-5364(20)60005-Xjournal_volume
18pub_type
杂志文章abstract：:The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria (MFMC) on PPARγ (Peroxisome Proliferator Activated Receptor gamma) and GLUT-4 (Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL(-1) w...
journal_title：Chinese journal of natural medicines
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
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abstract：:The phytochemical progress on Angelica sinensis (Oliv.) Diels over the past decades is summarized. Since 1970s, 165 chemical constituents, including phthalides, phenylpropanoids, terpenoids and essential oils, aromatic compounds, alkaloids, alkynes, sterols, fatty acids, and polysaccharides have been isolated or detec...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
doi：10.1016/S1875-5364(15)30010-8
更新日期：2015-04-01 00:00:00
abstract：:Anti-influenza Chinese herbal medicines (anti-flu CHMs) have advantages in preventing and treating influenza virus infection. Despite various data on antiviral activities of some anti-flu CHMs have been reported, most of them could not be compared using the standard evaluation methods for antiviral activity. This situ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(16)30095-4
更新日期：2016-10-01 00:00:00
abstract：:Twenty-one lignans including three new ones (1, 2 and 13) were isolated from Justicia procumbens. The chemical structures of the new lignans were determined by spectroscopic means including 1D and 2D NMR analysis. These compounds were evaluated for their cytotoxic and anti-HIV activities. The new secoisolariciresinol ...
journal_title：Chinese journal of natural medicines
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doi：10.1016/S1875-5364(19)30117-7
更新日期：2019-12-01 00:00:00
abstract：:Garcinia, a kind of dry resin secreted by Garcinia hanburyi Hook. F. G., is a traditional Chinese medicine with various biological functions such as detoxification, anti-inflammatory, and anthelmintic activities. Recent studies suggest that garcinia has potential anticancer activity. Increasing evidences indicate that...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
doi：10.1016/S1875-5364(18)30103-1
更新日期：2018-09-01 00:00:00
abstract：:Several chemical compounds can restore pigmentation in vitiligo through mechanisms that vary according to disease etiology. In the present study, we investigated the melanogenic activity of six structurally distinct compounds, namely, scopoletin, kaempferol, chrysin, vitamin D3, piperine, and 6-benzylaminopurine. We d...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(18)30116-X
更新日期：2018-10-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
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更新日期：2017-03-01 00:00:00
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journal_title：Chinese journal of natural medicines
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更新日期：2018-07-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
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更新日期：2014-08-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60080-7
更新日期：2014-07-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60104-7
更新日期：2014-09-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30070-6
更新日期：2019-07-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(20)30075-3
更新日期：2020-08-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60012-1
更新日期：2014-01-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(18)30041-4
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abstract：:Contemporary pharmacological research has demonstrated that puerarin, the most important phytoestrogen extracted from Pueraria lobata(Willd.) Ohwi, has protecting functions on the cardiovascular system, nervous system, osteoporosis, liver injury, and inflammation in vivo and in vitro. Most of these research studies fo...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
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更新日期：2014-06-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(19)30026-3
更新日期：2019-03-01 00:00:00
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journal_title：Chinese journal of natural medicines
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更新日期：2018-04-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
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更新日期：2013-03-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
abstract：:Traditional Chinese medicines (TCMs) are in great demand all over the world, especially in the developing world, for primary health care due to their superior merits such as low cost, minimal side effects, better cultural acceptability, and compatibility with humans. However, Chinese medicines consist of several herbs...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章,评审
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更新日期：2013-11-01 00:00:00
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journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(18)30137-7
更新日期：2018-12-01 00:00:00
abstract：AIM:To investigate the quinoline alkaloids from the roots of Dictamnus angustifolius G.Don ex Sweet (Rutaceae). METHOD:The quinoline alkaloids were isolated by various column chromatographic methods and their structures were elucidated on the basis of spectral analysis. RESULTS:A new quinoline alkaloid, 5-methoxylrob...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60037-6
更新日期：2014-03-01 00:00:00
abstract：:Naodesheng (NDS) formula, which consists of Rhizoma Chuanxiong, Lobed Kudzuvine, Carthamus tinctorius, Radix Notoginseng, and Crataegus pinnatifida, is widely applied for the treatment of cardio/cerebrovascular ischemic diseases, ischemic stroke, and sequelae of cerebral hemorrhage, etc. At present, the studies on NDS...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(18)30029-3
更新日期：2018-01-01 00:00:00
abstract：:In the present study, two new compounds from Ipomoea cairica were identified and demonstrated to have α-glucosidase inhibitory activity. They were isolated by column chromatography on silica gel and sephadex LH-20 and finally purified by prep-HPLC, with their structures being elucidated by spectroscopic methods, such ...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(16)30020-6
更新日期：2016-03-01 00:00:00
abstract：AIM:The compound B19 (C21H22O5) is a newly synthesized, mono-carbonyl analog of curcumin that has exhibited potential antitumor effects. This present study was performed to identify the anti-angiogenic activity of this compound. METHODS AND RESULTS:B19 inhibited migration and tube formation of human umbilical vein end...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60002-9
更新日期：2014-01-01 00:00:00
abstract：:A new γ-alkylated-γ-butyrolactone, named melongenolide A (1), along with nine known compounds were obtained from the roots of Solanum melongena, and their structures were identified as melongenolide A (1), (+)-syringaresinol (2), (+)-lyoniresinol (3), 5,5'-dimethoxy lariciresinol (4), (+)-(7R,8R)-4-hydroxy-3,3',5'-tri...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(15)30068-6
更新日期：2015-09-01 00:00:00
abstract：AIM:JS-38 (mitothiolore), a synthetic version of a metabolite isolated from Xenorhabdus sp., was evaluated for its anti-tumor and white blood cell (WBC) elevating activities. METHOD:These anti-proliferative activities were assessed in vitro using a panel of ten cell lines. The anti-tumor activities were tested in vivo...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(14)60117-5
更新日期：2014-10-01 00:00:00
abstract：:The present study aimed at determining the chemical constituents of Solanum coagulans and their antimicrobial activities. The compounds were isolated by various chromatographic techniques and their structures were elucidated on the basis of extensive spectroscopic analysis, chemical methods, and comparison with report...
journal_title：Chinese journal of natural medicines
pub_type： 杂志文章
doi：10.1016/S1875-5364(16)30033-4
更新日期：2016-04-01 00:00:00
